Confocal micro imaging system using dummy pinhole

Abstract

The invention relates to a confocal imaging system in fields of optical microimaging, it is a confocal microscopic system which uses virtual confocal pinhole to make the system to obtain the longitudinal chromatography capability. It's widely used in the fluorescence microscopy, optical microscopy and so on which possess the microtechnique of three-dimensional imaging capability. The invention includes: light source, collimation lens, beam splitter, microscope objective, objective table, collective lens and CCD. It characterized in that the photosensitive surface of CCD is directly located at the focal plane of the collimation lens, the computer sets the virtual pinhole at the corresponding position of two-dimensional digital image collected by CCD based on the position of the focal point of collimation lens, the signal values of the pixels in the pinhole are accumulated as the signal intensity of current scanning point to eliminate the effect of stray light in non-focal plane to the image quality. The function of virtual confocal pinhole is significant with the physical confocal pinhole and the position, size can be controlled and adjusted by computer, and it possesses the advantages of convenient gauging adjustment.

Description

technical field [0001] The present invention relates to a confocal imaging system in the field of optical microscopic imaging, in particular to a confocal microscopic system that enables the system to obtain longitudinal tomographic capabilities by using a virtual confocal pinhole, which can be widely used in fluorescence microscopy Microscopic techniques with three-dimensional imaging capabilities, such as non-linear optical microscopy and non-linear optical microscopy. Background technique [0002] Because confocal microscopy imaging not only has higher resolution than traditional optical microscopy, but also has unique longitudinal tomographic capabilities, it is widely used in biology, biomedicine, industrial detection, and metrology. Confocal pinholes play a crucial role in the longitudinal tomographic capabilities of confocal microscopy systems. In the system, the measured point and the confocal pinhole are in a conjugate position. Due to the particularity of the posi...

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Problems solved by technology

[0003] However, traditional confocal microscopy systems use a physical confocal pinhole, such as the core diameter of an optical fiber

In the actual practical process, the following problems usually exist in the physical confocal pinhole: the size of the pinhole is generally in the range of microns, and a precise and stable three-dimensional positioning device is required. After precise and cumbersome adjustments, the pinhole is adjusted to the focus position of the collecting lens, otherwise it will easily cause problems such as loss of light energy and resolution reduction; the size of the pinhole cannot be adjusted and changed at will according to the actual practical situation; after a long time, the pinhole and the focus position are prone to deviation. In addition, there are still problems such as pinhole blockage and pinhole cleaning troubles.

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