855 results about "Micro imaging" patented technology

Method and implementing apparatus useful in acquiring data on acoustic features within a sample provides a pulsed acoustic probe and a stage system for effecting relative movement between the sample and the probe. The stage system is commanded such that the probe interrogates a non-rectangularly bounded space on the sample surface or within the volume of the sample. Acoustic energy reflected from or transmitted through the sample is sensed. Amplitude signals are developed from the sensed acoustic energy. Data characterizing the developed amplitude signals are stored for subsequent processing.

A miniaturized utility device having integrated optical capabilities for use on a high aspect ratio system, wherein the miniaturized utility device comprises: (a) a micro camera comprising a solid state micro imaging device including, as an integral structure, an imaging array electrically coupled to a conductive pad, wherein the solid state imaging device further includes at least one utility aperture passing therethrough, and a lens optically coupled to the imaging array; and (b) a micro utility instrument configured for coordinated operation with the imaging device at a common local site, wherein the micro utility instrument is configured to perform a designated function viewable, preferably in real-time, via a camera image generated by the micro camera.

The invention provides a laser spectrum analyzer combining a confocal micro-Raman spectrometer with a laser-induced breakdown spectrometer (LIBS). The analyzer comprises a micro-Raman system, a micro LIBS system, a high resolution micro-imaging system, a confocal micro-light path, and a spectrum receiving system with a time resolution function, and automatically switches into a white light micro-imaging observation mode, an automatic focusing mode, a LIBS spectrum working mode, and a Raman spectrum working mode. The significant characteristics of the invention are that compact combination of Raman with LIBS is realized by a micro confocal system; qualitative and quantitative analysis of substance elements at the same minimal position and molecular structure is realized; with the high-resolution imaging function, element spatial discrimination and substance structure chemical analysis can be carried out in micrometers so as to obtain complete information of spatial distribution images of chemical elements, substance structure and physical conditions of a sample.

The invention relates to an in-situ testing machine for the microcosmic performance for a multi-load and multi-physical-field coupling material, belonging to the technical field of material mechanics property testing. The in-situ testing machine comprises a micro/nanometer-precision driving/transmission module, a 'mechanical-electric-thermal-magnetic' loading module and a control module, is integrated with a high-field-depth 3D (three dimensional) micro-imaging lens, a Raman spectrometer and a visual in-situ monitoring module and is capable of dynamically monitoring deformational behaviors, damage mechanisms and performance evolvement rules of the material in a loading process. The in-situ testing machine has the advantages that the whole structure is compact, and the space layout is saved; the loads of four manners including 'stretching/compression-torsion-bending-indentation' can be singly loaded, two or more loads can be combined to be loaded as well, and externally-applied physical fields such as heat, electricity, magnetism and the like can be combined to maximally simulate the real working conditions of components of the material, and effective measures and methods are provided for testing the micromechanical performance of the material under a service-approximating condition.

The invention discloses a multi-mode micro-imaging system and method based on an LED array. The LED array serves as a microscope system light source and generates controllable multi-angle illumination light and a controllable illumination pore diameter, and bright field imaging, dark field imaging and difference phase contrast imaging are achieved. The realizable multi-mode imaging includes the three imaging modes of bright field imaging, dark field imaging and difference phase contrast imaging, and while bright field imaging, dark field imaging and difference phase contrast imaging are achieved, it is unnecessary to add any additional optical elements into the imaging path of a traditional microscope; in this way, an optical system is greatly simplified, and the LED array enables the microscope to have the capacity of flexibly adjusting the illumination pore diameter, the illumination angle and light source coherence.

The invention relates to an automatic micro imaging instrument used for detecting cast-off cells and a detecting method, in particular to an automatic analysis instrument of a uterine neck cell smear and a detecting method. The instrument comprises a microscope, a three dimensional automatic object stage, an automatic film loading device and a control system thereof, a light system, a stable system, a light filter, a computer, a display and a digital camcorder, etc. The detecting method comprises the following steps: the smear is analyzed and colored, then arranged on the automatic object stage which moves up and down to focus automatically, when the automatic object stage moves horizontally, the smear scanning is realized; cell images are amplified by the microscope, picked up and sent to the computer to carry out image processing and analyzing. The detected cells are filtered by two-value filtration to remove the noises, the area and the perimeter are measured, and the impurity is deleted, then the spectrum and other shape parameters of the cells are measured; the measured cells are classified with a pattern recognition algorithm into the cast-off cells, white blood cells, lymphocyte cells and the impurity; after a viewing field is finished, the cells move down automatically, auxiliary diagnostic data is obtained by the computer. The instrument and the detecting method have the advantages of high degree of automation, accurate analysis results and reliability.

The invention discloses a structure illumination super-resolution microscopy imaging system and an imaging method thereof. The imaging system comprises an illumination light source, a rotating structure light generator, a first convergent lens, a light splitter, an objective lens, an object stage, a sample, a second convergent lens, a digital imaging device and a computer. An original image is processed through an imaging image reconstruction algorithm; super-resolution can be achieved only by rotating structured light stripes for four times without phase displacement; the imaging image reconstruction algorithm is based on frequency domain processing instead of non-spatial domain processing; a spectrum process of a super-resolution image of a sample is different from a traditional frequency domain method, structured light directions are not analyzed one by one and all directions are merged for analysis; in order to obtain traditional uniform 2-fold resolution improvement in various directions, the theoretical quantity of original images can be reduced to 4 from 9 of a traditional method; the quantity of the original images is further reduced to 3 and uniform 1.5fold resolution improvement in various direction can be theoretically obtained.

The invention discloses a frequency-shift super-resolution microimaging method based on evanescent field illumination. The method comprises the steps that 1) incident illumination light obliquely irradiates a substrate medium interface, is totally reflected and generates an evanescent field; 2) the evanescent field is used for illuminating the surface of a sample; a strength image of the surface of the sample is received by a microscope; 3) the strength image is subjected to Fourier transformation; a corresponding frequency spectrum is obtained and is restored; a corresponding frequency spectrum restoring image is obtained; 4) the direction of the incident illumination light is changed for many times around the sample till the direction of the incident illumination light covers 0-360 DEG; frequency spectrum restoring images in different directions are obtained; 5) the frequency spectrum restoring images in the different directions are stacked; a complete high frequency spectrum image is obtained; and 6) the complete high frequency spectrum image is subjected to inverse Fourier transformation; and a super-resolution microscopic image of the observed sample is obtained. The invention further discloses a frequency-shift super-resolution microimaging device based on the evanescent field illumination.

The invention discloses a laser-induced breakdown-pulsed Raman spectroscopy combined system which comprises a pulsed laser, a micro-imaging light path, a micro-imaging device, a Raman signal receiving system, a whole-spectrum LIBS signal receiving system, a spectrograph and a sample platform. The wavelength of laser is changed by adopting a half transparent and half reflecting mirror beam-splitting and frequency-doubling module, and the laser-induced breakdown-pulsed Raman spectrum is switched by using a mechanical photochopper. The invention also discloses a using method of the combined system. With the adoption of a pulsed laser, a set of optical system and a set of spectrograph system, two different types of signals excited by two different wavelengths are acquired, no movable lens assembly is arranged in the optical system, the structure is stable, and the performance is high.

The invention is suitable for the field of microscope imaging, and provides a three-dimensional fluorescence nano microscope imaging method and a three-dimensional fluorescence nano microscope imaging system, and image equipment. The method comprises the following steps of: producing exciting light; converting the exciting light into a laminar exciting beam; making the laminar exciting beam act on a sample; detecting a fluorescent light emitted by a fluorescent label in the sample layer on which the laminar exciting beam acts; transversely positioning to acquire the two-dimensional position of the fluorescent label; axially positioning to acquire the axial position of the fluorescent label; three-dimensionally reconstructing, combining the two-dimensional position with the axial position to acquire a three-dimensional nano resolution image of the sample layer on which the laminar exciting beam acts; axially scanning to acquire the three-dimensional nano resolution images of different sample layers; and acquiring the three-dimensional nano resolution image of the complete sample. Through the three-dimensional fluorescence nano microscope imaging method and the three-dimensional fluorescence nano microscope imaging system, and the image equipment, the high-precision three-dimensional nano microscope imaging is realized by taking the laminar light beam as the exciting light, and combining with axial scanning, and the method, system and equipment are suitable for three-dimensional microscope imaging of the thick sample, such as cells in the biological field and the like, and solve the problems that the positioning accuracy of molecules in the thick sample is low and the sample is difficult to observe.

Disclosed system as an integrated system integrates micro imaging system with high spectrum imaging system, including optical microscope, spectroscope, area array CCD camera and dedicated software of data acquisition and treatment. The disclosed system provides microcosmic spectrogram combining image with spectrum, applicable to clinical medicine, biology, materials science and microelectronics.

The invention relates to a three-dimensional micro-optical imaging device and aims to provide an imaging method of micro-optical scanning chromatography. The method comprise that: a large sample is sliced up layer by layer through precise mechanical processing technique; and the slice is scanned and imaged while performing the mechanical processing so as to acquire a complete faultage image data set of the sample. The system comprises a mechanical processing module, an imaging module and a data acquiring module, wherein the mechanical processing module is used for realizing scanning type slicing on the sample; the imaging module is used for imaging the sample near a cutter edge; and the data acquiring module is used for scanning and recording the imaging of the sample. The three modules coordinate to realize the functions of a micro-optical scanning chromatography imaging device. The imaging device adopts a synchronous mode of slicing and imaging, the automation degree of the whole process is extremely high, the problem that pictures between different layers are difficult to match does not exist, and the acquisition efficiency of the whole data is high.

A super-resolution STED micro imaging method based on a radially polarized vortex beam comprises the steps as follows: a stimulation beam is aligned, and a first parallel beam is obtained; the first parallel beam is converted into a first radially polarized beam; the first radially polarized beam is modulated and focused on a sample of a focal plane, and a focus solid light spot is obtained; a restraint beam is aligned, and a second parallel beam is obtained; the second parallel beam is converted into a second radially polarized beam; the second radially polarized beam is converted into the radially polarized vortex beam; the radially polarized vortex beam is modulated and focused on the sample of the focal plane, and a focus hollow light spot is obtained, wherein the center of the focus hollow spot and the center of the focus solid light spot are overlapped, so that only a middle point located in the middle position and having a size smaller than a diffraction limit emits fluorescence; and a detector detects the fluorescence emitted by the middle point.

In vivo endoscopic methods an apparatuses for implementation of fluorescence and autofluorescence microscopy, with and without the use of exogenous agents, effectively (with resolution sufficient to image nuclei) visualize and categorize various abnormal tissue forms.

The invention relates to a sand-filling type clamp fastener for nuclear magnetic resonance imaging, which belongs to the technical field of scientific research apparatuses for petroleum. The sand-filling type clamp fastener is vertically inserted into a micro-imaging probe of a nuclear magnetic resonance imager, the design pressure is 0-15MPa, and the design temperature is 0-70 DEG C. The sand-filling type clamp fastener can be used for carrying out indoor experiment research on the seepage and transport properties of a multi-phase multi-component fluid in an analog core. Besides a seal ring, all components of the sand-filling type clamp fastener are made from a polyamide-imide material which does not have magnetism, meets the high-temperature high-pressure requirements and has no effect on nuclear magnetic signals. The sand-filling type clamp fastener has the advantages of compact design structure, multiple times of filling, repeatability of use, convenience of operation in an experiment process, simplicity and applicability.

The invention belongs to the technical field of spectral measurement and relates to a high-space-resolution double-shaft confocal atlas micro-imaging method and device. According to a core concept of the method, a double-shaft confocal micro-technique and a spectral detection technique are organically merged, and a normally-forgotten Reyleigh light is utilized to carry out auxiliary detection, so that the space resolution of a system is improved, and three detection modes including three-dimensional tomographic imaging, spectral detection and microcell atlas tomographic imaging are realized. The method and the device have the advantages of high space resolution, accuracy in positioning, high spectral detection sensitivity and the like, have wide application prospects in the fields of biomedicines, physical material science, petrochemical engineering, environmental science and the like and provide new ways for the high-space-resolution detection of microcell three-dimensional geometric positions and spectra.