Portable high pressure microorganism transferring and culturing device

Abstract

The invention provides a portable high pressure microorganism transferring and culturing device. The portable high pressure microorganism transferring and culturing device comprises a pressurization interface unit, a high pressure airtight unit and a high pressure culture unit, wherein the pressurization interface unit is connected with a high pressure source and controlled to open or close by virtue of a handle-free type needle valve; the high pressure airtight unit is connected with the pressurization interface unit and the high pressure culture unit and provides a long-term high pressure airtight environment for a culture system; the high pressure culture unit provides a non-pollution high pressure culture space for microorganisms. The portable high pressure microorganism transferring and culturing device provided by the invention has the advantages that all the components are vertically arranged, no protruding structure is formed, and the handle-free high pressure needle valve is adopted, so that pressure relief caused by looseness of the valve and the interface owing to jolt during long-distance transportation is avoided; all the components can be disassembled, cleaned and disinfected, so that the non-pollution culture environment is guaranteed; a main structure is simple, weight is light, and carrying and long-distance transportation are convenient; manufacturing cost is low, and the portable high pressure microorganism transferring and culturing device can be conveniently connected with multiple pressurization equipment and can be widely applied to culture of multiple barophilic microorganisms including halophilic microorganisms, thermophilic microorganisms and psychrophilic microorganisms.

Description

technical field [0001] The invention relates to an experimental device in the technical field of microbiological cultivation experiments, in particular to a portable high-pressure microorganism transfer and cultivation device. Background technique [0002] The microbial biomass in the abyssal biosphere accounts for 90% of the global total. The microorganisms detected from the deep sea are significantly different from those in other environments, and have unique metabolic pathways, signal transduction and defense mechanisms. They are living fossils for the study of early life processes and the evolution of the earth's environment, and have irreplaceable scientific value. High pressure is a basic environmental parameter that distinguishes the deep sea from other ecosystems. 70% of the ocean is in a high-pressure environment above 38MPa. Therefore, deep-sea microorganisms have adapted to the high-pressure environment and are pressure-resistant and even pressure-loving. [00...

AI Technical Summary

Problems solved by technology

[0003] The period between sampling and culturing on the scientific research ship is long. If high-pressure equipment is not used, strictly barophilic microorganisms may be lost during this period, which affects our analysis of in situ ecosystems and the isolation of strictly barophilic microorganisms.

In addition, traditional high-pressure culture equipment is bulky, complex in structure, and high in cost, and is not suitable for simultaneously cultivating multiple biological samples in large quantities.

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