160 results about "Blood cell analysis" patented technology

A sending mode and the sending device automatically for the blood cell analysis instrument are to convert the tube to send the sample. The auto sending sample device includes the tube pushing device, the mixing device, the scan device and puncturing device. The tube pushing device is connected to the mixing device, the scan device and puncturing device. It will combine the mixing equip and scan device into one mixing scan device. The invention makes tube blood mix under the situation that the tube doesn't escape from a tube, and have good mixing efficient. But the blood collecting can be completed under the situation that the tube doesn't escape from a work. So it makes the puncturing sample more convenient and simple. It can further shorten the blood sample entering time, have operation more simple, the equipments are more cleverly made and the manufacturing cost is cheaper and higher automation degree.

The invention belongs to the technical field of blood cell analysis, and particularly relates to a blood cell analysis chip, analysis meter and analysis method. The blood cell analysis chip disclosed by the invention comprises a white blood cell / hemoglobin analysis chip and a red blood cell / blood platelet analysis chip; the white blood cell / hemoglobin analysis chip performs white blood cell classification by adopting the sheath flow technology and the multi-angle laser polarized light scattering technology, and detects the concentration of hemoglobin by adopting the light absorption method; the red blood cell / blood platelet analysis chip counts the red blood cells and blood platelets respectively by adopting the electrical impedance technology and the floating threshold technology. According to the invention, the blood cell analysis chip is utilized to realize classification of blood cells, has the advantages that the structure is simple, the size is small, the cost is low, the operation is convenient, the easy maintenance and transportation are realized, the chip can be discarded after being used, and the like, satisfies the development requirements of miniaturization, integration and portability of an analysis instrument, and is particularly suitable for field detection, emergency analysis, household application and use of primary care departments.

The invention belongs to agents for medical diagnostic apparatuses, and in particular relates to an agent for a blood cell analyzer. The agent for the blood cell analyzer consists of a diluent, an EOI hemolytic agent, an LH hemolytic agent, an EOII staining solution and a cleaning solution. The agent for the blood cell analyzer is wide in range of use, suitable for many types of machines, suitable for detectors with machine types of BC5100 / 5180 / 5300 / 5380 / 5310 / 5390 and the like, and stable and accurate in detection result; the R value of a correlation coefficient after linear regression reaches more than or equal to 0.99 compared with a Mairui original blood cell agent and expresses relatively good relativity; and moreover, the agent is rich in raw material and low in price, and the raw materials are nontoxic and harmless and do not have threats on operators, so that the burden of the environment is lightened, and the raw materials are beneficial for popularization and application of the agent for the blood cell analyzer.

The five classifying whole blood cell analysis method based on visual shape includes analysis in two channels, including one conventional hemacytometry to obtain total number of blood cells, and one image recognizing technological channel to recognize and calculate blood cells of different categories. The results of these two channels are then used in further operation to obtain the numbers of different categories of blood cells. The present invention combines image recognizing technology with conventional hemacytometry, and has high accuracy and high automation.

The invention relates to a hemolytic agent for a blood cell analysis device. The hemolytic agent is capable of cracking the erythrocytes in the blood, and the blood with cracked erythrocytes can be used for hemoglobin concentration determination. In addition, the invention also relates to a leukocyte classification reagent system which comprises the hemolytic agent and an inhibitor. After the hemolytic agent cracks the erythrocytes, the inhibitor can be added into the blood to prevent the hemolytic agent from damaging the morphology and structure of the leucocytes. Furthermore, the invention also relates to a method of classifying the leucocytes by using the reagent system.

The invention discloses a multi-channel micro-fluidic chip for rapid blood self-inspection.The chip comprises a substrate and a cover plate.A sample introduction channel, an inertia separation channel, a flow distribution channel, a plasma analysis channel and a blood cell analysis channel.The sample introduction channel, the inertia separation channel and the flow distribution channel are connected in sequence.The flow distribution cannel is of a Y-shaped structure to be connected to the plasma analysis channel and the blood cell analysis channel.The sample introduction channel is used for inputting blood to be inspected.The inertia separation channel separates blood into plasma and blood cells, and the plasma and the blood cells flow into the flow distribution channel.The flow distribution channel introduces the plasma into the plasma analysis channel through the Y-shaped structure for detecting and analyzing the plasma, and introduces the blood cells into the blood cell analysis channel for detecting and analyzing the blood cells.The highly-integrated multi-channel micro-fluidic chip is adopted, and the function of detecting various kinds of blood is achieved at a time on the basis that carrying is convenient.

A method for indentifying red cell fragments is used for blood cell analysis with cell counting by the resistance method, and comprises the following steps: A. counting white blood cells in a blood sample, drawing a white blood cell column diagram, and recording the full width at half maximum of each particle pulse during the process of particle pulse signal identification; B. locating an area A with possible existence of red cell fragments and an area B without any red cell fragments on the white blood cell column diagram, and determining whether the existence of red cell fragments interfering with white blood cell counting is true in the area A according to the comparison between the distribution of the full width at half maximum of particle pulse in the area A and in the area B; and C. in the case of disturbance caused by red cell fragments, determining the position, in which the interference peak of red cells ends, and correcting the count value of white blood cells according to the ending position of the interference peak of the red cells. The method achieves the effect of improving the accuracy of the result of white blood cell counting and the accuracy of white blood cell classification.

The invention discloses an integrated blood inspection platform and a using method thereof. The integrated blood inspection platform comprises a sample feeding center and inspection instruments, wherein each inspection instrument is fixed on a bracket; the sample feeding center is connected with the inspection instruments through a uniform interface; the sample feeding center comprises a sample feeding arm, a sample rack, a cleaning center, a temperature control system and a reagent rack; the inspection instruments comprise a blood rheology / blood sedimentation instrument, a biochemical analyzer, a blood cell analyzer and a blood coagulation analyzer; the integrated blood inspection platform further comprises inspection modules corresponding to the inspection instruments, wherein the inspection modules are used for controlling the inspection instruments; and the inspection modules respectively comprise a blood rheology / blood sedimentation instrument, a biochemical analysis module, a blood cell analysis module and a blood coagulation analysis module. The inspection devices of various detection methods are integrated into the detection modules with the uniform interface and integrated in the inspection platform, so that the project detection of various detection methods can be realized by using only one inspection platform, the spatial dimensions of the devices are greatly reduced, and the using cost is also reduced.

An apparatus and method of automated blood cell analysis uses technologies from other systems to create a new, robust, improved type of automated microscope, which uses electronic motors and a closed loop control system to minimize ambient factors, such as jarring and temperature changes. Pre-stained blood smear slides are first coated with a thin film of oil and are loaded into a carousel from which they can individually be analyzed. The slides are moved under a low magnification microscope; an optimal area of examination is determined; a focal plane map is calculated for that area, and the positions of white blood cell candidates are computed. The slide is then moved under a high power microscope where a refined focal plane map is computed and the individual white blood cell candidates are imaged. The cells are preclassified and the images are made available for analysis by the technician. Additionally, samples of red blood cells, equivalent to what the technician would do manually, are imaged and presented to the technician for evaluation. The technician may make notes on those cells, both red and white blood cells and archive them.

This invention relates to a blood cell analysis method based on machine vision. Inputting the blood sample into a computer by a camera to convert the color image into the black-and-white and then the binary one and scale the binary image; calculating the area and taking count of the scaled image to obtain the number of RBC, WBC and platelet. This invention has high efficiency and accuracy with low cost.

The invention uses synergetic combination effect of multiple cytoactive protection solution and a stabilizer, and a protective agent provides nutrition components and living environment required by maintaining normal physiological activity for blood cells for anaerobic glycolysis; the stabilizer containing cytolemma and cytoplasm fixed substances with lower concentration can slowly permeate the cytolemma to act on the cytoplasm and firmly combine with biomolecule of cell components such as protein, enzyme, lipid and the like effectively, thus enabling white blood cells, red blood cells and blood platelets to be fixed in different degrees and get effective protection and enabling blood cells to keep apparent morphological characteristics thereof. The blood cells in protection and good stabilized treatment is subjected to assignment according to traceability link delivery procedures and reference methods thereof, and can be used as blood cell analyzer calibrators for clinic usage. The invention uses stem complete blood cell (CBC) from human beings or animals, thus having good stability; the invention can be used in microscope manual counting and cell staining analysis, and has longerexpirydate compared with the current full blood cell calibrators.

The invention supplies a whole blood control material and the manufacture technology that includes blood, anti-assemble agent, fixative and protection agent. The match ratio is 30-80ml / 400ml, fixative 0.5-3.0ml / 400ml, and protection agent 3-15ml / 400ml. The producing method includes the following steps: mixing to equal, gathering healthy people blood, taking safety testing, filtering, mixing 15min taking subpackaging, fixing value and taking verifying to the fixing value, determining the bottle error to gain whole blood control material. The invention has long stationary phase, and simple technology. It is mainly used to verify blood.

The invention relates to a laser system for blood cell analysis, flow cell analysis and body fluid analysis, which comprises a first laser source, a second laser source, a first condensation device, a second condensation device and a front receiving device and a lateral receiving device, wherein the first laser source transmits laser with first wavelength; the second laser source transmits laser with second wavelength; the first condensation device makes the laser transmitted by the first laser source condense and enter a sample room; the second condensation device makes the laser transmitted by the second laser source condense and enter the sample room; and the front receiving device and the lateral receiving device are used for collecting emergent light signals of the sample room. The laser system can reduce the interference of cell membranes on cell nucleus scattering in the process of detecting a cell sample, acquire more information of a detected object, reduce error detection and omission detection of the detected objects, and improve the detection accuracy of a detector.

A blood cell analyzer diluent and a hemolytic agent, are characterized in that one litre diluent is provided with 12.0-4.0g of sodium chloride, 2.0-10.0g of sodium sulfate, 0.8-0.5g of 1,3,2-methylol urea, 0.2-0.5g of copper sulfite, 3.0-8.0g of EDTA-2Na, 0.2-0.7g of Piperacillin Sodium, a borate buffering liquid toning the ph value to 7.2-7.8, and the balance is water; one litre hemolytic agent is provided with 0.8-5.0g of potassium chloride, 0-60.0g of dodecyl trimethyl ammonium chloride, 14.0-0g of octadecyl trimethyl ammonium bromide, 6.0-10.0ml of isopropanol, carbonate or alcaine buffering liquid toning the ph value to 7.2-7.8, and the balance is water. The inventive reagent can form stable hemoglobin derivatives, and the absorption spectrum curves are similar when lambada is 540nm, lambada is 504nm, which can satisfy the clinical inspection requirement; the reagent does not contain cyanide, azide, and has non-toxicity, which can effective improve working atmosphere of operating staff, and can reduce harm of toxicant to personal health.

The invention relates to an end-to-end blood cell recognition model construction method and application. A data sample set is formed based on a full-view image. An artificial intelligence technology is used for training a blood cell recognition model, and a mature recognition model is finally formed through continuous parameter optimization and error analysis optimization of the model.The model input is a single-view blood smear image, and the output is all cell positions, edges and categories on the image. Full-view blood cell analysis is achieved through a computer, interference of human objective factors is greatly reduced, and objectivity and consistency of inspection results are improved. The blood cell recognition model is intelligent, the software algorithm has a self-learning attribute, the training efficiency of the recognition model is gradually improved along with the increase of high-quality labeled images, and the software recognition and classification accuracy can be continuously optimized.

The invention discloses an anti-tumor agent, beverage and food using the agent and a manufacturing method of the anti-tumor agent. The antitumor agent of the present invention exhibits specific effects on human tumor cell A4573 and mouse melanoma B16 cell. In addition, according to the microscopic observation of the live blood cell analysis method, it was found that each erythrocyte in the blood was separated and formed into a perfect circle, and abnormally shaped erythrocytes such as acanthocytes were normalized, and the diameter of white blood cells was more than 2.5 times that of erythrocytes. The anti-tumor agent exhibits multiple immune activities in addition to having immune activities against tumors. Beverages and food containing the antitumor agent can be used as health food and the like. The production method of the anti-tumor agent comprises: heating wheat germ and the like at 110° C. with steam for 3 hours, and then fermenting with microorganisms such as koji at 30° C. for 48 hours.

The invention relates to a blood cell segmentation and recognition model construction method and a blood cell recognition method, and provides a blood cell segmentation model and recognition model construction method on one hand and a cell recognition method based on a blood cell segmentation model and a recognition model on the other hand. The blood image database is generated based on the full-view image, and the blood cell segmentation model is trained, so that the accuracy and comprehensiveness of data are ensured, and the segmentation accuracy of the blood cell segmentation model is improved. Full-view blood cell analysis is realized by utilizing a computer, so that the interference of human objective factors is greatly reduced, and the objectivity and consistency of an inspection result are improved. The blood cell segmentation model and the recognition model are intelligent, the software algorithm has a self-learning attribute, the training efficiency of the recognition model isgradually improved along with the increase of high-quality labeled images, and the software recognition and classification accuracy can be continuously optimized.

The invention belongs to agents for medical diagnostic apparatuses, and in particular relates to a buffer solution for an electrochemiluminescence immunity analyzer and a preparation method of the buffer solution. The buffer solution for the electrochemiluminescence immunity analyzer comprises the following components: potassium dihydrogen phosphate 4, tween-20, dodecyl polyglycol ether, polidocanol, tripropyl amine and citric acid; and the pH value of the buffer solution is 6.5-7.0. The buffer solution for the electrochemiluminescence immunity analyzer is simple to prepare and less in usage amount of tripropyl amine to reduce harms of tripropyl amine to bodies of operators and reduce the burden of the environment; the R value of a correlation coefficient after linear regression reaches more than 0.99 compared with an imported buffer solution for an electrochemical immunity analyzer and expresses relatively good relativity; and moreover, the buffer solution is low in production cost, and the selling price can be reduced by 30% compared with a reagent for an equivalent blood cell analyzer on the market, so that the buffer solution for the electrochemiluminescence immunity analyzer is beneficial for large-scale popularization and application.

The invention provides a green fluorescence cyanine dye, which has a structural general formula I shown in the specification, wherein in the formula, X is C(CH3)2, O, S or Se; R1 and R2 are independently selected form H, C1-18 alkyl groups, OR6, C1-6 alkyl group OR6 or halogen respectively; R3 is N(R5)(R7); R4 is selected from C1-18 alkyl groups, benzyl groups and substituted benzyl groups; the substituted benzyl groups are arbitrarily substituted by the following groups: C1-18 alkyl groups, CN, COOH, NH2, NO2, OH, SH, C1-6 alkoxy groups, C1-6 alkyl amino groups, C1-6 acylamino groups, halogen or C1-6 halogenated alkyl groups; R5 and R7 are independently selected from H or C1-18 alkyl groups respectively; R6 is H or C1-18 alkyl groups; and Y is negative ions. The fluorescence dye can be used for DNA (Deoxyribonucleic Acid) quantitative detection and biological dyeing, and is applied to the fields of nucleic acid marking, blood cell analysis, clinic medical diagnosis, immune analysis detection and the like.

The diluent for hemocyte analyzer includes sodium hydroxide 430-470 g / L, dimethylol urea 0.8-1.2 g / L, phosphoric acid 430-470 ml / L, non-ionic surfactant 0.3-0.7 g / L, procaine hydrochloride 0.09-0.13 g / L, anhydrous sodium sulfate 1.0-9.5 g / L, and sodium chloride 0.3-0.7 g / L except water. The diluent of the present invention may be used in various kinds of hemocyte analyzer.

The invention relates to the technical field of medical and health apparatuses, particularly to a sample introduction device of a blood cell analyzer. The sample introduction device includes a test tube sample holder loading disc, a synchronous belt, synchronous pulleys driving the synchronous belt to do reciprocating motion, a motor driving the synchronous pulleys to rotate and a guide mechanism. The guide mechanism includes linear slide rails parallel to the synchronous belt and a sliding block able to slide on the linear slide rails. The sliding block is in fixed connection with the synchronous belt and is driven by the motor to move. The sample introduction device also includes a push block that is fixed on the sliding block and does reciprocating motion along with the synchronous belt. Under the driving of the push block, the test tube loaded test tube sample holder is conveyed to a specific location along the guide mechanism. The device provided by the invention employs the linear guide rails to replace existing linear bearings to serve as the guide transmission mechanism, the load is large, the stability is stronger, and the rigidity is enough.

The invention discloses a particle counting device. The particle counting device comprises a front pool part, a rear pool part, a counting component arranged between the front pool part and the rear pool part, wherein the rear pool part comprises a rear pool shell, a rear sheath inlet, a capturing pipe, a waste liquid outlet, a rear pool cleaning outlet and a rear pool; the rear pool is formed by an internal cavity of the rear pool shell; the rear sheath inlet is arranged on the rear pool shell and is communicated with the rear pool; one end of the capturing pipe, which is close to the counting component, is provided with a capturing pipe inlet, and the other end of the capturing pipe, which is far away from a technical component, is communicated with the waste liquid outlet; and the rear pool cleaning outlet is arranged at the middle part of the capturing pipe, and is respectively communicated with the rear pool and the waste liquid outlet. The invention also discloses a blood cell analysis device adopting the particle counting device. The particle counting device and blood cell analysis device disclosed by the invention have the advantages of simple structure and high reagent utilization ratio.

The invention provides a high-definition blood cell analysis and detection method. The method is characterized by comprising the following steps: (1) carrying out system initialization, namely removing a blood sample objective table to an initial position, putting a test tube filled with a blood sample into the blood sample objective table, enabling an LED blue light to operate and an objective table intermediate shaft to rotate, and driving the test tube filled with the blood sample to rotate, so that blood in the blood sample test tube is layered and a CCD image sensor starts to operate; and (2) enabling the blood sample objective table to start to enter into a shooting area of the CCD image sensor, displaying an image shot by the CCD image sensor on a liquid crystal display screen, and enabling a white light to start to operate and the blue light to maintain a working state. The method provided by the invention has the advantages that the CCD image sensor is adopted for acquiring a blood image, and image acquisition definition is improved; and three double photoelectric cells are taken as a limit switch, a movement position of the blood sample objective table is controlled, and automatic control of the movement position of the blood sample test tube is realized.

The invention discloses a measurement method and a measurement device capable of performing full-automatic peripheral blood sample injection for blood cell analysis. The measurement device comprises a test tube rack for loading a centrifuge tube, a sample injecting loading platform mechanism for sending the test tube rack into an analyzer, a sampling needle mechanism for spitting and sucking oscillation, an unloading retreating platform mechanism for retreating the test tube rack from the analyzer, an automatic diluent adding button, an automatic counting button and an automatic control system, wherein the automatic control system is connected with the sampling needle mechanism for spitting and sucking oscillation, the sample injecting loading platform mechanism, the unloading exiting platform mechanism, the automatic diluent adding button and the automatic counting button. After adoption of the measurement device and the measurement method, high automation degree and high efficiency can be achieved.

The invention discloses an automatic pipeline for blood cell analysis and POCT immunoassay, and the pipeline comprises a rack, a first conveying platform, a first mixing device, a first sampling mechanism, a first detector with an analyzing device, a first unloading platform, a first conveying device, a second conveying platform, a relay mechanism, a second mixing device, a second sampling mechanism and a fluorescent immunoassay analyzer with a plurality of test channels, wherein the automatic pipeline for blood cell analysis and POCT immunoassay with the above structure organically combines blood cell analysis and fluorescence immunoassay to achieve detection of blood cells and POCT immunization programs, and the plurality of test channels using the fluorescent immunoassay analyzer can freely combine different test items, thereby providing versatility and expandability, shortening sample detection cycle, improving efficiency, reducing manual intervention and reducing error rates.

The invention relates to hemolysin used for blood cell analysis, in particular to the environmental-protection typed hemolysin of no-cyanogen leucocyte three-classification used for blood cell analysis. The hemolysin comprises the components as follows (by density): 0.5-5.0g / L of sodium lauryl sulphate (SLS), 3.0-10.0g / L of quaternary ammonium salt, 0.5-5.0g / L of nonionic surfactant, 0.5-3.0g / L of alkali metal salt, and residual quantity of deionized water. The hemolysin of the invention can quickly dissolve erythrocyte and release haemoglobin; the hemolysin and the haemoglobin form stable haemoglobin derivatives so as to lead the concentration to be exact and easy to be measured; furthermore, the leucocyte three-classification measurement is carried out simultaneously. The hemolysin of the invention can be highly relative to the HiCN mensuration recommended by International Council for Standardization in Hematology (ICSH) and can carry out the leucocyte three-classification exactly and quickly.

The invention discloses a reagent set for a five-classification hematology analyzer. The reagent set comprises a diluent, a hemolytic agent and a staining fluid. The reagent set for the five-classification hematology analyzer has the characteristics of reasonable formula, easily accessible raw material, easiness in processing, low cost and the like, and therefore, has a very good popularization value. The status quo that a regent for a hematology analyzer in China depends on import is changed. As the hemolytic agent-SLS in a reagent is a cyanide-free hemolytic agent, not only are harms to thebody health of personnel of production units and experiment operators greatly reduced, but also environmental pollution caused by a cyanide is avoided. The reagent is suitable for an XN-series five-classification full-automatic hematology analyzer of Sysmex Corporation in Japan, and correlation coefficients of the imported XN reagent and white blood cell, red blood cell, hemoglobin, blood platelet and the like are all more than 0.95.

The invention discloses a method for preparing a quality control product for a five-class blood cell analyzer. The method comprises: step one, preparing fixing liquid; step two, preparing blood cell preservation liquid; step three, carrying out preliminary fixation on whole blood for several hours, carrying out centrifugal separation, fixing red blood cells and blood platelets again for several hours and then carrying out centrifugal separation, washing a PBS buffer solution with the pH of 7.2 for various blood cells and removing the fixing liquid for standby; step four, allocating cell concentration values form first to third levels or from first to fifth levels for each standard; step five, adding the blood cell preservation liquid into the fixed blood cells, carrying out mixing, carrying out sampling detection, and carrying fine adjusting based on the detection result; and step six, after successful detection, carrying out subpackaging, labeling, and sampling and quality checking after packaging. Therefore, the consistency with the clinical test specimen medium and the cell parameter is ensured; the precision of the whole blood cell measurement work of the quality control laboratory in the clinical laboratory is met; and the accuracy change is detected. The quality control product can reach the quality of the similar foreign products.

The invention discloses an automatic blood analysis method and system, a blood cell analyzer and a storage medium, and the method comprises the steps: respectively obtaining microscopic images of a to-be-detected sample when the distances between an objective table and an objective lens are h0-d, h0 and h0 + d, wherein the distance h0 between the objective table and the objective lens is the focusing position of the to-be-detected sample; inputting the microscopic images of the to-be-detected samples corresponding to the distances h0-d, h0 and h0 + d into a trained feature fusion network for feature fusion, and outputting a fusion feature map; inputting the fusion feature map into a trained RPN network and a trained FCN network for frame regression, classification and masking, and outputting an instance segmentation map including a classification result and a mask; and counting the number of to-be-detected targets in the to-be-detected sample according to the classification result andthe mask. According to the invention, the problem of low accuracy of the number of blood cells counted by the cell identification and statistics method based on the optimal focusing microscopic imageis solved.