41 results about "Halophilic microorganisms" patented technology

the invention discloses an extracting method of total plant protein and specific extracting liquid, which comprises the following parts with pH value at 7.5-8.5: 80-120mM EDTA, 80-120mM trimethyl aminomethane, 40-60mM sodium tetraborate and 40-60mM vitamin C, 0.8-1.2% polyvinyl polypyrrolidon,0.8-1.2% Triton-100, 1.5-2.5% 2-mercaptoethanol and 28-32% saccharose solution.

The invention provides a device for treating waste water containing salt through coupling of electrodes and anaerobic organisms, which comprises a cylindrical anaerobic reactor, the lower part of the anaerobic reactor is provided with a water distributor, the middle is provided with an anode and a cathode and connected with an external power supply through a wire, the upper part is provided with a three-phase separator, and the external is provided with a thermal insulating layer. The anode and the cathode are coaxially inserted into the 1 / 4 part of the effective height of the reactor, the lengths of the anode and the cathode are 50% of the effective height of the whole reactor, and externally applied voltage is optimally 1.2V. In the technology, iron-carbon electrodes are arranged in the anaerobic reactor for treating waste water containing salt with a high electricity conducting rate, the activity of anaerobic microorganisms is enhanced under the coupling action of an electric field and ferrous ion release, and the dwelling time of sludge in the reactor is prolonged, so that the metabolism speed of the microorganisms is raised, and the biomass is increased. The device is favorable to sieving efficient halophilic microorganisms, is used for treating high concentration of organic waste water containing salt, and has remarkable innovation, good effect for treating the waste water and good application prospect.

The object of the present invention is to provide a method for producing ectoine and hydroxy ecto by cultivating halophilic microorganisms with reasonable cost to improve the synthesis efficiency of ectoine and hydroxy ecto. The method for cultivating halophilic microorganisms of the present invention to produce hydroxyectoine comprises the following steps: (1) strain activation; (2) seed cultivation; (3) fermentation stage; (4) reducing the salt concentration of the culture medium by feeding Rapidly increase to 3-3.5M, and then continue to cultivate for 3-8h, so that the synthesis of ectoine is greatly increased; (5) raise the culture temperature from the optimal growth temperature to a higher temperature, continue to cultivate for 3-10h, and obtain a higher High yield of ectoine synthesis. In the invention, the fermentation inoculation strain is cultivated by means of osmotic pressure shock and heat shock in the fermentation process, and the synthesis rate of ectoine and hydroxy ectoine is greatly improved at a reasonable cost.

The invention provides a method for treating high-salt wastewater by using halophilic microorganisms. The halophilic microorganisms can adapt to a high-salt environment to perform normal growth metabolism, and comprise haloduric and halophilic bacteria, yeasts, archaea and microfauna. The method for treating the high-salt wastewater by using the halophilic microorganisms comprises the following steps: adding a halophilic microbial flora into a biochemical system; performing mixed culture on the halophilic microbial flora in an influent salt concentration range of 1-25% to improve the pollution degradation capability of the halophilic microbial flora and obtain a halophilic microbial aggregate with good mud-water separation performance and precipitation performance, such as active sludge and a biomembrane; and stabilizing the process parameters and influent water quality of the biochemical system to ensure that the biochemical effluent reaches a standard or meets design requirements. By adopting the method provided by the invention, the high-salt wastewater with the influent salt concentration of 1-25% of the biochemical system can be subjected to biochemical treatment, and the halophilic microbial agent only needs to be added once, so that the investment and operation costs can be reduced.

The invention discloses a method for culturing halophilic granular sludge, and belongs to the field of biological treatment of sewage. The principles of the invention are as follows: (1) water inflowload is rapidly increased, and EPS secretion of halophilic microorganisms is stimulated. The microorganisms grow in an attached manner through the binding action of EPS, and mature halophilic active granular sludge is formed under the action of hydraulic shearing force; and (2) long precipitation time and short precipitation time act in a combined manner. At the long precipitation time in the early stage, growth of the halophilic microorganisms in a reactor is achieved; and at the short precipitation time in later stage, selective pressure is provided for granulation. According to the halophilic active granular sludge formed by culture in the invention, sludge sources are various, the technology is simple, the salinity tolerance range is between 1% and 10% due to the fact that the enrichedmicroorganisms come from the halophilic flora of settled sludge at a sea inlet, and a compact morphological structure can be kept. The precipitation time is greatly shortened, the treatment period iseffectively shortened, the treatment effect is guaranteed, rapid treatment of the high-salt wastewater is realized, the short-cut nitrification is realized, and the energy consumption is reduced by 30%.

The invention discloses a compound halophilous-microorganism bacterium agent and an application thereof. Halophilous microorganisms are the microorganisms which are not inhibited or poisoned by high-concentration inorganic salts in the high-salt environment, can carry out normal growth and metabolism and comprise salt-resistant microorganisms and halophilic microorganisms. The compound halophilous-microorganism bacterium agent at least comprises two of halophilic bacteria, halophilic archaea, salt-resistant bacteria, salt-resistant archaea, halophilic fungi and salt-resistant fungi, wherein the types and the quantity of the halophilous microorganisms are at least 10, and the halophilous microorganisms at least comprise one type of uncultured microorganisms. The compound halophilous-microorganism bacterium agent disclosed by the invention has the advantages that high-efficiency and stable degradation can be carried out on organic matters under the condition of 1%-20% salt; by using the compound halophilous-microorganism bacterium agent for biochemical treatment of high-salt waste water and microorganism restoration of saline-alkaline soil, the pollution treatment cost can be reduced.

A alternately false single afterbirth bacterium HH407( Pseudoalteromonas flavipulchra HH407) CCTCC N0: M207009. This bacterium can grow up under 0degree C, the growth temperature of this bacterium scope is 0-35 degree C, the most suitable growth temperature is 20degree C; Growth pH scope is 5.5-11, most suitable growth pH is 8.5; The NaCl density of growth is 0.5%-13%, the optimal growth of NaCl concentration of 4%, no growth without NaCl, it is a typically moderate marine Psychrophilic Halophilic microorganisms; bacteria can use pancreatic peptone, yeast extract, peptone, cellobiose, starch, gelatin, acetic acid, citric acid, methanol and ethanol. The present invention also opens an alternate use of low-temperature alkaline-producing Pseudomonas methods and by the method of low-temperature alkaline protease products. The low-temperature alkaline protease can be used as the main enzyme detergent additives can be directly room temperature washing, saves energy; and in food manufacturing, leather, silk, environmental protection, medicine and other fields also has broad application prospects.

The invention relates to a treatment method of phenolic wastewater in production of sebacic acid. The treatment method comprises the steps that high salt phenolic wastewater is subjected to extracting and phenol removing by an extraction agent, and then biochemical treatment is performed by using halophilic microorganisms, so that CODCr and volatile phenol in the wastewater can reach the standard, meanwhile phenolic substances in the wastewater are recycled; the extraction agent is selected from at least one of phosphate esters, alkylamines, castor oil, kerosene or diesel oil or a mixture thereof. The treatment method adopts a method of combination of physical and chemical extraction and halophilic microorganism biochemical treatment, by firstly utilizing an efficient extraction agent, the phenolic substances in the wastewater are removed and recycled, meanwhile the CODCr and the phenol concentration of the wastewater are reduced; then by utilizing halophilic microorganism flora, the extracted wastewater is not subjected to a desalination technology or attenuation, but the biochemical treatment is performed directly, so that the CODCr and the phenol content in the wastewater can reach the standard, and the wastewater treatment cost is reduced.

The invention provides a method for carrying out raw soil greening on slight saline and alkaline land by using a halophilic microorganism -containing water retaining agent. The water retaining agent comprises starch, acrylic acid, acrylamide, halophilic microorganisms, soybean stalk powder, rice straw powder, elm leaf powder and walnut leaf powder. A preparation method of the water retaining agent comprises starch treatment, and treatment of every leaf powder comprises the steps of starch pasting, starch solution preparation, copolymerization reaction and product precipitation. The invention also provides an application of the water retaining agent in plantation of cherry trees in saline and alkaline land. The water retaining agent prepared in the invention has strong water absorption ability in tap water, rainwater and a 0.6% NaCl solution, the water absorption ability in the tap water can reach 950-1150 times the self weight, the water absorption ability in the rainwater can reach 820-960 times the self weight, and the water absorption ability in the 0.6% NaCl solution can reach 470-600 times the self weight.

The invention relates to a device for processing high-salinity degradation-resistant waste water. The device comprises a salinity homogenizing pool and an anaerobic device, wherein the anaerobic device is internally provided with a plurality of suspended filler layers and a plurality of sections of baffle plates from bottom to top, and halophilic microorganisms are adhered on the suspended filler layers. The invention further relates to a method for processing high-salinity degradation-resistant waste water. The method comprises the steps of domesticating, pretreating and bioprocessing halophilic microbial communities. According to the device and the method, the scope of salinity of the inflowing water is controlled through the salinity homogenizing pool, the anaerobic device is internally provided with the plurality of suspended filler layers and the plurality of sections of baffle plates, different grades of halophilic microorganism communities are adhered on the suspended filler layers, so that the anaerobic device and the treating method can adapt to waste water with gradient-changed salinity, reduce the influence of salinity change on the waste water treatment effect, and also improve the sludge concentration in the anaerobic device, and therefore, the degradation rate of organisms is improved. The device and the method are strong in salinity adaptability and wide in application range.

The present invention discloses one kind of composite culture medium and its preparation process and use. The halophilic bacteria culturing medium is prepared with waste water with salt content of 3-8 wt% from hot pickled mustard tuber production, and through regulating pH value with NaOH or HCl to 6.0-8.0, moist heat sterilization at 115-121 deg.c, adding agar in 1-2 wt% and moist heat sterilization at 115-121 deg.c. The halophilic bacteria culturing medium may be used in culturing halophilic microbe and is environment friendly.

The invention discloses a method for separating and extracting tetrahydropyrimidine from halophilic microbial fermentation liquid. The method comprises the following operating steps: carrying out primary centrifugation, soaking and swelling, carrying out secondary centrifugation, concentrating, removing protein bacteria, carrying out salt removal, concentrating and crystallizing to obtain tetrahydropyrimidine crystals. The method has the beneficial effects that (1) tetrahydropyrimidine is extracted from the halophilic microbial fermentation liquid by using an ethanol swelling method; the ethanol molecules are small and are fast in diffusion speed, so that the method greatly increases the speed compared with a process dissolving thalluses by adding pure water; (2) a tetrahydropyrimidine substance in cells is released to the utmost extent by means of a reasonable ratio of solid to liquid, so that the tetrahydropyrimidine substance with a high concentration can be obtained; (3) the centrifugation process is performed for twice so as to remove sodium chloride, the thalluses and protein in the fermentation liquid, so that the processing burden of subsequent processes is greatly reduced;(4) after extraction is finished, the ethanol can be recycled, so that the resources are saved, and the aim of recycling is achieved.

The invention provides a method for treating spent caustic wastewater by utilizing halophilic microorganism. The method comprises the steps that the spent caustic wastewater is adsorbed by polyurethane sponge adsorption particles, then subjected to treatment through a halophilic microorganism reactor, and spent caustic wastewater treatment can be achieved after the spent caustic wastewater is treated through a hydrolysis acidification pool, a contact oxidation tank, a primary settling pond, an ozonator, a biological aerated filter, a second settling pond, an ultrafiltration membrane and a reverse osmosis membrane, meanwhile, recycling of oil is achieved, the energy is saved, the cost is lowered, and the purposes of economic benefits and environmental benefits are achieved at the same time.

The invention discloses a method for processing preserved kumquat processing waste water rich in salts and organic matters. The method mainly comprises following six steps: (1) adding quicklime into waste water, and stirring to fully carry out reactions; (2) filtering the solution by a micro filtration machine; (3) adding quicklime, PAC, and PAM, and carrying out coagulation air floatation; (4) adding H2SO4 to adjust the pH to 8.5; (5) inoculating halophilic microorganisms to carry out anaerobic baffle board reactions (ABR); and (6) carrying out halophilic primary contact oxidation and halophilic secondary contact oxidation. By adding medicaments, under the cooperative effect of micro filtration machine and coagulation air floatation, suspensions such as fruit dregs, coked calcium sulfite, and the like, and part of macromolecular organic matters are effectively removed, and an ideal pretreatment effect is guaranteed. The problems that common active sludge cannot exist in waste water rich in salts and organic matters and the biochemical treatment effect is bad are solved.

The invention discloses a saline-alkaline tolerance rapeseed pelleting formula which is prepared from an adhesive, an inertia filling material, an additive and a coloring agent, wherein the inertia filling material is prepared from talcum powder and bentonite; the inertia filling material is prepared from talcum powder and bentonite; the additive is prepared from biological colonies and a solution (SY solution) containing super-halophilic microorganisms and 98 percent of polyglutamic acid. The invention further discloses a processing method of pelleting coats of saline-alkaline tolerance rapeseeds. According to the saline-alkaline tolerance rapeseed pelleting formula and the processing method, absorption of crops for nitrogen, phosphorus and potassium can be enhanced, the content of nitrate in a plant is reduced, growth of a plant root is promoted, and the resistance to root rot and pathogenic bacteria such as grey mould is enhanced; meanwhile, the cold resistance and flood resistance of the crops are improved. Halophilic bacteria can remove phosphate from salt-containing water or alkaline water to form struvite, and the phosphate is removed by a biological method.

The invention discloses a desalination method for cooperatively treating edible fungus salinizing water based on halophilic microorganism complex inoculants and a three-stage type membrane technology. The method comprises the following steps: adding a microorganism complex inoculant I into the edible fungus salinizing water, and aerating and culturing for 5 days; then, carrying out three-stage type membrane treatment on the salinizing water to realize desalting; and finally, adding a microorganism complex inoculant II into the salinizing water subjected to three-stage type membrane treatment, and aerating and culturing for 4 days. The method disclosed by the invention has the advantages that the cost is reduced, the treatment method is more ecological and safer; a desalination rate of the finally obtained edible fungus salinizing water can be over 97%.

The invention provides an improved method for cultivating halophilic microorganisms. According to the method, NaCl which is conventionally used for providing a high-salt environment for the halophilicmicroorganisms is completely or partially replaced by other sodium salts, and therefore the chloride ion concentration in the fermentation system can be reduced, so that corrosion of chloride ions inthe fermentation system to a fermentation device is reduced, subsequent wastewater treatment is facilitated, and microbial contamination during open fermentation is reduced; and the invention also relates to a culture medium for the cultivation.

The invention discloses a method for enhancing salt resistance of turf grass by using halophiles, relating to the technical field of biological restoration of salinized soil. The method comprises the following steps: (1) culture of a halophile bacterial liquid; (2) preparation of a bacterial suspension; (3) preparation of a composite halophile bacterial fertilizer; and (4) turf planting. The fertility condition of soil is improved; the plants adsorb salts; the turf glass can form a compact withered grass layer, thereby increasing the overland runoff of rainfall and reducing the non-point pollution; the microbe culture medium wood dust ensures the supply of microbial growth nutrients, enhances the operability of implanting microbes into soil, is capable of improving the soil environment, and effectively utilizes the wastes; and the method is low in cost and simple to operate.

The invention provides a biological autotrophic nitrogen removal integrated constructed wetland system which comprises a sulfur autotrophic denitrification wetland subsystem which comprises a first filler and sulfur autotrophic denitrification bacteria; and an autotrophic nitrification wetland subsystem which comprises a second filler and autotrophic nitrifying bacteria, wherein the sulfur autotrophic denitrification wetland subsystem is communicated with the autotrophic nitrification wetland subsystem, and sewage flows in from the bottom of the sulfur autotrophic denitrification wetland subsystem, circulates between the sulfur autotrophic denitrification wetland subsystem and the autotrophic nitrification wetland subsystem, and is discharged until the water quality of the sewage meets a preset standard. Microorganisms on which the system depends are salt-tolerant (or halophilic) microorganisms existing in the mangrove forest wetland system, nitrogen pollutants and organic pollutants in the mariculture sewage can be effectively removed after operation and debugging, and the decontamination efficiency of the wetland is improved.

The invention provides a portable high pressure microorganism transferring and culturing device. The portable high pressure microorganism transferring and culturing device comprises a pressurization interface unit, a high pressure airtight unit and a high pressure culture unit, wherein the pressurization interface unit is connected with a high pressure source and controlled to open or close by virtue of a handle-free type needle valve; the high pressure airtight unit is connected with the pressurization interface unit and the high pressure culture unit and provides a long-term high pressure airtight environment for a culture system; the high pressure culture unit provides a non-pollution high pressure culture space for microorganisms. The portable high pressure microorganism transferring and culturing device provided by the invention has the advantages that all the components are vertically arranged, no protruding structure is formed, and the handle-free high pressure needle valve is adopted, so that pressure relief caused by looseness of the valve and the interface owing to jolt during long-distance transportation is avoided; all the components can be disassembled, cleaned and disinfected, so that the non-pollution culture environment is guaranteed; a main structure is simple, weight is light, and carrying and long-distance transportation are convenient; manufacturing cost is low, and the portable high pressure microorganism transferring and culturing device can be conveniently connected with multiple pressurization equipment and can be widely applied to culture of multiple barophilic microorganisms including halophilic microorganisms, thermophilic microorganisms and psychrophilic microorganisms.

The invention relates to a marine active sludge product, and a preparation method and a water treatment application of the marine active sludge product. The marine active sludge product is rich in salt-tolerant microorganisms and halophilic microorganisms from the ocean or other salt environments, and exists in a form of particle flocculent active sludge or a separation composite inoculant; the product has a DO (dissolved oxygen) content of 0.1-4.0mg / L, an ambient temperature of 0-50 DEG C, a salt concentration of 2-34% and a pH (potential of hydrogen) value of 5-9; and when the product exists in the form of the active sludge, an SVI (sludge volume index) of the product is 50-200mL / g, and an MLVSS (mixed liquor volatile suspended solid) is 1.2-7.5g / L. According to the preparation method, a halophilic microorganism culture technology and an active sludge culture technology are fused for use innovatively; a novel microorganism purification system for high-salt organic wastewater treatment is prepared; an operating system with high wastewater degradation capability, and economical operating cost and temperature is achieved; and an application range is wide. The invention further provides a relevant water treatment method. The water treatment capability and the application range of the marine active sludge product is improved and expanded, and the practicability is better.

The invention relates to the technical field of microbial compound fertilizers, in particular to a preparation method of microbial granules, which comprises the following steps: step 1, adding beneficial microbial liquid into a mixed nutrient solution, adding porous hydroxyapatite into the mixed nutrient solution, and carrying out aeration treatment to obtain microbial-loaded porous hydroxyapatite; step 2, uniformly mixing the microorganism-loaded porous hydroxyapatite and an ultrafine protein powder, and adding the mixture into a granulator for granulation to obtain beneficial bacterium granules; and step 3, adding the beneficial bacterium granules and patite loaded with a halophilic microorganisms into a pelletizer for pelletizing to obtain a product. The problem that in the prior art, microbial strains make direct contact with compound fertilizer, and consequently the survival rate of microorganisms is low is solved. The beneficial bacteria grains in the microbial grains are used asinner cores, and the apatite loaded with halophilic microorganisms is used as an isolation layer. Due to the existence of the apatite loaded with halophilic microorganisms, beneficial bacteria grainscan be protected, so that the survival rate of the beneficial bacteria is improved.

The invention provides a method for performing original soil greening on saline-alkali land with an under-mulch drip irrigation technology using an improver containing cow dung fermentation liquor. The method includes the steps of saline-alkali land improvement, irrigation and greening planting. The step of irrigation includes the mixing process, wherein in the mixing process, the improver and clean water are mixed according to a certain volume ratio. The improver is prepared from halophilic microorganisms, bacillus mucilaginosus, alanine, xylanase, quail dung fermentation liquor, compound sodium nitrophenolate, chlorogenic acid, mannose, propiconazole, camphor oil, astaxanthin, hydroxycoumarin, ammonium bicarbonate, triple superphosphate, potassium carbonate, magnesium nitrate, ferrous sulfate, manganese chloride, dehydrated borax and cupric acetate. By means of the original soil greening method, plant growth is promoted; with Iris lacteal as an example, the green period lasts for 300-320 days, and the flowering phase lasts for 60-80 days; the medicinal value is high, and the irisquinone content is high; water resources are greatly saved, and the water resource utilization rate is increased.

The invention discloses an organic microbial compound fertilizer for improving saline-alkali soil and a preparation method of the fertilizer, and belongs to the technical field of fertilizer preparation, the organic microbial compound fertilizer for improving saline-alkali soil comprises the following components of high saline-alkali phagocytic bacteria, medium-low saline-alkali phagocytic bacteria and a core bacterial fertilizer, and obligate basophilic bacteria and extreme halophilic microorganisms are mixed; facultative basophilic bacteria, moderate halophilic microorganisms and weak halophilic microorganisms are mixed; bacillus mucilaginosus, a three-element fertilizer and bacillus thuringiensis are mixed and granulated; the medium-low saline-alkali phagocytic bacteria is placed in a roller, the core bacterial fertilizer is placed in the roller the, stirring and mixing are implemented to make the medium-low saline-alkali phagocytic bacteria adhere to the surface of the core bacterial fertilizer, the high saline-alkali phagocytic bacteria is placed in the roller, and continuously stirring and mixing is implemented to make the high saline-alkali phagocytic bacteria adhere to theouter wall of the medium-low saline-alkali phagocytic bacteria. The saline-alkali soil is improved in a layer-by-layer decomposition mode, growth of crops can be effectively guaranteed, and the yieldof the crops is increased.

The invention relates to a device for processing high-salinity degradation-resistant waste water. The device comprises a salinity homogenizing pool and an anaerobic device, wherein the anaerobic device is internally provided with a plurality of suspended filler layers and a plurality of sections of baffle plates from bottom to top, and halophilic microorganisms are adhered on the suspended filler layers. The invention further relates to a method for processing high-salinity degradation-resistant waste water. The method comprises the steps of domesticating, pretreating and bioprocessing halophilic microbial communities. According to the device and the method, the scope of salinity of the inflowing water is controlled through the salinity homogenizing pool, the anaerobic device is internally provided with the plurality of suspended filler layers and the plurality of sections of baffle plates, different grades of halophilic microorganism communities are adhered on the suspended filler layers, so that the anaerobic device and the treating method can adapt to waste water with gradient-changed salinity, reduce the influence of salinity change on the waste water treatment effect, and also improve the sludge concentration in the anaerobic device, and therefore, the degradation rate of organisms is improved. The device and the method are strong in salinity adaptability and wide in application range.

The invention discloses a synergistic treatment method of cold and drought area mine wastewater and domestic sewage. The method includes the steps: pretreating mine wastewater, and removing solid objects in the mine wastewater in a precipitation manner; performing biochemical treatment, leading pretreated mine wastewater into distributing wells below frozen soil layers, leading pretreated mine domestic sewage into the distributing wells, performing biochemical treatment, filling the bottom of the distributing wells with activated sludge with halophilic microorganisms; blowing hot air into thedistributing wells by an aerating device when the temperature in the distributing wells is lower than survival temperature of the halophilic microorganisms, and maintaining the temperature in the distributing wells to be lower than 12 DEG C. According to the method, mining wastewater can be treated in successive years in cold and drought areas in a lower cost manner, external temperature effect isavoided, nutrient elements cannot be additionally added in the biochemical treatment process, and ground plants can be nursed by microorganism metabolites.

The invention relates to a semi-continuous culture method capable of treating salt-containing wastewater. According to the semi-continuous culture method, inorganic nutrients such as nitrogen salt and / or phosphorus salt in the salt-containing wastewater are recycled by culturing halophilic microorganisms in the salt-containing wastewater. According to the semi-continuous culture method, the salt-containing wastewater can be treated on a large scale, so that the salt-containing wastewater meets the discharge standard, the treated salt-containing wastewater can be used for producing table salt, and the semi-continuous culture method has great cost benefits and economic benefits.

the invention discloses an extracting method of total plant protein and specific extracting liquid, which comprises the following parts with pH value at 7.5-8.5: 80-120mM EDTA, 80-120mM trimethyl aminomethane, 40-60mM sodium tetraborate and 40-60mM vitamin C, 0.8-1.2% polyvinyl polypyrrolidon,0.8-1.2% Triton-100, 1.5-2.5% 2-mercaptoethanol and 28-32% saccharose solution.

The present invention relates to an aqueous composition comprising cells of at least one strain of a halophilic microorganism, and alkali sulfate in a concentration of at least 30 g / l based on the total volume of the aqueous composition. The present invention further relates to a method for treating a waste water, comprising obtaining or providing a waste water, contacting said waste water with cells of at least one strain of a halophilic microorganism, and thereby generating an aqueous composition comprising alkali sulfate in a concentration of at least 30 g / l, and incubating said aqueous composition under conditions which allow for the treatment of the waste water.