Method for making fermenting bed for ecological pig breeding by using composite strain culture
A technology of complex strains and ecological pig raising, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problem of short service life of strains in raw materials and fermentation conditions, cumbersome production process operations, and poor quality of strains Stability and other issues, to achieve the effects of shortening the degradation cycle, improving degradation efficiency, and strong bacterial activity
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specific Embodiment approach 2
[0053] 1. Preparation method of composite bacterial strain starter
[0054] Strain culture on inclined plane→liquid first-level seed (shake flask) culture→liquid second-level (seed tank)→fermentation tank culture→high-speed centrifuge→vacuum freeze dryer→crusher→determination of bacteria count→combination of bacteria in proportion→packaging → Finished starter
[0055] Seed culture: Utilize any one of the above-mentioned strains, adopt conventional experimental conditions to cultivate, that is, use carbohydrates as carbon source (said carbohydrates can be glucose, sucrose, lactose, fructose, maltose, starch) One or more of them), peptone, yeast extract or beef extract as nitrogen source, and adding appropriate amount of inorganic salts and trace elements, using deionized water to prepare a solution as a seed medium, and cultured to obtain liquid bacteria Kind, ready to use. Since the cultivation process of the above bacterial strains refers to the cultivation method described...
specific Embodiment approach 3
[0068] 1. Preparation method of composite strain starter
[0069] Strain culture on inclined plane→liquid first-level seed (shake flask) culture→liquid second-level (seed tank)→fermentation tank culture→high-speed centrifuge→vacuum freeze dryer→crusher→determination of bacteria count→combination of bacteria in proportion→packaging → Finished starter
[0070] Seed culture: Utilize any one of the above-mentioned strains, adopt conventional experimental conditions to cultivate, that is, use carbohydrates as carbon source (said carbohydrates can be glucose, sucrose, lactose, fructose, maltose, starch) One or more of them), peptone, yeast extract or beef extract as nitrogen source, and adding appropriate amount of inorganic salts and trace elements, using deionized water to prepare a solution as a seed medium, and cultured to obtain liquid bacteria Kind, ready to use. Since the cultivation process of the above bacterial strains refers to the cultivation method described in the Ch...
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