227 results about "Moist heat sterilization" patented technology

The invention discloses a manufacture method of flavor fish cake taking fish and pork as main raw materials. The method comprises the following steps: firstly, removing fishbone of a fresh fish, removing skin, washing the fish, performing fish separating, and mincing or blending the fish, so as to form minced fillet, and performing refined filtration on the minced fillet, so as to obtain refined minced fillet; secondly, performing boning on pork, picking out the skin, mixing fresh ginger into the pork, and mincing the pork, so as to form meat paste; thirdly, performing batching and seasoning on the minced fillet and the meat paste, and uniformly mixing the minced fillet and the meat paste, so as to obtain meat mash; fourthly, putting the meat mash into a high-pressure moist heat sterilizer to perform sterilization on the meat mash; fifthly, performing aseptic subpackage on the sterilized meat mash according to a conventional method, so as to obtain finished fish cake products. The method has the advantages that formula adjustment is performed on the basis of traditional fish cakes, the modern high-pressure moist heat sterilization process is adopted to replace the traditional steaming process, the advanced aseptic subpackage technology is adopted, the formula is reasonable, the process is advanced, the manufactured fish cakes are full of elasticity and toughness, not too cohere, not easy to get loose and not greasy and are nutrient-rich, the retention period can reach more than six months, the product quality is stable, and the method is applicable to industrial production.

A Lucid Ganoderma cultivation bag material comprises, by weight percent, 25-35% of beanstalk crumbs, 20-30% of soybean dregs, 33-53% of sawdust, 2% of land plaster, and soybean whey which is 1-1.2 times of the cultivation bag material in weight. A method for cultivating Lucid Ganoderma by the Lucid Ganoderma cultivation bag material includes firstly, performing moist heat sterilization to the Lucid Ganoderma cultivation bag material at high temperature and high pressure, cooling, and inoculating a Lucid Ganoderma strain; secondly, cultivating with mycelia for 35-50 days to sprout; and thirdly, continuing to cultivate for 30 days, and sprouting and controlling to obtain ripe Lucid Ganoderma fruiting bodies. The soybean dregs contain sufficient phosphorus and vitamin, and the pH value, variation with time, of the soybean dregs fits with required environment for growth of Lucid Ganoderma. By the Lucid Ganoderma cultivation bag material, high-quality and high-yield Lucid Ganoderma fruiting bodies can grow, waste utilization of the beanstalk, the soybean dregs and the soybean whey is achieved, and primary chemical and medicinal compositions of the cultivated Lucid Ganoderma and content of Lucid Ganoderma triterpenes are higher than those of marketable Lucid Ganoderma.

The invention relates to a biological organic foliar fertilizer and a preparation method thereof. The biological organic foliar fertilizer is obtained by adding 50 parts weight of deep well water in 50 parts by weight of waste such as soybean residues, crushed rapeseed meal and the like; mixing the above materials after moist heat sterilization; inoculating compound microbial strains; fermenting for 30 days at a constant temperature; removing fermentation residues, taking the fermentation broth for use; putting 48 parts of the fermentation broth, 15 parts of fulvic acid, 5 parts of magnesium element, 5 parts of manganese element, 5 parts of ferrum element, 5 parts of zinc element, 2 parts of copper element, 2 parts of molybdenum element, 3 parts of a surfactant, 2 parts of PCPA , 2 parts of NAA and 6 parts of sterile water in a reaction kettle; mixing the above materials uniformly; heating to a temperature of 45 DEC C; keeping the temperature for 48 hours for reaction; and adjusting a pH value to 3-4. The biological organic foliar fertilizer can promote crop photosynthesis, activate activities of various enzymes to promote the growth, increase disease prevention and antibiosis of plants and increase resistance of the plants. The biological organic foliar fertilizer is pollution-free and harmless, has durable fertilizer efficiency, can strengthen seedlings, prevent diseases, improve soil and increase production, has no residues after use, improves quality of agricultural products, makes products appear on the market 7-15 days earlier, and increases agricultural economy benefits.

The invention discloses a method for producing a golden flower dark tea by employing rapid fermentation of a raw dark tea brick. The method comprises the following steps: (1) sterilizing in a fermentation room, sterilizing an air-tight fertilization room with an air inlet and an air outlet; (2) sterilizing the dark tea, carrying out moist heat sterilization on a raw dark brick tea in an autoclaved sterilization pot; (3) artificially inoculating an eurotium cristatum strain and blending to prepare a strain solution, cooling the sterilized raw dark tea brick to a room temperature, and soaking in the strain solution or evenly spraying the strain solution on the surface of the tea brick; and (4) uniformly and vertically putting the inoculated tea brick on a fermentation frame of a sterilized fermentation room, fermenting for 7-21 days, taking out and carrying out reduced pressure drying, so as to obtain the golden flower dark brick tea brick. According to the method disclosed by the invention, efficient flowering of the dark tea is achieved; the cost is low; each gram of dry tea contains not more than 1.8*10<7> eurotium cristatum in average; the soup brewed by the golden flower dark tea is clear, and fragrant in aroma; the produced brick tea is stable in quality, can be stored for a long period of time, and has relatively good nutrients and relatively high collection value.

The invention discloses a polluted water body petroleum adsorbent and a preparation method thereof. The preparation method comprises the following steps of: (1) cleaning maize straw, and drying to constant weight; (2) smashing the maize straw, sieving with a sieve of 20-40 meshes to obtain maize straw powder; (3) performing high-pressure moist heat sterilization on the maize straw powder, uniformly dripping an environment-friendly Trichoderma viride Persoon:Fries suspension and a Mandel nutrient fluid, and performing solid-state fermentation culturing under the condition that the temperature is 20-45 DEG C; and (4) culturing for 2-14 days, terminating a reaction, cleaning modified maize straw, and drying to obtain a petroleum adsorbent. A process adopted in the method is simple, the prepared adsorbent has high oil absorption capability and oil preserving capability, an operating process is simple, secondary pollution is avoided, and procedures from production to use are environment-friendly.

The invention relates to an innocent treatment device for medicinal wastes, which comprises a sterilizer, wherein the sterilizer is arranged vertically and comprises a barrel and an upper door and a lower door which are hermetically connected with the barrel, the upper door and the lower door are provided with a drive mechanism, and the bottom of the barrel is provided with a plate turning mechanism. A moist heat sterilization form is adopted in the device, but the treatment method has great breakthrough and reformation; and the device realizes all processes of full-automatic feeding, treatment, discharging, lifting, crushing, cleaning and sterilization of a circulation box, stench treatment, effective ingredient reclamation and the like, achieves 'zero contact' between persons and wastes in the treatment process, reduces the possibility of cross infection, reduces the labor intensity, meanwhile, changes wastes into valuable, and realizes 'innocent' and 'recycle'.

The invention discloses a method for screening an alternaria alternata resistant germplasm material from a petri dish. The method comprises the following steps of: a, culturing alternaria alternata on an agar culture medium; b, carrying out shaking culture of the alternaria alternata in a Czapek culture solution; c, coarsely extracting alternaria alternata toxin; d, determining activity of the toxin by using a biological assay method; e, adding water for diluting a toxin solution and preparing a culture medium; f, carrying out 121-DEG C moist heat sterilization on a toxin-containing culture medium for 15-30min; g, disinfecting tobacco seeds; h, culturing the tobacco seeds on the toxin-containing culture medium; and I, identifying the alternaria alternata resistant tobacco germplasm material according to the condition whether a tobacco shoot root grows in the culture medium. According to the invention, the toxin-containing culture medium is prepared by using the toxin generated by the alternaria alternata, the tobacco seeds are cultured on the culture medium, and the alternaria alternata resistant germplasm material is directly screened from the tobacco seeds rapidly, economically, conveniently and reliably. The method can replace field test screening and can be used for screening the alternaria alternata resistant germplasm material in the petri dish.

The invention discloses a method for producing metabiotics by damp-heat quenching of semi-immobilized probiotics, and the method comprises the following steps: culturing lactic acid bacteria strains through an MRS culture medium to obtain lactic acid bacteria fermentation liquor; concentrating the lactic acid bacteria fermentation liquid until the concentration of the bacterial liquid is 1 * 10 < 11 > cfu / g; mixing the lactic acid bacteria concentrated solution with the soybean fiber powder to obtain a mixture; carrying out inactivation treatment on the mixture by adopting a steam moist heat sterilization method to obtain an inactivated mixture; rapidly cooling the inactivated mixture, so that the temperature of the mixture is reduced to normal temperature within 1-3 minutes; and drying the cooled and inactivated mixture until the water content is 8-10%. According to the invention, the metagen product with a probiotic function and a long shelf life can be produced. Meanwhile, through the damp-heat quenching and rapid cooling technology, thallus splitting decomposition and denaturation can be reduced, and the integrity of thalli is kept. In addition, the wet base is high in thermal conductivity, low in later drying cost, loose and convenient to process.

The invention relates to a preparation method of glycerin and fructose injection, comprising the following steps: adopting a buffer solution to regulate the pH value of glycerol and fructose aqueous solution to be 4.0 to 5.5, carrying out moist heat sterilization at the temperature of 115 DEG C for 30 minutes or at the temperature of 121 DEG C for 10 minutes after filling, and then cooling. The pHvalue of the glycerin and fructose injection prepared by the preparation method is stable, and the content of 5-HMF is low. The invention adopts the sterilization process of a quick cooling sterilizer, thereby shortening the heating time during the sterilization process and reducing the degradation of fructose.

The invention relates to a preparation method of protein feed. The preparation method comprises the following steps: performing moist heat sterilization on fermentation raw materials, cooling for later use, inoculating 10% of mixed strain liquid of aspergillus niger and candida utilis into the sterilized fermentation raw materials, and performing solid-state fermentation at 30 DEG C for 120 hours to obtain a fermentation material; drying the fermentation material and grinding to obtain the protein feed. The prepared feed has relatively high nutritional values; especially the single-cell protein content is remarkably increased; in addition, the feed is low in cost, pollution-free and favorable for increasing comprehensive utilization degree of citruses, and provides a feasible way for converting renewable biological resources into single cell protein and adding feed varieties.

Single cohesiveness or dispersivity cannot simultaneously satisfy different needs in the whole cataract surgery. Chitosan and sodium hyaluronate respectively belong to natural polycation and polyanion polysaccharides. Through charge interaction between chitosan and sodium hyaluronate, a polyelectrolyte complex is formed and the polyelectrolyte complex is processed to form the viscoelastic agent with cohesiveness and dispersivity. Medical chitosan gel has thermolability and after moist heat sterilization, the molecular weight of the chitosan gel is reduced and dispersivity is improved so that the chitosan gel can prevent corneal tissue mechanical damage. After positive and negative charge interaction, ionic crosslinked compound gel still has good cohesiveness, still has typical non-Newton rheological behavior and can be removed easily and reduce residual in eyes. Cohesiveness and dispersivity guarantees that a single product can satisfy demands in different stages in the whole cataract surgery and has a wide clinical application prospect.

The invention provides a tunnel type clothes cleaning cabinet which comprises a ventilation system, a cabinet body and a control system, wherein the cabinet body comprises a ventilation drying box anda moist heat sterilizing box; the moist heat sterilizing box is arranged behind the ventilation drying box, and the two boxes are separated by a rubber soft screen; a clothes hanging rod is connectedwith the two boxes through a sliding rod; and a manipulator is arranged on the sliding rod. In the process of ventilation, the hanging rod is positioned in the ventilation drying box; in the processof moist heat sterilization, the hanging rod is pushed into the moist heat sterilizing box along the sliding rod through the manipulator; and finally, the hanging rod is pushed into the ventilation drying box by the manipulator for drying. In the process of moist heat sterilization, the ventilation system in the ventilation drying box is operated without interruption. The invention has the effectsthat the movable tunnel type clothes cleaning cabinet is designed, clothes can be pushed into different boxes in different stages through the manipulator, and in the process of moist heat sterilization of the cabinet body, the ventilation system in the ventilation drying box is operated without interruption, thus the overflow of hot gas in the process of moist heat sterilization can be reduced.

The invention discloses a fat emulsion injection containing nimodipine and a preparation method thereof. The fat emulsion injection containing the nimodipine is prepared from nimodipine, injection oil, injection phospholipid, injection polyethylene glycol hydroxy stearate, an isoosmotic adjusting agent, a pH regulator and injection water. The preparation method comprises the following steps: mixing the nimodipine, the injection oil, the injection phospholipid with the injection polyethylene glycol hydroxy stearate, and agitating to clear and transparent as an oil phase; evenly mixing the isoosmotic adjusting agent with the injection water as a water phase; mixing the oil phase with the water phase; processing emulsion particles into the stated particle size range by adopting a two-step homogenization method; bulking and sterilizing after adjusting the pH value. The injection phospholipid and the polyethylene glycol hydroxy stearate are adopted as a mixed emulsifier, and the prepared fat emulsion injection containing the nimodipine can endure high-pressure moist heat sterilization, and does not need an expensive sterile manufacturing process, so that the production cost is greatly reduced, and industrial production is facilitated.

The invention belongs to the technical field of biology and relates to a method for producing a micro-ecological preparation through cooperatively fermenting kitchen wastes by utilizing feeding lipase, yeast and lactic acid bacteria. The method specifically comprises the following steps: after the kitchen wastes are crushed and are subjected to moist heat sterilization at a high temperature, adding the feeding lipase, the yeast and a lactic acid bacteria liquid bacteria agent, fully mixing, mixing the mixture with a protective agent after aerobic fermentation and anaerobic fermentation and obtaining the micro-ecological preparation after drying. The method has the advantages that the feeding lipase can efficiently decompose oil and fat in the kitchen wastes, the yeast and the lactic acid bacteria have a good cooperative effect through co-culture, and all nutritional ingredients in the kitchen wastes can be fully utilized under the combined action of the lipase, the yeast and the lactic acid bacteria, so that the micro-ecological preparation with high live bacteria quantity is obtained.

The invention relates to a traditional Chinese medicine preparation-Liuwei Anxiao Pills and a preparing method of the traditional Chinese medicine preparation, belonging to the field of traditional Chinese medicines. The traditional Chinese medicine preparation is characterized by comprising the following components in weight percent: 40-55 parts of Tibet inula, 160-200 parts of rhubarb, 80-100 parts of Kaempferia galangal, 200-250 parts of Gypsum Gypsum Rubrum, 110-150 parts of myrobalan and 250-300 parts of Tronae. In the preparing method, on the basis of the traditional process, the original single crushing pattern is changed, a sterilization process is changed, i.e. the original moist heat sterilization process for medicine powder produced after medicinal slices are crushed is changed into a cobalt 60 irradiation process, and the pattern of collecting and coating volatile oil is changed. The preparing method sufficiently ensures that volatile components in the Tibet inula are not lost and oily components in the Tibet inula are not lost. In addition, the drug effect is more effectively ensured, the product name quality is ensured, and benefits of patients are guaranteed.

The invention relates to a botanical fungicide and the preparation method thereof, which relate to a fungicide and the preparation method. The invention solves the shortcomings of the existing fungicide in severe environmental pollution and resistance due to long-term use. The botanical fungicide of the invention is made from stellera chamaejasme, liquorice, aconite and radix sophorae falvescentis. The method of the invention comprises the steps of: 1. weighing and mixing the stellera chamaejasme, liquorice, aconite and radix sophorae falvescentis; 2. leaching the ethanol; 3. filtering; 4. distilling; 5. moist heat sterilization. The botanical fungicide of the invention has the advantages of innocuity, low residuals, small dosage, no pollution, no resistance, strong bacteriostatic activity, high selectivity, diversified raw material sources, easy match with other biological pesticides, etc. The method of the invention has the advantages of simple process, and low cost. Compared with the chemical agent, the Chinese herbal medicine fungicide has the advantages of many active sites, long agent duration, high degradation speed, small pollutions to the environments, etc.

The invention discloses a medical record cleaning and sterilizing device for hospital record management. The device comprises a device body, wherein a lower separation plate is arranged in the middle of the bottom surface of the inner cavity of the main body, an electric telescopic rod is mounted in a hollow groove of the inside of the lower separation plate, the top end of the electric telescopic rod is fixedly connected with the lower surface of a movable plate, the upper surface of the movable plate is in contact with an upper separation plate, the two sides of the upper separation plate are connected with a first cover plate and a second cover plate by hinges respectively, handles are mounted on the side surfaces of the first cover plate and the second cover plate, and a drying chamber is located below the first cover plate. According to the medical record cleaning and sterilizing device for hospital record management, a moist heat sterilization method is used, a pressure steam sterilization device kills microorganisms by denaturing protein and nucleic acid in microorganism thalli through high pressure saturated steam, the sterilization competence is high, the sterilization method is the most effective method in heat sterilization, and at the same time, papery medical records cannot be damaged by high temperature or disinfectant.

The invention discloses application of a cyclo olefin copolymer in preparing a biological and chemical container. When the cyclo olefin copolymer is applied to prepare the biological and chemical container, the prepared biological and chemical container has the following advantages: the glass bottle is resistant to heat and transparent, has high gas barrier property and low moisture absorption, can carry out high temperature moist heat sterilization and has good chemical resistance; and other high polymer materials such as plastic bottles are not easy to break, have light weight and can carry out high-energy ray radiation and sterilization with ethylene oxide.

The invention discloses a preparation method of a corn and soybean meal fermentation forage. The method comprises the following steps: mixing raw materials comprising corn and soybean meal with water according to a corn-to-soybean meal-to-water ratio of 1:0.8-1:1.2, uniformly stirring, and disinfecting at 100-115DEG C; inoculating microbes having an amount accounting for 20-30% of the weight of the raw materials, uniformly stirring, and fermenting at 35-45DEG C for 48-60h; and carrying out low temperature drying. The moist heat sterilization and the microbe treatment enable antigen proteins, raffinose having a flatulence effect and stachyose having the flatulence effect in the forage raw materials to be effectively degraded, and a large amount of beneficial metabolites and beneficial microbes to be accumulated. The method has the advantages of easy control of the fermentation process, and stable quality of products obtained in the invention.

The invention belongs to the technical field of chemical engineering and medicine, and discloses carbon microspheres loaded with metal nuclide as well as a preparation method and application. The method comprises the following steps: reacting metal ions with organic micromolecules in an aqueous solution to obtain a complex; adsorbing the complex by using carbon microspheres; and carrying out firsttreatment on the adsorbed carbon microspheres. The carbon microspheres loaded with the metal nuclide and prepared by the method can stably exist in the aqueous solution at the temperature lower than180 DEG C and at the pressure lower than 10 MPa, and the dissolution rate of the metal nuclide in the aqueous solution is lower than 0.1%; and the radionuclide release rate is still lower than 0.1% after heat and humidity sterilization at 121 DEG C for 15 minutes, and the safety risk of clinical use of radioactive microsphere products can be remarkably reduced.

The invention discloses a processing method of a dark tea aspergillus fermentation liquid tea drink. The method comprises the following steps: picking summer and autumn raw materials having one bud and five or six leaves and removing water and drying to prepare raw packed tea; crushing the raw packed tea and sieving with a 40-mesh screen and performing infusion, then carrying out suction filtration and sub-packaging, and performing moist heat sterilization at 121 DEG C for 25min for standby use; inoculating a fermentation agent in a fermentation tea soup culture medium and culturing in a constant-temperature oscillator; and filtering the fermented liquid, bottling and sterilizing. According to the method, tea leaves produced by relatively crude raw materials and unsalable and old tea leaves can be used for fermentation to increase the economic benefit, and no other assistant fermentation materials are used, so that the production cost can be reduced.

The invention provides a heat pump sterilization pool, mainly comprising a pool, a high-temperature heat pump system, a constant temperature system, a circulating filtration system, a water pump and a controller; the working temperature of the high-temperature heat pump system is over 20 DEG. C; the backwater of the pool is taken as heat source and is filtered through the circulating filtration system, and is heated to high-temperature hot-water or directly converted to vapor (at the temperature of 120 DEG. C) after entering into the high-temperature heat pump, keeping for 20 to 30 min; the high-temperature water is mixed after entering into the constant temperature system, and is adjusted to mild water, and is pumped into the pool by the water pump; the temperature and pressure parameter of the system are controlled by the controller; the effects of the invention are that: the heat source of the high-temperature heat pump utilizes the backwater heat of the pool; and the produced high-temperature water is converted into constant temperature water fitting doe the body temperature after adjusting by the constant temperature system; the backwater is carried out heating and moist heat sterilization when entering into the high-temperature heat pump through basic physical settlement filtering, which saves the cost of water treatment.

The present invention discloses one kind of composite culture medium and its preparation process and use. The halophilic bacteria culturing medium is prepared with waste water with salt content of 3-8 wt% from hot pickled mustard tuber production, and through regulating pH value with NaOH or HCl to 6.0-8.0, moist heat sterilization at 115-121 deg.c, adding agar in 1-2 wt% and moist heat sterilization at 115-121 deg.c. The halophilic bacteria culturing medium may be used in culturing halophilic microbe and is environment friendly.

The invention discloses a collagen dermal implant and a preparation method thereof. The method comprises the following steps of dissolving collagen in ultrapure water; performing refrigeration; adding a chemical cross-linking agent; performing uniform stirring at a low temperature; performing refrigeration; performing heating in a water bath to start a cross-linking reaction, so as to prepare cross-linked collagen gel ;adding normal saline-ammonia water into the cross-linked collagen gel, and performing a blocking reaction; adding normal saline into the cross-linked collagen gel after the blocking reaction for purification; granulating the purified cross-linked collagen gel by a screening method; and dispersing the granulated cross-linked collagen gel particles in normal saline to prepare a cross-linked collagen suspension; filling the cross-linked collagen suspension into a pre-filling injector; and performing moist heat sterilization to prepare the collagen dermal implant. The collagen obtained through fermentation has a physical filling function through the cross-linking reaction, and in addition, the collagen dermal implant is free of immunotoxic reaction, has good biocompatibility and can be used as a body tissue filler in medical cosmetology.

The invention discloses a tissue culture method of dendrobium stem seedlings. The method comprises serial steps of building an intermittent immersion solution culture system, wherein the steps comprise a first step of preparing and sterilizing an MS culture solution, wherein components and use amounts thereof of the MS culture solution are as follows: MS, 0.5 to 5.0 mg / L of NAA, 50 to 200 g / L of potato juice and 30 g / L of saccharose; adjusting a pH value to be 5.70 to 6.00; after the MS culture solution is prepared, placing the MS culture solution into a reagent bottle; transferring the reagent bottle into a moist heat high-pressure sterilization pot; sterilizing the reagent bottle by using a moist heat sterilization method for future use; a second step of sterilizing an intermittent immersion type bioreactor by using the moist heat sterilization method; a third step of inoculating explants into the intermittent immersion type bioreactor containing the MS culture solution; a fourth step of setting the relative parameters of the intermittent immersion type bioreactor. The method disclosed by the invention is used for breeding the dendrobium stem seedlings, and has the beneficial technical effects of high proliferation rate, high degree of automation, small amount of labor, good tissue culture seedling quality and high adaptability.

The invention provides a method for producing succinic acid by utilizing fermentation of actinobacillus succinogenes. The method includes taking canna edulis ker syrup as a main material, producing the succinic acid by using the fermentation of actinobacillus succinogenes CICC11014, determining the optical fermentation medium for bacterial strains through research for medium of bacterial strains; and fermenting the medium after optimization, wherein the medium comprises 89.66g / L of canna edulis ker syrup (by total suger), 38.55g / L of CSL, 2.23g / L of Na2HPO4*12(H2O), 2.23g / L of KH2PO4*3(H2O),60g / L of MgCO3, and 6.5 of the pH; and performing moist heat sterilization at a temperature of 115 DEG C for 20 minutes. The conversion rate of sugar can be improved through fermentation under the optical medium condition, so that the yield of the succinic acid can reach 70g / L.

The invention discloses a pharmaceutical composition containing difluprednate. The pharmaceutical composition is O / W emulsion and contains the active components of the difluprednate, one or more kinds of oil and one or more kinds of emulsion, auxiliary materials and water which serves as solvent, and the difluprednate is dissolved in the oil and / or the emulsion, wherein the pharmaceutical composition is prepared from, 0.01%-0.1% of the difluprednate, 1%-20% of the oil, 0.01%-10% of the emulsion and the balance water and auxiliary materials. In addition, the invention further provides a preparation method of the pharmaceutical composition containing the difluprednate. Compared with the prior art, according to the pharmaceutical composition containing the difluprednate and the preparation method thereof, the pharmaceutical composition is the emulsion, the average grain diameter of oil drops of the difluprednate emulsion is smaller than one micron, the pharmaceutical composition can be subjected to moist heat sterilization, grain size variation is not large after sterilization is conducted, and the grain size does not increase after long-term storage.

The invention discloses a simple sterilizing method for a long bacteria stick. The method comprises making of the bacteria stick, simple sterilization, inoculation and cultivation, and fruiting management. The simple sterilization of the long bacteria stick comprises sterilizing equipment and a sterilizing method, wherein the sterilizing equipment comprises a normal-pressure steam boiler and a sterilizing pile; the sterilizing pile comprises an air inlet pipeline, a sterilizing frame, an induction thermometer, an air exhaust pipe, a sealing layer, a heat-preserving stress layer and a blank pressing sand bag; the sterilizing method comprises bacteria stick loading, covering of the sealing layer and the heat-preserving stress layer, blank pressing, heating, heat preservation, fire ceasing, and cooling of a fruiting stick. By adopting the method, heat exchange between hot steam and the bacteria stick can be accelerated, the bacteria stick piling difficulty is lowered, deformation of the bacteria stick is effectively prevented, damp and hot sterilization can be ensured, the sterilizing effect is enhanced, and the formation of sterilization dead corners is prevented. Meanwhile, an accurate sterilization timing point can be introduced, the difficulty in determining the sterilizing time is lowered, and energy can be saved effectively.

A preparation method for an adsorbent capable of removing heavy metals such as Cu and Pb comprises: dropwise adding a 4M NaOH solution into a selective medium solution to adjust pH of the medium to 6-8; inoculating 2% (V / V) manganese oxidating bacteria pseudomonas sp.G7 strain to the medium solution which is subjected to heat sterilization with steam for 30 min at 115 DEG C, then adding a filtered disinfected buffer solution to again adjusting pH of the solution to 6-8; performing concussion culture for 12-24 h at 20-30 DEG C, adding filtered disinfected MnCl2 (to make the final concentration at 1 mM); continuously culturing for 1-3 weeks at 20-30 DEG C to aggregate a large amount of dark brown even black manganese oxides on the surface of bacterium colonies; and centrifuging with centrifugal force of 7500 g for 10-20 min to remove the medium, washing with a phosphatic buffer solution for 3-5 times, and finally dissolving surface adsorbed Mn<2+> with a 20-80 mM CuSO4 buffer solution to obtain biological manganese oxides.

The invention provides a preparation method for xylooligosaccharide. The preparation method comprises the following steps: smashing bagasse into powder, mixing the bagasse powder, water and cane molasses to obtain a first mixture; carrying out pH (Potential of Hydrogen) value regulation and temperature raising on the first mixture, adding a complex enzyme preparation, and carrying out constant temperature processing to obtain a second mixture; carrying out moist heat sterilization and cooling on the second mixture, adding an aspergillus niger strain, and carrying out stable culture and fermentation to obtain fermentation broth; carrying out plate-frame pressure filtration on the fermentation broth, and adding filtering and color removal auxiliary agent to obtain filter slags and clear solution; drying and smashing filter slags to obtain xylooligosaccharide used for feeding; carrying out vacuum spray drying on the clear solution to obtain high-purity xylooligosaccharide.