49 results about "Tetrazolium salt" patented technology

The invention discloses a class of anti-tumor compounds containing a triazole heterocyclic structure and an application thereof, belonging to the technical field of medicinal chemistry. The compounds containing the triazole heterocyclic structure introduce quintuple 1,2,3-triazole ring in the form of side chains through the amino condensation, bromination, nitrine substitution, link reaction and other reactions on the basis of naphthalimide parent structure, select tetrazolium salt reduction to test 7721 hepatocellular in the experiment of inhibiting proliferation of tumor cells, and also select sulfonyl rhodamine B protein staining method to test MCF-7 breast cancer cells. The experiment results show that the anti-tumor compounds have wide anti-tumor activity and good inhibition effect on the normal growth of tumor cells derived from different tissues of liver cancer, breast cancer and the like.

The invention relates to the technical field of clinical detection, in particular to a detection reagent for the content of alcohol in saliva. The invention discloses the detection reagent for the content of the alcohol in the saliva, which is characterized in that 1.25 to 0.5U of alcohol dehydrogenase, 0.05 to 0.2mg of coenzyme NAD and 0.025 to 0.2mg of tetrazolium salt are loaded and contained in a solid phase or semi-solid phase carrier with the area of 5.0*5.0mm<2>. The detection reagent for the content of the alcohol in the saliva has high specificity, high sensitivity, and quick reaction, does not contain poisonous substances, can detect orally, is safe and reliable, shows a result without a composite sensor host, can directly contrast readings with a colorimetric card, has better thermal stability, and can be preserved for 2 years at room temperature. The reagent has the advantages of good repetitiveness of determination results, convenient carrying, simple preparation technology, low cost, easy industry promotion and convenient field detection, and can be used for the detection of drunk drivers and taken as a simple tool for postmortem examination of judicial doctors.

A method of producing a protein degradation product is provided, by which a protein (including a peptide) in a sample can be degraded quickly and efficiently. A sample containing a protein is treated with a protease in the presence of the tetrazolium compound to give a protein degradation product. Further, by causing a redox reaction between the glycation site of a glycated protein degradation product obtained by this method and a fructosyl amino acid oxidase, and then determining this redox reaction, it is possible to determine the amount of a glycated protein quickly. As the tetrazolium compound, 2-(4-iodophenyl)-3-(2,4-dinitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt etc. can be used.

The present invention describes methods and kits for determining the level of S-nitrosothiols in biological fluid samples. The method includes separating tissue or cellular matter from the biological fluid sample and then contacting the biological fluid sample with paraformaldehyde in an amount sufficient to fix free thiols thereby essentially eliminating diaphorase activity of the free thiols. The biological fluid sample is then contacted with NADPH and nitro blue tetrazolium (NBT) wherein S-nitrosothiols in the biological fluid sample facilitate the reduction of NBT to NBT formazan or diformazan in the presence of paraformaldehyde. The amount of reduced NBT is measured and the determined levels correlate to the amount of S-nitrosothiols in the biological fluid sample.

The invention relates to the technical field of medical biological detection and provides a cell activity detection method. Color changing of a novel single sulfonic acid tetrazolium salt reagent is utilized to monitor the cell activity in real time. A single sulfonic acid tetrazolium salt detecting system is selected for the testing agent, and the good linear dose relation is taken on the NAD(P)H level and the living cell number. According to the testing agent, through the absorbance value of a single sulfonic acid tetrazolium salt reactant at 450 nm, an EC50 curved line is obtained, and evaluation of the cell activity is conducted. The detecting method is based on the cell high sensitiveness. Single sulfonic acid tetrazolium salt is small in cell toxicity and suitable for a living cell foundation test. The single sulfonic acid tetrazolium salt reagent is mild, and detected cells can be further used for the subsequent test after being rinsed. The single sulfonic acid tetrazolium salt reagent is not prone to reacting with reducing substances, and interference is small. The reagent is wide in application range and is suitable for detection after environmental microorganism enrichment, detection of the activity of algae, fungi and the animal and plant cells and detection of the cells and tissue lesion.

The invention discloses a method for fast screening a xanthine oxidase inhibitor or a superoxide anion scavenging agent by utilizing a coupling technique of thin-layer chromatography and bioautography. According to the method, the thin-layer chromatography is combined with biological activity assay, a superoxide anion free radical (O2<.->) generated by an enzymatic reaction reacts with nitroblue tetrazolium (NBT) to generate formazan which is a bluish violet insoluble substance, and substances capable of inhibiting xanthine oxidase or scavenging superoxide anion inhibit the reaction so yellow and / or white spots under a purple background show on a thin layer plate. The method is suitable for analyzing the in-vitro inhibition activity to the the xanthine oxidase or the scavenging activity to the superoxide anion of natural drugs and / or traditional Chinese medicine extracts or monomeric compounds.

The present invention discloses a biosensor, a biostrip and a manufacture method of determination of uric acid by a non-enzymatic reagent, and particularly to the applications of determination of uric acid by a non-enzymatic reagent, which is useful for the quantification of the uric acid levels in the samples. The biosensor of the invention comprises at least a sensor, a biostrip and a non-enzymatic reagent for the determination of uric acid, wherein the biostrip has at least two working electrodes and at least one reaction zone and the non-enzymatic reagent for the determination of uric acid is disposed in the reaction zone on the biostrip, and has at least a tetrazolium salt and an active electron mediator, wherein the reaction zone is used for a sample to initiate the oxidation-reduction reaction of the tetrazolium salt and then, according to the oxidation-reduction reaction, with the active electron mediator and the two working electrodes, an electronic signal is transmitted to the sensor to produce a corresponding micro-current intensity which is in turn calculated by the sensor to reflect the uric acid level in the sample.

A method of conveniently and quickly quantitating subject substances which comprises using as reaction reagents at least dehydrogenases specific respectively to branched amino acids containing D-galactose and L-leucine and phenylalanine, which are the substances contained in biological samples to be assayed, coenzymes, an electron mediator and a tetrazolium salt and detecting formazan, which has been finally formed depending on the concentrations of the subject substances by the enzymatic reactions and oxidation reactions of the biological samples with the reaction reagents, by using both or one of an optical detection procedure and an electrochemical detection procedure, thereby embodying a convenient and quick examination of three diseases with inborn errors of metabolism, i.e., galactosemia, maple syrup urine disease and phenylketonuria.

A method of detecting increased levels of DNA single strand breaks in a eukaryotic cell sample, comprising the steps of: (a) contacting a eukaryotic cell sample to a water-soluble tetrazolium salt under conditions in which said tetrazolium salt is converted to a formazan dye in said cell sample in the presence of NADH or NADPH; and then (b) detecting the presence of the formazan dye in said cell sample, with decreased levels of the formazan dye indicating increased levels of DNA single strand breaks in the eukaryotic cell sample.

The invention discloses a biosensor device, a biosensing test piece of a non-enzymatic type uric acid reagent and production method thereof, which is applicable to the quantitative detection of the concentration of uric acid in body fluid. The biosensor device at least comprises one sensor, one biosensing test piece and one non-enzymatic type uric acid reagent, wherein, the biosensing test piece is at least provided with two active electrodes and one reaction region; the non-enzymatic type uric acid reagent can be arranged in the reaction region of the biosensing test piece and at least provided with tetrazolium salts and active electronic intermedia; wherein, the reaction region is used for leading the body fluid to start the redox reaction of the tetrazolium salts, then an electronic signal is transferred to the sensor by the active electronic intermedia via the two active electrodes according to the redox reaction, and a microcurrent strength value is generated correspondingly, thereby the concentration of the uric acid in the body fluid is calculated and reflected by the sensor.

This invention provides a method for treating cancer in mammals through cellular-organelle-crystallization-induced-death (herein defined as “Cocid”), a method for treating cancer using cellular organelle and / or cytoskeleton crystallizing agents (e.g. tetrazolium salts and their derivatives), pharmaceutical compositions containing a therapeutically effective amount of organelle and / or cytoskeleton crystallizing agents, and compositions containing organelle and / or cytoskeleton crystallizing agents in combination with a pharmaceutically acceptable carrier, diluent or excipient. The crystallizing agents with or without a pharmaceutically acceptable carrier, diluent or excipient, are used in combination with surgery and / or non-surgical anti-tumor treatments.

A method for reducing a tetrazolium salt.

This invention provides a method for treating cancer in mammals through cellular-organelle-crystallization-induced-death (herein defined as “Cocid”), a method for treating cancer using cellular organelle and / or cytoskeleton crystallizing agents (e.g. tetrazolium salts and their derivatives), pharmaceutical compositions containing a therapeutically effective amount of organelle and / or cytoskeleton crystallizing agents, and compositions containing organelle and / or cytoskeleton crystallizing agents in combination with a pharmaceutically acceptable carrier, diluent or excipient. The crystallizing agents with or without a pharmaceutically acceptable carrier, diluent or excipient, are used in combination with surgery and / or non-surgical anti-tumor treatments.

The invention provides a method for determining the total number of viable bacteria based on a WST-8 chromogenic reaction. The method comprises the following steps: mixing a resuspended to-be-detected bacterial suspension with a chromogenic substrate solution to react for 0.5-4 hours to obtain a to-be-detected reaction solution; and determining the absorbance value of the reaction liquid to obtain the total number of viable bacteria. The chromogenic substrate solution is prepared from 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfo phenyl)-2H-tetrazolium salt, an electron carrier, namely, 1-methoxy-5-methylphenazine methyl sulfate, and a bacterial growth nutrient substance. According to the method, the chromogenic reaction of WST-8 is used for detecting the viable bacteria for the first time, and the content of dehydrogenase in bacterial cells can be effectively increased due to existence of bacterial growth nutrient substances in the chromogenic substrate solution, so that the sensitivity of bacterial detection is improved. The method has the characteristics of convenience in operation, rapidness, high sensitivity and wide bacterial detection range.

The invention discloses a preparation method of a kind of new high-energy density materials bis(1-alkyl-5-aminotetrazolium)dodecahydrododecaborate or bis(2-alkyl-5-aminotetrazolium)dodecahydrododecaborate and bis(5-amino-1,4-dialkyltetrazolium)dodecahydrododecaborate or bis(5-amino-1,3-dialkyltetrazolium)dodecahydrododecaborate as well as pure products without crystal water. The preparation methodmainly comprises a metathesis reaction between dodecahydrododecaborate and 1-alkyl-5-aminotetrazolium salt and 2-alkyl-5-aminotetrazolium salt or 5-amino-1,4-dialkyltetrazolium salt and 5-amino-1,3-dialkyltetrazolium salt in a suitable medium. The preparation method has the characteristics of simple program, routine operation, treatment convenience and easy purification.

The invention provides a biochemical method for visualizing cellular organelles (such as centrosome) and / or cytoskeletons (such as microtubules) through the use of crystallizing agents (such as tetrazolium salts), a kit containing crystallizing agents adapted for such uses, and to methods particularly for detecting neoplastic cells in a tissue sample, suspension, or a fluid sample by examining the cells for abnormalities of cellular organelles (such as centrosome) and / or cytoskeletons (such as microtubules) in the number and shapes.

The invention discloses a pressure steam sterilization chemical indicator and a preparation method thereof, belonging to the field of moist heat sterilization indication. The chemical indicator mainly includes two parts: chemical indicator ink and substrate. The weight composition of the active ingredients of the pressure steam sterilization chemical indicator is: 1-10 parts of tetrazolium salt, 5-20 parts of system stabilizer, 50-70 parts of solvent, 10-20 parts of binder, and 5 parts of alkaline auxiliary. ‑20 servings, and 1‑10 servings of antioxidants. The above active ingredients are mixed and stirred to obtain a uniform and viscous composition, which is then sent to a grinder for grinding to form a uniformly dispersed indicator ink. The indicator ink is printed on the substrate by flexographic printing, and after drying, the chemical indicator is obtained by pressure steam sterilization. The pressure steam sterilization chemical indicator provided by the present invention is exposed to the saturated pressure steam conditions of 121 ° C, 132 ° C and 134 ° C, and the color of the indicating ink changes from colorless to dark, which can be determined by the color depth of color development. Determine whether the sterilization is qualified.

The present invention describes methods and kits for determining the level of S-nitrosothiols in biological fluid samples. The method includes separating tissue or cellular matter from the biological fluid sample and then contacting the biological fluid sample with paraformaldehyde in an amount sufficient to fix free thiols thereby essentially eliminating diaphorase activity of the free thiols. The biological fluid sample is then contacted with NADPH and nitro blue tetrazolium (NBT) wherein S-nitrosothiols in the biological fluid sample facilitate the reduction of NBT to NBT formazan or diformazan in the presence of paraformaldehyde. The amount of reduced NBT is measured and the determined levels correlate to the amount of S-nitrosothiols in the biological fluid sample.

This invention provides a quantitative assay technique for soft agar colony formation that can be carried out rapidly with high accuracy. Specifically, this invention relates to a method for evaluation of cell survival comprising: a step of overlaying agar at the bottom of a vessel with agar containing cells, overlaying the agar containing cells with a medium and culturing the cells; a step of removing the medium, adding tetrazolium that produces water-soluble formazan and an electron carrier and culturing the cells; and a step of evaluating cell survival based on the color developed by the water-soluble formazan.

A class of compounds of monosulfonic phenyltetrazole, with the structure of 2-(R1 phenyl)-5 (2-sulfonic phenyl)-2H-tetrazole. The 2-sulfonic phenyl tetrazolium salt of this invention has advantages of low toxicity, short synthetic route, easy control of purity and quality. As the 2-sulfonic phenyl tetrazolium salts has almost no absorption at 450 nm where the reduzate has greater absorption, spectrophotometry can simply and rapidly determine the activity of glutamate dehydrogenase, or the content of NADH / NADPH.

It is an object of this invention to provide a simple measurement method capable of detecting indoxyl sulfuric acid in a sample rapidly and at high sensitivity. By causing sulfatase and tetrazolium salt to act on indoxyl sulfuric acid in a sample to generate a formazan dye, and then calculating the generation amount of the formazan dye, indoxyl sulfuric acid in the sample can be measured more simply, more rapidly, and at higher sensitivity as compared with former methods.

The invention relates to a preparation method of a biochemical detection reagent of chlorinated tetrazolium salts. In the preparation method, formazan is used as a reactant and is oxidized into a chlorinated tetrazolium salt by adopting a hydrogen chloride / hydrogen peroxide system. The preparation method specifically comprises the following steps of: 1) dissolving formazan into the concentrated hydrochloric acid / methanol mixed liquid, 2) dropwise adding aqueous hydrogen peroxide solution under the condition that the dropwise adding temperature range is minus 10 DEG C to 15 DEG C, and stirring at the room temperature after dropwise adding, and 3) removing methanol by rotary evaporation, extracting with water-insoluble organic solvent and drying, adding anhydrous ethyl ether to separate out a crude product, and recrystallizing the mixed solvent to obtain the chlorinated tetrazolium salt refined product. The preparation method provided by the invention has the advantages of improving the yield at last, being simple in treatment, having no heavy metal residual in the product and being green in the preparation process.

The invention discloses a reagent for fast identifying Wen county iron stick yam and an identification method thereof. The reagent is a glucose identification reagent; the identification reagent and glucose take chromogenic reaction. The identification reagent is alkaline buffer liquid containing water-soluble colorless tetrazolium salt. The water-soluble colorless tetrazolium salt is one of materials from red tetrazolium, blue tetrazolium, tetrazolium violet and neotetrazolium chloride. According to the characteristic that the content of glucose and fructose in the Wen county iron stick yam is small, the identification reagent capable of taking chromogenic reaction with glucose is used for identification; if the color development occurs, the result shows that the iron stick yam to be tested is not the Wen county iron stick yam; if the color development does not occur, the result shows that the iron stick yam to be tested is the Wen county iron stick yam. Compared with the prior art, the reagent and the identification method have the advantages that whether the yam is the Wen county iron stick yam or not can be fast identified; the operation is simple.

The present invention discloses a biosensor, a biostrip and a manufacture method of determination of uric acid by a non-enzymatic reagent, and particularly to the applications of determination of uric acid by a non-enzymatic reagent, which is useful for the quantification of the uric acid levels in the samples. The biosensor of the invention comprises at least a sensor, a biostrip and a non-enzymatic reagent for the determination of uric acid, wherein the biostrip has at least two working electrodes and at least one reaction zone and the non-enzymatic reagent for the determination of uric acid is disposed in the reaction zone on the biostrip, and has at least a tetrazolium salt and an active electron mediator, wherein the reaction zone is used for a sample to initiate the oxidation-reduction reaction of the tetrazolium salt and then, according to the oxidation-reduction reaction, with the active electron mediator and the two working electrodes, an electronic signal is transmitted to the sensor to produce a corresponding micro-current intensity which is in turn calculated by the sensor to reflect the uric acid level in the sample.

The invention discloses a class of anti-tumor compounds containing a triazole heterocyclic structure and an application thereof, belonging to the technical field of medicinal chemistry. The compounds containing the triazole heterocyclic structure introduce quintuple 1,2,3-triazole ring in the form of side chains through the amino condensation, bromination, nitrine substitution, link reaction and other reactions on the basis of naphthalimide parent structure, select tetrazolium salt reduction to test 7721 hepatocellular in the experiment of inhibiting proliferation of tumor cells, and also select sulfonyl rhodamine B protein staining method to test MCF-7 breast cancer cells. The experiment results show that the anti-tumor compounds have wide anti-tumor activity and good inhibition effect on the normal growth of tumor cells derived from different tissues of liver cancer, breast cancer and the like.

The present invention relates to a method of visualizing biomolecules, having the steps of: a) providing a sample of immobilized biomolecules in a matrix and carry on the electrophoresis process; b) incubating the matrix of step a) in a solution containing a cyanine-derived molecule, for a time of 5 to 60 minutes, at room temperature, in a container preventing exposure to light, shaking the container at less of 75 rpm; c) transferring the matrix from step b) to a container with a solution having: at least one tetrazolium salt and incubating for a time of 15 to 120 minutes at room temperature under light exposure; d) removing the matrix with immobilized biomolecules from the previous step and washing with distilled water; and e) visualizing directly by the naked eye the biomolecules immobilized in the matrix.

The invention discloses a low-temperature fast preparation method of a perovskite luminous material of formamidine lead bromide nanometer crystals. According to the method, firstly, the raw materials required for preparation are poured into a flask, an ultrasonic cell disruptor is used for stirring the solution for making the materials into a nanometer crystal raw solution; then, acetonitrile and toluene are added; centrifugation is performed; supernatant fluid is removed; precipitates obtained after the centrifugation treatment are taken; then, the toluene is added into the precipitates so that the precipitates are dissolved; then, centrifugation is performed; supernatant fluid is removed; the precipitates obtained after the centrifugation treatment are taken; the FAPbX3 green light nanometer crystals are finally obtained. The FAPbX3 nanometer crystals prepared by using the process provided by the invention can realize stable photoluminescence being 530 to 535 nm; no inert gas and no high-temperature reaction condition are needed in the preparation process; the simple, efficient, low-temperature and great-batch production of the perovskite luminous material can be realized; the quantum yield reaches 80 percent or higher.

The embodiment of the invention belongs to the field of medical examination and determination, and relates to a kit for determining activity of creatine kinase isoenzyme. The kit comprises a first reagent and a second reagent; the first reagent comprises a first buffer solution, adenosine diphosphate, disodium ethylene diamine tetraacetate, magnesium acetate, an activator, adenosine monophosphate, an anti-human CK-MM antibody, AP5A, glucose, NAD (P) + and a first preservative; and the second reagent comprises the following components: a second buffer solution, phosphocreatine, magnesium acetate, hexokinase, glucose-6-phosphate dehydrogenase, tetrazolium salt, diaphorase and a second preservative. The invention also relates to a determination method of creatine kinase isoenzyme activity. According to the technical scheme, the test sensitivity can be improved.