Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for separating and extracting tetrahydropyrimidine from halophilic microbial fermentation liquid

A technology for halophilic microorganisms and tetrahydropyrimidines, applied in the field of separation and extraction of tetrahydropyrimidines, can solve the problems of wasting water resources, unfavorable environment, shortening service life and the like, and achieves the effects of reducing processing burden, fast diffusion and improving speed.

Inactive Publication Date: 2018-12-21
FUTASTE PHARM CO LTD
View PDF6 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The first method of this method is to use the mechanical method of crushing the dried bacteria, which consumes a lot of energy and is not conducive to the full extraction of intracellular products. The second is to add water to dissolve it. Unfavorable environment
The patent document titled "A Method for Separating and Extracting Ectoine from Fermentation Broth" discloses a method for extracting ectoine using a double-membrane system. Although this method has low energy consumption, the bacteria and Protein can easily block the membrane pore size, which greatly shortens the service life of the membrane, which is not in line with the economics of actual production
In the patent titled "Method for Cultivating Halophilic Microorganisms to Produce Ectoline and Hydroxyectoine", it only mentions the release of intracellular products by ethanol extraction, but for the specific method of ethanol extraction and how to extract ectoine This intracellular product is released to the maximum extent, but there is no detailed description and discussion

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) One-time centrifugation: Take 6L of halophilic microbial fermentation broth rich in ectoine, centrifuge in a high-power centrifuge to obtain 141 grams of bacteria, and the centrifuge speed is 4500 rpm;

[0028] 2) Soaking and swelling: Add 1200ml of ethanol solution with a mass percentage concentration of 60% to the bacteria, soak for 4 hours, so that the ethanol molecules penetrate into the inside of the cell, the cell volume expands, the cell wall ruptures, and the ectoine inside it is released into the solution;

[0029] 3) Secondary centrifugation: centrifuge the solution again in a high-power centrifuge at a centrifugal speed of 4600 rpm, discard the centrifuged cells, and obtain 1185ml of supernatant;

[0030] 4) Concentration: Evaporate the supernatant, recover the ethanol in the supernatant, add 1000ml of pure water to the evaporated and concentrated supernatant to dilute;

[0031] 5) Protein removal: pass the supernatant through a ceramic membrane with a por...

Embodiment 2

[0035] 1) One-time centrifugation: take 6L of halophilic microbial fermentation broth rich in ectoine, centrifuge in a high-power centrifuge to obtain 123 grams of bacteria, and the centrifuge speed is 5000 rpm;

[0036] 2) Soaking and swelling: add 1500ml of ethanol solution with a mass percentage concentration of 70% to the bacteria, and soak for 3 hours, so that the ethanol molecules penetrate into the cell, the cell volume expands, the cell wall ruptures, and the ectoine inside it is released into the solution;

[0037] 3) Secondary centrifugation: centrifuge the solution again in a high-power centrifuge at a centrifugal speed of 4700 rpm, discard the centrifuged bacteria, and obtain 1455ml of supernatant;

[0038] 4) Concentration: Evaporate the supernatant, recover the ethanol in the supernatant, add 1350ml of pure water to the evaporated and concentrated supernatant to dilute;

[0039] 5) Protein removal: pass the supernatant through a ceramic membrane with a pore size ...

Embodiment 3

[0043] 1) One-time centrifugation: Take 6L of halophilic microbial fermentation broth rich in ectoine, centrifuge in a high-power centrifuge to obtain 98 grams of bacteria, and the centrifuge speed is 4500 rpm;

[0044] 2) Soaking and swelling: Add 1000ml of ethanol solution with a mass percentage concentration of 50% to the bacteria, and soak for 8 hours, so that the ethanol molecules penetrate into the cell, the cell volume expands, the cell wall ruptures, and the ectoine inside it is released into the solution;

[0045] 3) Secondary centrifugation: Centrifuge the solution again in a high-power centrifuge at a centrifugal speed of 4600 rpm, discard the centrifuged cells, and obtain 985ml of supernatant;

[0046] 4) Concentration: Evaporate the supernatant, recover the ethanol in the supernatant, add 1100ml of pure water to the evaporated and concentrated supernatant to dilute;

[0047] 5) Protein removal: pass the supernatant through a ceramic membrane with a pore size of 0....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Apertureaaaaaaaaaa
Conductivityaaaaaaaaaa
Conductivityaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for separating and extracting tetrahydropyrimidine from halophilic microbial fermentation liquid. The method comprises the following operating steps: carrying out primary centrifugation, soaking and swelling, carrying out secondary centrifugation, concentrating, removing protein bacteria, carrying out salt removal, concentrating and crystallizing to obtain tetrahydropyrimidine crystals. The method has the beneficial effects that (1) tetrahydropyrimidine is extracted from the halophilic microbial fermentation liquid by using an ethanol swelling method; the ethanol molecules are small and are fast in diffusion speed, so that the method greatly increases the speed compared with a process dissolving thalluses by adding pure water; (2) a tetrahydropyrimidine substance in cells is released to the utmost extent by means of a reasonable ratio of solid to liquid, so that the tetrahydropyrimidine substance with a high concentration can be obtained; (3) the centrifugation process is performed for twice so as to remove sodium chloride, the thalluses and protein in the fermentation liquid, so that the processing burden of subsequent processes is greatly reduced;(4) after extraction is finished, the ethanol can be recycled, so that the resources are saved, and the aim of recycling is achieved.

Description

technical field [0001] The invention relates to the field of biotechnology for extracting ectoine, in particular to a method for separating and extracting ectoine from fermentation broth of halophilic microorganisms. Background technique [0002] Halophilic microorganisms are bacilli that can ferment to produce ectoine. Ectopyrimidine is a substance produced by this halophilic microorganism inside the cell, which can protect cells, proteins, cell membranes and cells in extreme environments. It has the functions of protecting the immune system, synthesizing heat shock proteins, and protecting membrane structures. In addition, ectoine has moisturizing, anti-ultraviolet and anti-aging functions on human skin, and can be used in biomedicine, fine chemicals and other fields. [0003] To obtain ectoine, the ectoine in the bacterial cells must be released into the solution. In the patent literature for the separation and extraction of ectoine, the patent name is "A kind of extrac...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07D239/06
CPCC07D239/06
Inventor 邱学良孙鲁周娟黄伟红崔静曹玉华董丽红堵国成
Owner FUTASTE PHARM CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com