Treatment method for biological erythrocyte volume long time stabilization

Abstract

The present invention relates to a treatment method capable of making biological red blood cell volume be stable for a long-period, and is used for preparing quality control material and reference material for blood cell analysis. Said treatment method includes the following steps: (1), stopping or reducing melabolism of red blood cell; (2), phospholipids component treatment for stabilizing red blood cell membrane; and (3), treatment for stabilizing red blood cell membrane protein. The effective component of chemical reagent used in step 2 includes manganic acid, cadmic acid, chromic acid and picric acid or metal salts correspondent to these acids, and the effective component of chemical reagent used in step 3 includes aldehyde material, and the chemical reagents used in the step 1 include the chemical reagents used in step 2 and step 3. Said treatment process can be implemented at room temperature.

Description

technical field [0001] The invention relates to medical preparations, in particular to medical preparations containing biological red blood cells, in particular to preparation of red blood cell-containing quality controls used in blood cell analysis. Background technique [0002] The long-term stability of red blood cell volume has great application value in biological research. Taking the blood cell analyzer as an example, the quality control and calibration of the instrument need to be realized using cells with accurate and stable volumes, and red blood cells are the most abundant component in biological blood, and their influence ability is often 1000 times that of white blood cell particles. is the most critical factor in the control. The blood cell analyzer can analyze multiple information such as the number, volume, and hemoglobin of cells. If the volume parameter test is inaccurate, it will lead to test errors of many related parameters, which will have a great impac...

AI Technical Summary

Problems solved by technology

These technologies have the following defects in the application field of preparing quality control substances: if the fixed red blood cells are too strong, they will easily produce the same properties as white blood cells, especially if they will not be dissolved or incompletely dissolved by surfactants; The stability of red blood cell volume and other parameters is insufficient, and does not meet the long-term stability requirements; red blood cells preserved by preservation technology may have or partially have physiological activity, and the stability of parameters can be maintained in a short period of time, but with the extension of the test time, Changes in the internal and external conditions of red blood cells will cause failure

[0004] It can be seen how to process red blood cells simply and quickly so that it can: maintain the ability to react with the hemolysin in the instrument, and will not become false leukocyte particles to interfere with the determination of other parameters, while maintaining long-term stability of the volume. The average red blood cell volume changes slightly within the validity period required by the calibrator, and the existing technology does not provide a ready-made solution

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