The invention discloses a novel traditional Chinese medicine composition for treating the fatigue syndrome of sub-healthy people and the fatigue, the hypodynamia and the inferior immunologic function of a patient with acquired immune deficiency syndrome and a preparation method thereof. The main components of the traditional Chinese medicine composition comprise the following several raw materials of medicines: crude astragalus root, yam, white peony root, radix codonopsis pilosulae, angelica, solomonseal, ginseng, tuber fleeceflower, American ginseng, antler, aweto, ophiopogon root, Chinese wolfberry and prepared rehmannia root. The medicine composition can be prepared into any common oral preparation according to a conventional traditional Chinese medicine preparation method. The invention can obviously improve sub-health, fatigue syndrome, fatigue and hypodynamia of patients with acquired immune deficiency syndrome, enhances the self immunity and has the advantages of definite curative effect, obvious effect, convenient taking, quick acting, wide suitable crowd, no toxic or side effect and the like.