The present invention discloses a laser pen indicating and light spot trajectory identification method which belongs to the interactive reorganization technical field. Step 1, according to laser pen indicating information, a user obtains video information of a display screen from a video input device; step 2, an embedded device extracts light spots from the acquired video information, and determines the positions of the light spots after a de-noising process; step 3, the position coordinates of the light spots are transmitted to a computer which controls a target screen; step 4, according to the position coordinates of the light spots, the computer which controls the target screen recognizes the light spot trajectory; with the specific light spot information, the trajectory information indicated by the user is obtained; when the recognition is succeeded, step 5 is carried out; step 5, after the trajectory recognition is succeeded, the trajectory of the recognition result of the original trajectory is optimized, so that a new trajectory is obtained; the optimized result is displayed with visually optimized effect on the target screen to replace the indication effect of the step 3. The method can be applied in various interactive recognition display circumstances so as to carry out highly effective light spot analysis and trajectory recognition.