94 results about "Blood smear" patented technology

A method for counting blood cells in a sample of whole blood. The method comprises the steps of:(a) providing a sample of whole blood;(b) depositing the sample of whole blood onto a slide, e.g., a microscope slide;(c) employing a spreader to create a blood smear;(d) allowing the blood smear to dry on the slide;(e) measuring absorption or reflectance of light attributable to the hemoglobin in the red blood cells in the blood smear on the slide;(f) recording a magnified two-dimensional digital image of the area of analysis identified by the measurement in step (e) as being of suitable thickness for analysis; and(g) collecting, analyzing, and storing data from the magnified two-dimensional digital image.Optionally, steps of fixing and staining of blood cells on the slide can be employed in the method.

A method and apparatus for controlling the processing of a liquid sample, e.g., a blood sample, based on a measurement related to the sample's ability to coat a surface against which the sample is brought into contact while being routinely transported. Preferably, the sample is advanced through a tube, and the amount of sample residue remaining within the tube is measured and correlated to coating process subsequently carried out on the sample, e.g., a process for producing blood-smears on a microscope slide. In a preferred embodiment, the amount of sample residue in a blood-transport tube is used to control the motion profile (i.e., acceleration and velocity) of a drop-spreading member used to spread a blood drop on a microscope slide in an automated slide-making instrument.

A blood cell image display apparatus comprising: a classifier for classifying blood cell images, which are obtained by imaging a blood smear prepared from a blood specimen, in accordance with types of blood cells in the blood cell images; an information receiver for receiving information relating to the blood specimen; a display section; and a display controller for determining a type of blood cell as a display object on the basis of the information relating to the blood specimen, and displaying a blood cell image classified as the determined type on the display section, is disclosed. A specimen analyzing system, a blood cell image display method, and a computer program product are also disclosed.

The invention relates to an automatic peripheral blood pushing device, which can achieve regular and standard blood smears by controlling parameters having directly affection on quality of the blood smears during a pushing process. Particularly, the automatic peripheral blood pushing device can control three degrees of freedom, utilizes a horizontal motor to control inclined included angle between a push slide and a glass slide, and utilizes a left motor to drive a synchronous belt pulley to complete blood pushing action, and adjusts speed and acceleration of the push slide by controlling rotation speed and acceleration of the left motor so as to control lengths of formed blood films. A clearance between the push slide and the horizontal glass slide is adjusted by utilizing an upper motor to drive a lead screw so as to control thicknesses of the formed blood films. Angle, speed and acceleration of the push slide, lengths of blood films and a clearance between the push slide and the glass slide can be adjusted by controlling running of the three motor, thereby manufacturing regular and stand blood smears with different thicknesses.

The invention discloses a device and method for determining parameters of a push piece, a push piece dyeing machine and a method for manufacturing a blood smear. The push piece dyeing machine comprises a blood transmission device, the push piece, a blood characteristic acquisition unit, a push piece parameter determining unit and a motion control unit, wherein the blood characteristic acquisition unit transmits a detection signal to a blood sample transmitted by the blood transmission device and receives a detection signal acting on the blood sample; the push piece parameter determining unit receives the detection signal output by the blood characteristic acquisition unit and determines and outputs the push piece parameters according to the detection signal; the motion control unit receives the push piece parameters output by the push piece parameter determining unit and controls relative motion of the push piece and a blood glass slide according to the push piece parameters. According to the method and the device, the operations of determining the push piece parameters and finishing manufacture of the blood smear can be performed in an independent push piece dyeing system or equipment, the system or the equipment is not required to be in cascade connection with a hematology analyzer to acquire related blood parameters, the process of determining the push piece parameters is simplified, and the complexity of the whole push piece dyeing machine is reduced in the aspects of structure and system operation.

The invention relates to a blood sample detection device, a blood sample detection method and a blood sample detection system. In the invention, a to-be-tested sample and a diluent are added into a counting device. The counting device is controlled to count the diluted to-be-tested sample to obtain a counting result. According to the counting result of the to-be-test sample, sampling parameters and slide pushing parameters are determined to produce a blood smear, thereby improving preparation efficiency and quality of the blood smear. By means of collection of amplified images of the blood smear, morphological feature parameters of the objects in the amplified images are obtained, thereby performing recognition analysis to the object to obtain a morphological feature analytic result of the objects. With the morphological feature analytic result, the morphologies of various cells in the to-be-test sample can be reflected effectively, thereby accurately reflecting true situation of the blood sample.

An apparatus and method of automated blood cell analysis uses technologies from other systems to create a new, robust, improved type of automated microscope, which uses electronic motors and a closed loop control system to minimize ambient factors, such as jarring and temperature changes. Pre-stained blood smear slides are first coated with a thin film of oil and are loaded into a carousel from which they can individually be analyzed. The slides are moved under a low magnification microscope; an optimal area of examination is determined; a focal plane map is calculated for that area, and the positions of white blood cell candidates are computed. The slide is then moved under a high power microscope where a refined focal plane map is computed and the individual white blood cell candidates are imaged. The cells are preclassified and the images are made available for analysis by the technician. Additionally, samples of red blood cells, equivalent to what the technician would do manually, are imaged and presented to the technician for evaluation. The technician may make notes on those cells, both red and white blood cells and archive them.

The invention discloses an automatic medical examination blood smear dyeing machine. According to the invention, a substrate is fixed at the upper part of a frame; a test tube conveyer is arranged in the frame; a test tube suction unit is arranged at the upper part of the substrate; a slide glass conveyer is arranged on the substrate; a sample smearing unit is connected with the slide glass conveyer; a dyeing device is connected with a sample picture device; a controlling unit is arranged on the substrate; a cleaning device is arranged on the test tube suction unit; a waste liquid gathering device is arranged at the lower part of the test tube suction unit; a printing unit is arranged on the substrate; and a network transmission unit is arranged in the controlling unit. The automatic medical examination blood smear dyeing machine can automatically complete operations like uniform mixing and sampling of a blood sample, automatically realizes cleaning via the cleaning device and overcomes the problems of great labor intensity, low detection efficiency, difficulty in guaranteeing smear dyeing quality and the like due to manual or partially machine-aided implementation of the operations in conventional medical examination mechanisms.

The invention relates to an end-to-end blood cell recognition model construction method and application. A data sample set is formed based on a full-view image. An artificial intelligence technology is used for training a blood cell recognition model, and a mature recognition model is finally formed through continuous parameter optimization and error analysis optimization of the model.The model input is a single-view blood smear image, and the output is all cell positions, edges and categories on the image. Full-view blood cell analysis is achieved through a computer, interference of human objective factors is greatly reduced, and objectivity and consistency of inspection results are improved. The blood cell recognition model is intelligent, the software algorithm has a self-learning attribute, the training efficiency of the recognition model is gradually improved along with the increase of high-quality labeled images, and the software recognition and classification accuracy can be continuously optimized.

A blood smear preparation device includes a base; a carrying table for carrying a microscope slide thereon and being supported on the base; a lifting mechanism mounted to the base; a retaining stand suspended from an output end of the lifting mechanism; a spreader holder rotatably suspended from the retaining stand about a second rotating shaft and positioned above the carrying table; a positioning member for positioning the spreader; and a torsion elastic member provided about the second rotating shaft, with a free end of the torsion elastic member abutting against the spreader holder. With the provision of the second rotating shaft and the torsion elastic member, the spreader has a certain degree of flexibility and self-adaptiveness. Even if the spreader or the microscope slide has no good micro-flatness or straightness, the device may automatically adjust the positions of the spreader and the microscope slide to achieve a line contact and a surface contact, and thus ensure the quality of the blood smear.

A blood smear preparation device includes a base; a carrying table for carrying a microscope slide thereon and being supported on the base; a lifting mechanism mounted to the base; a retaining stand suspended from an output end of the lifting mechanism; a spreader holder rotatably suspended from the retaining stand about a second rotating shaft and positioned above the carrying table; a positioning member for positioning the spreader; and a torsion elastic member provided about the second rotating shaft, with a free end of the torsion elastic member abutting against the spreader holder. With the provision of the second rotating shaft and the torsion elastic member, the spreader has a certain degree of flexibility and self-adaptiveness. Even if the spreader or the microscope slide has no good micro-flatness or straightness, the device may automatically adjust the positions of the spreader and the microscope slide to achieve a line contact and a surface contact, and thus ensure the quality of the blood smear.

The invention provides an automatic blood smear preparing device. The automatic blood smear preparing device comprises a rack, the rack includes a support frame and a support platform supported at the support frame. The support platform is provided with the following mechanisms: a smear-entering mechanism, a smearing mechanism arranged at one side of the smear-in mechanism, a dyeing mechanism, a blood sample loading mechanism placed between the dyeing mechanism and the smear-entering mechanism and a glass slide outputting mechanism fixed on the support frame and arranged near the dyeing mechanism. The dyeing mechanism includes a rotatable dyeing plate, a plurality of dyeing boxes used for placing glass slides distributed evenly on the dyeing plate, and a mechanical arm used for putting the glass slides into the dyeing boxes or taking the glass slides from the dyeing boxes. A blood sampling tube platform used for placing blood sampling tubes is fixed at the side lower part of the support frame. The invention is compact in structure, small in size, and achieves automatic smearing and automatic dyeing of blood sample.

A blood cell image display apparatus comprising: a classifier for classifying blood cell images, which are obtained by imaging a blood smear prepared from a blood specimen, in accordance with types of blood cells in the blood cell images; an information receiver for receiving information relating to the blood specimen; a display section; and a display controller for determining a type of blood cell as a display object on the basis of the information relating to the blood specimen, and displaying a blood cell image classified as the determined type on the display section, is disclosed. A specimen analyzing system, a blood cell image display method, and a computer program product are also disclosed.

This invention relates to blood smear leucocyte dye liquid, which is composed of amine salt basic ion surface active agent, polyvinyl pyrrolidon or carbowax and regular leucocyte dye liquid with their constitution proportion as 0.0001%-0.04%,0.0125%-2.5% and 30%-75%. This invention can effectively remove the erythrocyte mixture to properly add the blood thickness to make leucocyte number add within each eye scope.

The invention discloses a subfamily distant hybridization for German mirror carp and megalobrama amblycephala. The method comprises the following steps of artificial dry-process fertilization on the ova of the pubescent German mirror carp and sperms of the megalobrama amblycephala by taking the German mirror carp as a female parent and the megalobrama amblycephala as a male parent, hatching fertilized ova at the water temperature of 19-20 DEG C, and breeding fries in a pond; then, detecting the DNA (deoxyribonucleic acid) content, the chromosome number and the blood cell sizes of the fries by a flow cytometry DNA content determination method, a chromosome ploidy detection method of peripheral blood cell culture and a blood smear method, screening, and then preparing to obtain triploid hybrid fishes and tetraploid hybrid fishes. A tetraploid fish strain which is genetically stable and has four sets of crucian carp chromosomes can be formed by subsequent self-cross selective breeding of the tetraploid hybrid fishes, and the tetraploid fish strain can be used as a resource for breeding the infertile triploid fishes. The method disclosed by the invention has the advantages that not only are the characters of hybrid offspring good but also a good foundation can be laid for variety breeding, and a precious resource is provided for subsequent breeding of new polyploid fishes.

A substrate of blood smear is transparent adhesive tape and device for preparing blood smear is featured as forming a drying-sealed case and a staining sealed case when top and bottom isolation cover are closed in order to carry out drying and staining of blood smear, making transparent adhesive tap move a working position forward when operation is finished.

The invention provides a circulating tumor cell detection recognition method and system. A light source module is used for emitting laser and used for focusing the laser on a sample to be tested; thesample to be tested is a blood smear which is subjected to filtration and non-fluorescent dyeing; an ellipsoid reflector is used for reflecting laser scattered by the sample to be tested to a light receiving surface of a photovoltaic conversion module; the photovoltaic conversion module is used for converting an acquired light signal into an electric signal, and used for transmitting the electricsignal to an image recognition module; the image recognition module is used for generating images of the sample to be tested according to the electric signal, and used for recognizing circulating tumor cells in the images. Since the sample to be tested is only a blood smear which is subjected to filtration and non-fluorescent dyeing without biological specific enrichment, not only is the detectioncost lowered, but also the detection sensitivity, accuracy and detection efficiency can be improved; in addition, as blood is dyed with a non-fluorescent dye when being smeared, the detection sensitivity can be further improved.

The invention relates to a smear device, in particular to an automatic smearing device for blood smear manufacturing. The technical problem is how to design an automatic smearing device for blood smear manufacturing, which can replace manual work to smear, is uniform in blood smearing, can air-dry a blood smear and is high in working efficiency. The automatic smearing device for blood smear manufacturing comprises a supporting frame, a machine box is fixedly connected to the end of the supporting frame, an inclined groove capable of enabling a blood smear to slide down is formed in one side ofthe machine box, and a discharging hopper is installed on the side, close to the inclined groove, outside the machine box. According to the invention, a glass slide is placed on the support plate; blood is dripped on the glass slide, the motor is started to rotate forwards to enable a push piece to move rightwards to make contact with the blood, then the motor is started to rotate backwards, thepush piece moves leftwards to smear the blood on the glass slide, manual smearing is not needed, uneven blood smearing is avoided, air can be blown out through the air blowing opening to air-dry the blood smear, and the working efficiency is high.

The invention relates to a blood smearing machine, a blood smear preparation method, and a device and a method used for determining smearing parameters. The blood smearing machine comprises a sample adding device, a detecting device, a smear, and a controlling device; the controlling device is used for receiving counting results of blood to be tested, determining sample adding parameters based on the counting results, and controlling the sample adding device to add blood to be tested onto a carry sheet glass dropwise based on the sample adding parameters; the detecting device is used for detecting diffusion time of blood to be tested on the carry sheet glass; and the controlling device is also used for determining smearing parameters based on the counting results and diffusion time of blood to be tested, and controlling the smear to move relatively to the carry sheet glass based on the smearing parameters so as to obtain blood smears. The concentration of blood to be tested is reflected by counting results of blood to be tested; the viscosity of blood to be tested is reflected by blood diffusion time; the smearing parameters are determined via combination of the concentration and viscosity of blood to be tested; and blood characteristics of blood to be tested are taken into consideration fully; so that high-quality blood smears can be prepared.

The invention relates to the field of medical instruments, and particularly discloses an automatic manufacturing device of a blood smear. The device comprises a conveying unit, a dropping pipette, a pushing piece, a drying tube and a dyeing tube. The conveying unit comprises a conveying belt and a conveying driving piece, a plurality of placing plates are arranged on the conveying belt at intervals, and annular baffles are arranged at the peripheries of the placing plates and are fixed to the conveying belt. The placing plates are slidably connected with the baffles, and sliding rods are fixedto the bottoms of the placing plates and penetrate through the conveying belt,the dropping pipette and the dyeing tube are both vertically arranged, and the pushing piece, the dropping pipette, the drying tube and the dyeing tube are all located on the placing plates. The device disclosed by the invention can be used for automatically manufacturing the blood smear so as to improve the manufacturing efficiency of the blood smear.

The invention relates to a reticulocyte classification and counting model construction method and application. A reticulocyte classification and counting model is constructed and used for classifyingand respectively counting reticulocytes and other common erythrocytes; the existing mature data set is taken as a first database; a plurality of blood smear micrographs are obtained, and a second database is formed after outline and category labeling of common red blood cells and reticulocytes is conducted; and samples are selected from the first database and the second database to complete two-stage training. Through the reticulocyte classification model, erythrocytes are automatically and efficiently classified and counted, and compared with manual statistics, the efficiency is qualitativelyimproved. The method is fair and objective, eliminates man-made and subjective factors, and has no interference of fatigue and other objective factors. The model has a self-learning attribute, the training efficiency of the recognition model is gradually improved along with the increase of high-quality labeled pictures, and the recognition and classification accuracy of the model can be continuously optimized.

A computer controlled method for detecting and diagnosing a rare cell type in a tissue sample is provided, said method comprising treating the tissue sample such that it generates a first signal indicative of the presence at a location of a rare cell, detecting the first signal, treating the location at which the first signal is detected to generate a second signal indicative of a diagnostically useful cellular characteristic and detecting the second signal. The first signal can be morphological or a color present in a sought cell either before or after staining. The second signal can be generated by in situ PCR or PCR in situ hybridization. In one preferred embodiment, the rare cell type is a fetal cell in a maternal blood tissue sample, said sample consisting of a smear of unenriched maternal blood. In another embodiment, the method is used to diagnose or genotype cancer cells in a blood or tissue biopsy sample.

The invention discloses a blood smear full-view intelligent analysis method which comprises the following steps: acquiring a plurality of original blood smear single-view images, establishing an original blood smear single-view image group, and establishing a blood smear full-view image based on the plurality of original blood smear single-view images; constructing an image restoration model basedon the first training set and the first verification set; constructing an image segmentation model based on the second training set and the second verification set, obtaining a third training set anda third verification set based on the plurality of segmented single blood cell images, and constructing an image recognition model; finally, acquiring a blood cell classification result. Full-view blood cells are analyzed on the basis of an artificial intelligence algorithm, interference of human factors is greatly reduced, objectivity of an inspection result is improved, and blood cell analysisand classification accuracy is high; recognition and analysis can be achieved for picture input meeting the requirements, the algorithm robustness and accuracy are higher than those of a traditional image recognition algorithm, an existing medical examination process is overturned, and the overall time is greatly shortened.

The invention discloses a full-automatic blood smear morphological analysis system based on machine vision and relates to the technical field of medical instruments. The system comprises an optical system unit, an upper computer software processing unit and a lower computer hardware unit; the optical system unit comprises a biological microscopic imaging system and a CCD biological camera; the upper computer software processing unit comprises a user control interface module, a blood smear image processing module and an upper and lower computer communication module; the lower computer hardwareunit comprises a focusing motion module and an image acquisition motion module; automatic focusing is performed through the optical system unit; a blood smear digital image is collected; blood smear image features are recognized and processed through a machine learning algorithm of the upper computer software processing unit; different types of leukocytes are classified and counted; and a processing result is displayed the a graphical user interface; a monocular microscope is matched with a reflective light source, so that an optical structure is greatly simplified, and rapid imaging is realized. The full-automatic blood smear morphological analysis system is high in working efficiency, high in accuracy and high in automation level.

The invention discloses a fully automatic smear pushing dyeing system, and belongs to the field of a medical appliance. The fully automatic smear pushing dyeing system comprises a whole machine base plate, a smear feeding sampling three-axis module, a smear pushing module, a dyeing smear cabin module, a sample feeding smear taking three-axis module, a collection module, a sample position, a glassslide position, a sample feeding needle cleaning groove and a pushing cutter cleaning groove. A set of three-axis mechanical arm is used; the smear feeding sampling action is performed; a smear pushing shaft is used for performing angle regulation; after the smear pushing is completed, the second set of three-axis mechanical arm is used; a blood smear is collected to a dyeing smear with an inclined groove; dyeing is performed; then, cleaning and collection are performed. The fire-new angle regulating structure is used; the dyeing smear cabin structure with an inclined groove is used; the design is reasonable; the repeated performance is good; the reliability is high; a plurality of samples can be treated at the same time; the efficiency is improved; the uncertainty of manual smear pushingis avoided; convenience is provided for the fast diagnosis by medical personnel.

The invention belongs to the technical field of biology, and particularly relates to a compound staining solution of a piroplasma blood smear. The compound staining solution comprises a compound staining solution I and a compound staining solution II, wherein the compound staining solution I comprises 2g Swiss powder, 1.7g giemsa pigment, 50ml glycerol and 1000ml methanol; and the compound staining solution II comprises 2.3g anhydrous sodium dihydrogen phosphate, 0.3g anhydrous monopotassium phosphate and 1000ml distilled water. The staining method comprises the following steps: I, blood smear preparation, II, secondary treatment of the blood smear, and III, staining in the compound staining solution. The compound staining solution and the staining method have a better piroplasma staining effect without the help of high-end instruments and equipment, are economical and practical and have a very excellent effect; the required time is shorter; and no impurity interferes with observation of a piroplasma body.

The invention relates to a disposable doctor blade system. The disposable doctor blade system comprises a disposable doctor blade automatic pushing device, a doctor blade fixing device and a driving mechanism, wherein one side of one end part of the doctor blade fixing device is pivoted to the driving mechanism, and one side of the other end part of the doctor blade fixing device is connected with a driving plate of the driving mechanism, so that the doctor blade fixing device is driven by the driving plate of the driving mechanism to move in a first direction; the doctor blade fixing device comprises a doctor blade fetching position and a doctor blade pushing position; when the doctor blade fixing device is butted with a doctor blade pushing box of the disposable doctor blade automatic pushing device, the doctor blade fixing device is positioned in the doctor blade fetching position, and the disposable doctor blade automatic pushing device can push a disposable doctor blade out of the doctor blade box, and position the disposable doctor blade in the doctor blade fixing device; when the doctor blade fixing device is continuously moved to a preset position in the first direction, the doctor blade fixing device is positioned in the doctor blade pushing position. The disposable doctor blade system has the advantages that by adopting the disposable doctor blade, the repeated cleaning is not needed, the doctor blade can be automatically fetched and pushed, the convenience in use is realized, and the detection efficiency and accuracy are improved. The invention also provides a blood smear automatic manufacturing instrument.

The invention provides a full-automatic high-performance leukocyte segmentation method. In clinical diagnosis and treatment of a patient, classification and counting of white blood cells on a blood smear are very important. However, manual leukocyte classification and counting by hematologists are time-consuming and error-prone. According to the algorithm provided by the invention, a series of processing such as segmentation is carried out on the nuclear region through the zoomed saturation image, and then image processing such as difference is carried out on the green image and the blue imagenormalized by the red image, so that the purpose of automatically and accurately segmenting the cytoplasm region in the sub-image is achieved.

A non-label diagnosis apparatus for a hematologic malignancy may include a 3-D refractive index cell imaging unit configured to generate a 3-D refractive index slide image of a blood smear specimen by capturing a 3-D refractive index image in the form of the blood smear specimen in which blood (including a bone-marrow or other body fluids) of a patent has been smeared on a slide glass, an ROI detection unit configured to sample a suspected cell segment in the blood smear specimen based on the 3-D refractive index slide image and to determine, as ROI patches, cells determined as abnormal cells, and a diagnosis unit configured to determine a sub-classification of a cancer cell corresponding to each of the ROI patches using a cancer cell sub-classification determination model constructed based on a deep learning algorithm and to generate hematologic malignancy diagnosis results by gathering sub-classification results of the ROI patches.