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Hemolytic agent, leucocyte classification reagent system and classification method

A white blood cell classification and hemolytic agent technology, which is applied in the field of blood cell analysis reagents and analysis, can solve problems such as damage to pipelines and devices, and achieve the effects of avoiding damage, improving safety, and shortening the time required for measurement

Active Publication Date: 2008-08-06
SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the pH value of the first reagent system in the patent is low, which may cause damage to the pipelines and devices of the automatic blood analysis device

Method used

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  • Hemolytic agent, leucocyte classification reagent system and classification method
  • Hemolytic agent, leucocyte classification reagent system and classification method
  • Hemolytic agent, leucocyte classification reagent system and classification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Absorption Spectra of Hemoglobin Derivatives

[0079] The reagent composition of a kind of hemolytic agent is as follows:

[0080] Quaternary ammonium salt surfactant 31551 0.5g / L

[0081] (Henan Daochun Chemical Technology Co., Ltd.)

[0082] Polyoxyethylene cetyl ether 0.1g / L

[0083] EDTA·Na 25mM

[0084] NaCl 8g / L

[0085] Isothiazolinone preservatives 0.05%

[0086] Tris 0.02M

[0087] Amount of HCl to adjust pH to 9

[0088] Among them, the quaternary ammonium salt surfactant 31551 is a mixture in which n is an integer of 3 to 5 in the following structure:

[0089]

[0090] Take 6 μL of blood sample and mix with 1994 μL of the above-mentioned hemolytic agent, and measure its absorption spectrum at 450-700 nm on a Shimadzu-1240 spectrophotometer after 30 seconds. Such as figure 1 As shown, the hemoglobin derivative produced by the hemolytic agent after lysing red blood cells has a maximum absorption peak at around 540nm, and a shoulder peak at around 5...

Embodiment 2

[0094] Determination of hemoglobin concentration

[0095] The reagent composition of a kind of hemolytic agent is as follows:

[0096] Quaternary ammonium salt surfactant 31551 0.1g / L

[0097] (Henan Daochun Chemical Technology Co., Ltd.)

[0098] Polyoxyethylene cetyl ether 0.1g / L

[0099] EDTA·Na 2 5mM

[0100] NaCl 8g / L

[0101] Isothiazolinone preservatives 0.05%

[0102] CAPS 0.02M

[0103] NaOH Amount to adjust pH to 11

[0104] Twelve clinical blood samples were taken, and the hemoglobin concentration was measured on Mindray BC-3000 automatic blood analyzer using the above-mentioned hemolytic agent. At the same time, on the Mindray BC-3000 automatic blood analyzer, the hemoglobin concentration of the above samples was measured in parallel using the original hemolytic agent. The result is as image 3 As shown, the measured value of the hemolytic agent in this example has a good correlation with the measured value of Mindray BC-3000 h...

Embodiment 3

[0106] White blood cell count

[0107] The reagent composition of a kind of hemolytic agent is as follows:

[0108] Quaternary ammonium salt surfactant 31551 0.5g / L

[0109] (Henan Daochun Chemical Technology Co., Ltd.)

[0110] Polyoxyethylene lauryl ether 0.1g / L

[0111] EDTA·Na 2 5mM

[0112] NaCl 8g / L

[0113] Isothiazolinone preservatives 0.05%

[0114] TriS 0.02M

[0115] NaOH Amount to adjust pH to 9

[0116] The composition of an inhibitor reagent is as follows, its pH value is 5:

[0117] Trisodium citrate 0.065M

[0118] Citric acid 0.035M

[0119] NaCl 8g / L

[0120] For 12 clinical blood samples, 20 μL each was mixed with 980 μL of the above-mentioned hemolytic agent, and after 6 seconds, 300 μL of the above-mentioned inhibitor was added to terminate the reaction. Then, white blood cell count was performed on Mindray BC-5500 automatic blood analyzer. At the same time, on the Mindray BC-5500 automatic blood analyzer, the white...

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Abstract

The invention relates to a hemolytic agent for a blood cell analysis device. The hemolytic agent is capable of cracking the erythrocytes in the blood, and the blood with cracked erythrocytes can be used for hemoglobin concentration determination. In addition, the invention also relates to a leukocyte classification reagent system which comprises the hemolytic agent and an inhibitor. After the hemolytic agent cracks the erythrocytes, the inhibitor can be added into the blood to prevent the hemolytic agent from damaging the morphology and structure of the leucocytes. Furthermore, the invention also relates to a method of classifying the leucocytes by using the reagent system.

Description

technical field [0001] The present invention relates to a blood cell analysis reagent and analysis method, in particular to a hemolytic agent which can be used for the determination of hemoglobin concentration, a reagent system for white blood cell classification and a method for using the reagent to classify white blood cells. Background technique [0002] In the field of clinical testing, the determination of hemoglobin concentration and white blood cell classification in patient blood is of great significance in the diagnosis and treatment of various blood diseases. [0003] Both the determination of hemoglobin concentration and the classification of white blood cells on an automatic blood analysis device involve the use of hemolytic agents. On the one hand, the hemolytic agent can dissolve red blood cells and release the hemoglobin in the cells for the determination of hemoglobin. On the other hand, after the hemolytic agent destroys the red blood cells, it can eliminat...

Claims

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Application Information

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IPC IPC(8): G01N33/48G01N33/50G01N33/72
Inventor 匡玉吉徐兵
Owner SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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