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Cyanogen-free leucocyte tri-grouping environment protection type haemolysin for blood cell analysis

A blood cell analysis and leukocyte technology, applied in the field of hemolysin, can solve the problem of not being able to accurately perform three-grouping of white blood cells at the same time, and achieve the effect of rapid three-grouping of white blood cells

Inactive Publication Date: 2008-12-24
山东兰桥医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The above-mentioned patents are applicable to the determination of Hb but cannot be accurately classified into three groups of white blood cells at the same time

Method used

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  • Cyanogen-free leucocyte tri-grouping environment protection type haemolysin for blood cell analysis
  • Cyanogen-free leucocyte tri-grouping environment protection type haemolysin for blood cell analysis
  • Cyanogen-free leucocyte tri-grouping environment protection type haemolysin for blood cell analysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Prepare the hemolysin according to the following ratio:

[0030] (1) Sodium lauryl sulfate 1.7g

[0031] (2) Hexadecyltrimethylammonium bromide 5.8g

[0032] (3) Emulsifier OP 0.8g

[0033] (4) Potassium chloride 1.4g

[0034] (5) Deionized water 1000ml

[0035] Mix sodium lauryl sulfate, cetyltrimethylammonium bromide, emulsifier OP, and potassium chloride in the above ratio, add deionized water to 1000ml, stir until uniform at room temperature, and filter with a filter.

[0036] The cyanide-free leukocyte three-group environment-friendly hemolysin for blood cell analysis of the present invention is used for the determination experiment of hemoglobin derivatives with a spectrophotometer, and the results are as follows: figure 1 shown.

[0037] Apply the cyanide-free white blood cell three-group environment-friendly hemolysin for blood cell analysis of the present invention to a blood analyzer:

[0038] (1) Determination of hemoglobin concentration, the result is ...

Embodiment 2

[0044] Prepare the hemolysin according to the following ratio:

[0045] (1) Sodium lauryl sulfate 2.0g

[0046] (2) Tetradecyltrimethylammonium bromide 6.0g

[0047] (3) Triton X-100 1.0g

[0048] (4) Potassium chloride 2.0g

[0049] (5) Deionized water 1000ml

[0050] The preparation method is the same as in Example 1.

[0051] The maximum absorbance of the absorption curve of the hemolysin hemoglobin derivatives for blood cell analysis in the present invention is at 538nm. According to the international standard cyanide methemoglobin (HiCN) assay method, the maximum absorbance is 540nm and the hemoglobin concentration measurement results are compared. The hemolysin fully meets the requirements for the determination of hemoglobin concentration.

[0052] The leukocyte tertiary grouping result using the hemolysin of the present invention is highly correlated with the result of the manual microscopic examination of the leukocyte tertiary grouping, and the hemolysin of the pre...

Embodiment 3

[0054] Prepare the hemolysin according to the following ratio:

[0055] (1) Sodium lauryl sulfate 2.0g

[0056] (2) Dodecyltrimethylammonium bromide 8.0g

[0057] (3) Tween-80 4.0g

[0058] (4) Potassium chloride 0.9g

[0059] (5) Deionized water 1000ml

[0060] The preparation method is the same as in Example 1.

[0061] The maximum absorbance of the absorption curve of the hemolysin hemoglobin derivatives for blood cell analysis in the present invention is at 538nm. According to the international standard cyanide methemoglobin (HiCN) assay method, the maximum absorbance is 540nm and the hemoglobin concentration measurement results are compared. The hemolysin fully meets the requirements for the determination of hemoglobin concentration.

[0062] The leukocyte tertiary grouping result using the hemolysin of the present invention is highly correlated with the result of the manual microscopic examination of the leukocyte tertiary grouping, and the hemolysin of the present ...

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Abstract

The invention relates to hemolysin used for blood cell analysis, in particular to the environmental-protection typed hemolysin of no-cyanogen leucocyte three-classification used for blood cell analysis. The hemolysin comprises the components as follows (by density): 0.5-5.0g / L of sodium lauryl sulphate (SLS), 3.0-10.0g / L of quaternary ammonium salt, 0.5-5.0g / L of nonionic surfactant, 0.5-3.0g / L of alkali metal salt, and residual quantity of deionized water. The hemolysin of the invention can quickly dissolve erythrocyte and release haemoglobin; the hemolysin and the haemoglobin form stable haemoglobin derivatives so as to lead the concentration to be exact and easy to be measured; furthermore, the leucocyte three-classification measurement is carried out simultaneously. The hemolysin of the invention can be highly relative to the HiCN mensuration recommended by International Council for Standardization in Hematology (ICSH) and can carry out the leucocyte three-classification exactly and quickly.

Description

technical field [0001] The invention relates to a hemolysin for blood cell analysis, in particular to a cyanide-free white blood cell three-group environmental-friendly hemolysin for blood cell analysis. Background technique [0002] Differential counting of white blood cells and determination of hemoglobin concentration are important indicators for analyzing human blood in medical diagnosis. There are five types of white blood cells in the blood: lymphocytes, monocytes, neutrophils, eosinophils, and basophils. The latter three are collectively called granulocytes. Therefore, white blood cells are divided into three subsets. Groups are: lymphocytes, monocytes, granulocytes. [0003] Hemolysin is a key reagent for testing hemoglobin content (Hb) and white blood cell count (WBC) or white blood cell classification. It is one of the most commonly used reagents in clinical testing. It is used in blood cell analyzers together with diluents. [0004] Determination of hemoglobin c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N33/48
Inventor 韩作湘张忠房宫奇林王春莲张国栋
Owner 山东兰桥医学科技有限公司
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