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Anti-tumor agent, beverages and foods using the same, and a process for manufacturing the anti-tumor agent

An anti-tumor and reagent technology, applied in the field of anti-tumor reagents, to achieve the effects of enhancing immunity and enhancing leukocyte activity

Inactive Publication Date: 2005-12-21
オトコーポレーション +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, there is no such natural antitumor agent known in the prior art, which can (1) separate red blood cell aggregates that cause cerebral thrombosis, cerebral infarction, and pulmonary thromboembolism into a perfect circle, and (2) short-term Repairing abnormally shaped red blood cells into a normal shape to treat diseases caused by these abnormally shaped red blood cells

Method used

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  • Anti-tumor agent, beverages and foods using the same, and a process for manufacturing the anti-tumor agent
  • Anti-tumor agent, beverages and foods using the same, and a process for manufacturing the anti-tumor agent
  • Anti-tumor agent, beverages and foods using the same, and a process for manufacturing the anti-tumor agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] The mixing ratio of raw materials is 250g of wheat germ, 250g of soybean germ and 200g of rice germ. The mixture was heated with steam at 110°C for 3 hours and then cooled. When the temperature reached 30° C., the resulting mixture was mixed with 250 g of Aspergillus tritici and spread thinly on a flat plate. The pan and mixture were placed in a fermentation chamber and fermented at 30°C for 48 hours. Then it was taken out, the plate was removed, and the mixture was dried at 40° C. for 48 hours to obtain an antitumor agent.

Embodiment 2

[0107] Example 2 - In vitro tumor immunization using mice

[0108] (1) The above-mentioned anti-tumor reagents are made into dry powder and dissolved in phosphate buffer solution. An antitumor agent that was not treated with hot water was thus prepared, and treated with hot water (after boiling) at 90° C. for 20 minutes. Each solution was added to the culture medium of human tumor cell A4573 and mouse melanoma B16 cell in a 96-well plate so that the final concentration was 1 mg / ml. 6 and 24 hours after the addition, a living cell detection reagent (tetrazolium salt: manufactured by Nakarai Tesk) was added, and the color reaction was measured using a microplate analyzer (450 nm) 1 hour later. The same measurement was performed on a control group to which no antitumor agent was added (hereinafter referred to simply as "control group"). And repeat the measurement 4 times.

[0109] The result is as figure 1 and figure 2 shown. It demonstrates that the number of surviving hu...

Embodiment 3

[0117] Example 3 - Microscopic observation according to live blood cell assay

[0118] (1) The blood of an adult woman suffering from collagen disease was collected, and microscopic observation was performed according to the vital blood cell analysis method. It was found that the red blood cells were aggregated in an abnormal shape ( Figure 9 ). Liver function is poor, and acanthocytes (protrusions) are found. Glycolysis becomes poorer and many mold forms are also found. After 30 minutes of administering the described anti-tumor reagent of 1170 mg, blood cell analysis was carried out again, and the results showed that each erythrocyte was separated into a perfect circle, and no acanthocyte (protrusion) was found ( Figure 10 ).

[0119] The above-mentioned dose was continuously administered to the patient for 90 days four times a day, and as a result, the joint pain peculiar to collagen disease disappeared.

[0120] (2) The blood of an adult female with hepatic dysfuncti...

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PUM

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Abstract

The invention discloses an anti-tumor agent, beverage and food using the agent and a manufacturing method of the anti-tumor agent. The antitumor agent of the present invention exhibits specific effects on human tumor cell A4573 and mouse melanoma B16 cell. In addition, according to the microscopic observation of the live blood cell analysis method, it was found that each erythrocyte in the blood was separated and formed into a perfect circle, and abnormally shaped erythrocytes such as acanthocytes were normalized, and the diameter of white blood cells was more than 2.5 times that of erythrocytes. The anti-tumor agent exhibits multiple immune activities in addition to having immune activities against tumors. Beverages and food containing the antitumor agent can be used as health food and the like. The production method of the anti-tumor agent comprises: heating wheat germ and the like at 110° C. with steam for 3 hours, and then fermenting with microorganisms such as koji at 30° C. for 48 hours.

Description

technical field [0001] The present invention relates to antitumor agent, its preparation method and beverage and food containing the agent. More specifically, the present invention relates to an antitumor agent obtained by fermenting wheat germ, rice germ, soybean germ, etc. with microorganisms such as koji, beverages and foods containing the agent, and a method for preparing the antitumor agent. The invention is widely used in health food, disease treatment and the like. Background technique [0002] Conventionally, although there are many immune enhancers of synthetic compounds, natural immune enhancers that have no toxic side effects even if taken for a long time are more preferable. Some natural immune enhancers and antitumor agents are known (see Patent Documents 1 to 3). In addition, the inventors of the present invention have proposed an enzyme-containing food that purifies dirty blood (see Patent Document 4). [0003] Patent Document 1: Japanese Patent Application...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/30A23L2/52A23L2/70A23L2/84A23L7/104A23L33/00A23L33/10A61K35/74A61K35/744A61K36/06A61K36/062A61K36/48A61K36/88A61K36/899A61P1/16A61P3/00A61P3/06A61P7/00A61P15/00A61P17/00A61P19/02A61P35/00A61P37/08A61P43/00
CPCA61K35/747A61K36/06A61K36/48A61K36/88A61K36/899C12P1/00A61P1/16A61P15/00A61P17/00A61P19/02A61P29/00A61P3/00A61P35/00A61P3/06A61P37/08A61P43/00A61P7/00A61K2300/00A61K36/8998
Inventor 杉山刚松尾龙彦二口和之
Owner オトコーポレーション
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