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Preparation process of composite medium and use thereof

A medium and liquid medium technology, applied in the field of microorganisms, can solve problems such as environmental governance difficulties, reduction of biodegradation power, and reduction of microbial respiration rate, and achieve the effects of reducing environmental protection pressure, reducing industrial production costs, and efficient utilization

Inactive Publication Date: 2007-07-18
ZHEJIANG UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

The advantage is that the medium components are fixed and the microbial culture is stable; the disadvantage is that the cost is high and the method is cumbersome
[0003] Due to the high concentration of inorganic salts in pickled mustard sewage, common biological treatment methods are difficult to work. The main performance is that the removal rate of organic matter in the sewage treatment system is reduced, the respiration rate of microorganisms is reduced, and the high concentration of salt interferes with the normal metabolic function of cells and reduces the biodegradation power The reduction of biological species in the treatment system affects the normal flocculation and sedimentation process of sludge, etc., which brings great difficulties to the environmental management of mustard mustard production areas.

Method used

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  • Preparation process of composite medium and use thereof

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Effect test

Embodiment 1

[0018] Embodiment 1: Liquid mustard mustard wastewater culture medium configuration method

[0019] The mustard mustard wastewater was directly taken from the liquid culture medium, adjusted to pH 7.0 with NaOH or HCl, and sterilized by moist heat at 121°C for 30 minutes.

Embodiment 2

[0020] Embodiment 2: Solid mustard mustard wastewater culture medium configuration method

[0021] Add 15g / L agar to the liquid medium, and sterilize with damp heat at 121°C for 30 minutes.

Embodiment 3

[0022] Embodiment 3: component analysis of mustard mustard wastewater

[0023] Wastewater from mustard processing plants in Ningbo, Zhejiang was collected, and the composition of basic elements was analyzed by ion chromatography (Table 1). This work was mainly completed by the Analysis and Testing Center of Zhejiang University. The main cation of pickled mustard wastewater is Na + , the anion is Cl - , the NaCl content is 3% to 5%, and the salinity is 3% to 8%. According to the variety and quantity analysis of the main raw and auxiliary materials for mustard production, the sewage is rich in plant fiber, plant protein, organic acids, alcohols, sodium chloride, calcium, magnesium and other inorganic elements. The rich nutrients make the mustard sewage a microorganism Especially good medium for halophilic microorganisms.

[0024] Anion and cation content (ion chromatography, g / L) in the sewage stock solution of table 1:

[0025] ion type

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Abstract

The present invention discloses one kind of composite culture medium and its preparation process and use. The halophilic bacteria culturing medium is prepared with waste water with salt content of 3-8 wt% from hot pickled mustard tuber production, and through regulating pH value with NaOH or HCl to 6.0-8.0, moist heat sterilization at 115-121 deg.c, adding agar in 1-2 wt% and moist heat sterilization at 115-121 deg.c. The halophilic bacteria culturing medium may be used in culturing halophilic microbe and is environment friendly.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a preparation method and application of a compound culture medium. Background technique [0002] The medium for the isolation and cultivation of halophilic microorganisms is generally obtained by moist heat sterilization of precisely quantitative salt ions, peptone and yeast extract as raw materials. Its advantage is that the composition of the medium is fixed, and the microbial culture is stable; the disadvantage is that the cost is high and the method is cumbersome. [0003] Due to the high concentration of inorganic salts in pickled mustard sewage, common biological treatment methods are difficult to work. The main performance is that the removal rate of organic matter in the sewage treatment system is reduced, the respiration rate of microorganisms is reduced, and the high concentration of salt interferes with the normal metabolic function of cells and reduces the biod...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20
Inventor 吴敏许学伟孟凡旭
Owner ZHEJIANG UNIV
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