The invention discloses beef flavor barbecue seasoning and a method for preparing the beef flavor barbecue seasoning. The beef flavor barbecue seasoning is prepared from, by weight, 20-30 parts of fresh beef, 10-20 parts of papaws, 15-20 parts of blueberries, 10-15 parts of mint, 10-15 parts of honeysuckle, 8-10 parts of green tea, 6-8 parts of zingiber mioga, 4-5 parts of spice powder, 2-3 parts of caraway powder, 20-24 parts of ground pepper, 15-18 parts of edible salt, 1-2 parts of radix ophiopogonis, 2-3 parts of north wind grasses, 2-3 parts of jade butterflies, 1-2 parts of genista roots, 1-2 parts of hawthorn, 2-4 parts of barbary wolfberry fruit and 1-2 parts of mango seeds. The beef flavor barbecue seasoning and the method for preparing the beef flavor barbecue seasoning have the advantages that the preparing technology is simple, the ingredients are wide in source, the cost is low, prepared finished products have strong beef flavor and fruit fragrance and are tender in taste, spicy and refreshing and balanced and comprehensive in nutrition, when barbecue food is prepared, the flavor can be greatly improved, peculiar smell can be removed, appetite is effectively promoted, and good spleen-fortifying and stomach-nourishing effects can be achieved.