78 results about "Resistant fungi" patented technology

An agri-horticultural fungicide containing a substituted isoxazole alkylamine derivative represented by the formula (I) as an active ingredient exhibits no crossreaction with known drug resistant fungi and exerts a sufficient activity for preventing plant disease damages: wherein R1 and R2 each represent a hydrogen atom, a (substituted) alkyl, or the like; X is a single bond, alkylene, or the like; Y1 represents a (substituted) lower alkyl, (substituted) cycloalkyl, (substituted) phenyl, (substituted) heteroaryl, or the like; Y2 represents a (substituted) cycloalkyl, (substituted) phenyl, (substituted) heteroaryl, or the like; and Z represents a (substituted) alkylene, —O-((substituted)alkylene)-, —NR-((substituted)alkylene)- or the like.

The invention discloses a method for harmless treatment of ferment antibiotic fungus residues. The method includes: subjecting the ferment antibiotic fungus residues to ultrahigh-temperature intensive acidogenic fermentation treatment; performing biochemical treatment on the fungus residues subjected to ultrahigh-temperature intensive acidogenic fermentation treatment. Thermophilic hydrolysis acidogenic bacteria are adopted for high-temperature anaerobic acidogenic fermentation treatment of the ferment antibiotic fungus residues, the fungus residues are hydrolyzed and acidized, antibiotic producing strains in the fungus residues are inactivated, residual antibiotics are degraded prior to biochemical treatment, and accordingly harmless treatment and recycling of the antibiotic fungus residues are realized. By adoption of the method, the antibiotic producing strains in the fungus residues subjected to harmless treatment are completely killed, antibiotic residues are avoided, and the fungus residues subjected to biochemical treatment is free of dangers caused by drug-resistant fungi and drug-resistant genes. The method has advantages that efficiency in harmless treatment of the antibiotic fungus residues is improved, environment protection is benefited, hazardous wastes are recycled, and remarkable economic benefits and social benefits are achieved.

The invention relates to the technical field of medicaments, in particular to novel application of kaempferol as a synergist of anti-fungal medicaments. The anti-fungal medicaments are azole-type or polyene-type anti-fungal medicaments and based on the effective concentration of the anti-fungal medicaments, the adding ratio of the kaempferol is 0.5 to 16 mu g / ml. Tests show that when the kaempferol and the anti-fungal medicaments such as fluconazole, ketoconazole, miconazole and amphotericin B are used together, not only the anti-fungal effect is guaranteed on the premise of lowering the consumption of the anti-fungal medicaments, but also the anti-fungal medicaments can restore the function of killing the drug-resistant fungi, so that the kaempferol can be used as a synergist of the anti-fungal medicaments. The kaempferol used as the synergist of the anti-fungal medicaments can lower the consumption of the azole-type or polyene-type anti-fungal medicaments so as to reduce the toxic and side effects of the medicaments; and the kaempferol can make the anti-fungal medicaments restore the function of killing the drug-resistant fungi, so that the kaempferol can effectively treat mycotic infection, particularly drug-resistant t mycotic infection, and has quite important clinical application values.

The invention discloses antifungal application of fraxetin, belonging to the technical field of medicines. In-vitro experiments show that fraxetin has relatively good inhibition effect to the fungi, is capable of restoring the effect of an antifungal drug, namely fluconazole to drug-resistant fungi and therefore can be taken as the antifungal drug and a synergist for the antifungal drug. Accordingto the antifungal application, the new use of fraxetin is opened up, fraxetin is used as the antifungal drug and the synergist for the antifungal drug, a new candidate drug is provided for the fungustreatment, and the antifungal effects of existing drugs are improved. Under the conditions that clinical fungus drug resistance becomes common increasingly and the drug resistance level becomes serious increasingly, the effect of an antifungal drug to the drug-resistant fungi is restored, and the use dosage of the antifungal drug is reduced, so that the medical expense of a patient is saved, andthe toxic and side effects of the drugs are reduced.

The invention discloses a method for remedying cadmium-contaminated soil through turfgrass and microorganism combination, and relates to a heavy metal-contaminated soil treatment technology. The method comprises the following steps: (1) screening cadmium-resistant bermuda grasses so as to obtain the bermuda grasses with strong cadmium resistance; (2) collecting rhizosphere soil of the bermuda grasses in a contaminated area; (3) screening cadmium-resistant microorganisms so as to obtain the microorganisms with the strongest cadmium resistance, which are fungi-aspergillus aculeatus; (4) preparing microorganism remedying matrixes; and (5) carrying out turfgrass and microorganism combined remediation. The method has the advantages that the dominant plants, namely the bermuda grasses in the cadmium-contaminated area are used as cadmium-resistant plant screening sources and have strong adaptability to the soil environments in the contaminated area; the cadmium-resistant fungi-aspergillus aculeatus is derived from the rhizosphere soil of the bermuda grasses in the cadmium-contaminated area, is well compatible with the cadmium-resistant bermuda grasses and has strong adaptability to the soil environments in the contaminated area; the microorganism remedying matrixes can be used for ensuring the nutrient supply of microorganism growth, enhancing the operability of applying the microorganisms in soil and improving soil environments, and the waste is effectively utilized; the method is low in cost and simple and convenient to operate.

The invention discloses a novel application of honokiol and fluconazole in antifungal cooperation. According to the invention, the fungi are candida albicans. According to the invention, a standard macrodilution drug sensitivity experiment recommended by CLSI is adopted, in vitro sensitivity of honokiol towards clinically separated drug resistant candida albicans is evaluated with systematic methods such as a checkerboard macrodilution method and a time-kill curve method. Also, an in vitro synergistic effect of honokiol and fluconazole in clinic drug resistant fungi resistance is evaluated. Further, with a mice infection and treatment experiment, an in vivo synergistic effect of honokiol and fluconazole in clinic drug resistant fungi resistance is evaluated.

The invention discloses a compound halophilous-microorganism bacterium agent and an application thereof. Halophilous microorganisms are the microorganisms which are not inhibited or poisoned by high-concentration inorganic salts in the high-salt environment, can carry out normal growth and metabolism and comprise salt-resistant microorganisms and halophilic microorganisms. The compound halophilous-microorganism bacterium agent at least comprises two of halophilic bacteria, halophilic archaea, salt-resistant bacteria, salt-resistant archaea, halophilic fungi and salt-resistant fungi, wherein the types and the quantity of the halophilous microorganisms are at least 10, and the halophilous microorganisms at least comprise one type of uncultured microorganisms. The compound halophilous-microorganism bacterium agent disclosed by the invention has the advantages that high-efficiency and stable degradation can be carried out on organic matters under the condition of 1%-20% salt; by using the compound halophilous-microorganism bacterium agent for biochemical treatment of high-salt waste water and microorganism restoration of saline-alkaline soil, the pollution treatment cost can be reduced.

The invention discloses a method for carrying out ecological remediation in cadmium-contaminated soil by using cadmium resistant fungi, and relates to a microorganism ecological remediation technology. The method comprises the following steps: (1) separating and purifying cadmium resistant fungi; (2) identifying the cadmium resistant fungi; (3) determining the most suitable growth condition of the cadmium resistant fungi; (4) preparing cadmium resistant fungus remediation matrixes; and (5) remedying the cadmium-contaminated soil. The method has the advantages that rhizosphere soil of dominant plants in a cadmium-contaminated area is used as a cadmium resistance microorganism screening source, and the defect of the adaptation of cadmium resistance microorganisms screened out to the soil environments in the remedied area is overcome; bermuda grasses which have a certain capacity of resisting cadmium and are compatible with the cadmium resistant fungi are used for overcoming the repulsive interaction between plants and microorganisms; the cadmium resistant fungi are inoculated in saw dust for application, so that the nutrient supply of microorganism growth is ensured, and the waste is effectively utilized; the method is low in cost, simple and convenient to operate, and the microorganisms rapidly grow and can breed on a large scale.

The invention discloses a medicinal composition for treating mycotic infection at the superficial part and application thereof. The medicinal composition comprises miconazole and hanfangchin A, wherein the concentration of the hanfangchin A in a miconazole and hanfangchin A combined system is more than or equal to 40mug / ml. The medicinal composition can be used for an active component for preparing a medicament for treating the mycotic infection at the superficial part. After being verified by an in-vitro antimicrobial test, the hanfangchin A can be used for enhancing the antimycotic effect of the miconazole on dermatophyte and monilial to different degrees, in particular bacteriostasis effect on medicament-resistant fungi; and meanwhile, the dosage of the miconazole is reduced.

The invention discloses a pretreatment method of ferment antibiotic fungi residues. The pretreatment method comprises ultrahigh temperature reinforced acidogenic fermentation treatment of the ferment antibiotic fungi residues. Thermophilic hydrolysis acid-forming bacteria are used for performing high temperature anaerobic digestion treatment on the ferment antibiotic fungi residues, so that the fungi residues are hydrolyzed and acidified, antibiotic producing bacteria in the fungi residues are inactivated, and retained antibiotics are degraded. The fungi residues pretreated through the method can be directly subjected to subsequent biochemical treatment after being subjected to antibiotic removal. According to the method disclosed by the invention, the antibiotic producing bacteria can be completely killed, the antibiotics in the fungi residues can be removed, inhibition of high-concentration antibiotics to microorganisms is reduced, the difficulty in treatment of the fungi residues based on the subsequent biochemical method is reduced, generation of drug-resistant fungi and drug-resistant genes in the subsequent biochemical treatment is reduced, the harmless treatment efficiency of the antibiotic fungi residues is improved, and environmental protection is facilitated.

The invention relates to the field of biopesticide preparation research and development, and particularly relates to a tetramycin compound and application, which is the divisional application, wherein the application number is 2013106143470, the application date is Nov, 26th, 2013, and the invention title is tetramycin compound and application. The tetramycin compound comprises tetramycin and active substances, wherein the active substances are pyrroles or copper compounds. Compared with the prior art, according to the tetramycin compound and application which are provided by the invention, the danger of selectivity of resistant fungi strains is reduced; the service lives of the active components are shortened; the prevention and treatment range is expanded; the disease prevention effect is improved; the drug resistance of germs can be slowed down or overcome; the cost is reduced, and the application times of the pesticide is reduced.

The invention belongs to the field of medicines and in particular relates to HRP5 analogues and a preparation method thereof. The general formulas of amino acid sequences of the HRP5 analogues disclosed by the invention are shown in SEQ ID NO:2, namely Xaa1-Xaa2-Xaa3-Xaa4-Xaa5-Xaa6-Xaa7-Xaa8-Xaa9-Leu-Xaa11-Lys-Tyr-Leu-Arg-Xaa16-Xaa17-Xaa18. The in-vitro activity measurement proves that the polypeptides provided by the invention have obvious effects of killing gram-positive bacteria, Gram-negative bacteria and fungi, also have obvious effects of killing clinically separated drug-resistant gram-positive bacteria, drug-resistant gram-negative bacteria and drug-resistant fungi and have the characteristics of broad spectrum and high efficiency.

The invention relates to a microorganism / animal and plant combined ecological restoration method for farmland soil cadmium pollution. The method comprises the following steps: introducing cultured adult earthworms into farmland cadmium polluted soil, and adding crushed fine baits; introducing cadmium-resistant fungi one month before sexual maturity of adult earthworms while sowing ryegrass; harvesting the aboveground part of the ryegrass, and treating; and arranging a plastic greenhouse constant-temperature humidity control device for farmland remediation soil, controlling the indoor temperature of a greenhouse to be 15-20 DEG C, and keeping the soil humidity to be 20-30%. The method has the advantages that adult earthworms can resist 1.5-2.4 mg / kg of cadmium pollution, improve the soil fertility and porosity, promote the breeding of cadmium-resistant fungi and facilitate the root development of ryegrass; the cadmium-tolerant fungi can slow down the toxic action of cadmium pollution onindigenous microorganisms and plants and enhance the transfer of cadmium ions to the roots of ryegrass; and the ryegrass enriching soil cadmium pollution is treated to permanently remove cadmium. Themethod is low in investment, ecological, environment-friendly, safe, harmless and effective for a long time.

The invention discloses use of rhizoma paridis chinensis for preparing an antifungal drug, belonging to the technical field of medicines. In-vitro cell experiments show that rhizoma paridis chinensishas different levels of antifungal effects and even fungus-killing effect to drug-resistant candida albicans 103, candida albicans SC5314, cryptococcus neoformans, candida krusei, candida parapsilosis, candida glabrata and candida tropicalis and therefore can be taken as the antifungal drug. According to the use, new use of rhizoma paridis chinensis is opened up, rhizoma paridis chinensis is usedfor preparing the antifungal drug, and a new candidate drug is provided for the treatment of fungal infection. Currently, drugs with wide antifungal spectrums and fungus-killing activity on clinic arevery few, and a new way is provided for the clinical and efficient treatment of infection of fungi and even drug-resistant fungi by virtue of the characteristics of wide antifungal spectrums and fungus-killing activity of rhizoma paridis chinensis under the conditions that clinical fungus drug resistance becomes common increasingly and the drug resistance level becomes serious increasingly.

The invention relates to the fields of gene engineering and fermentation engineering, and specifically relates to an acid resistant fungi alpha-amylase TaAMY, gene and applications thereof. The invention provides a novel acid resistant fungi alpha-amylase TaAMY, which has an amino acid sequence represented by the SEQ ID No.1, also provides a gene NTaAMY, which encodes the novel acid resistant fungi alpha-amylase mentioned above and has a nucleotide sequence represented by the SEQ ID No.2, and also provides a recombinant vector containing the gene mentioned above, a recombinant bacterium strain and applications thereof. The novel acid resistant fungi alpha-amylase can reach a relatively high fermentation level after the high-level expression, and can be widely applied to fields of starch sugar making, brewing, baking, medicine, and feed.

The invention relates to the field of medicine technology and is especially new use of shikonin as an antifungal medicine synergist. The antifungal medicine comprises azole or polyene antifungal medicine and the like, the charge of shikonin is 1-16 mu g / ml in the antifungal medicine. Experiments show that when shikonin is used with the antifungal medicines such as fluconazole, ketoconazole, miconazole or amphotericin B and the like, the treatment effect of superfacial or deep fungal infection can be ensured under the circumstances of reduction of dosage, and the effects of the antifungal medicines to resistant fungi are recovered, thereby that shikonin can be used as antifungal medicine synergist with the advantages of reducing dosages of azole or polyene antifungal medicine so as to reduce side effects of medicines, especially recovering the effects of the antifungal medicines to resistant fungi to treat resistant fungal infection, and having important clinical application value.

The invention relates to the technical field of medicaments, in particular to application of China rose flower in preparation of an antifungal product. Experiments in vitro show that the antifungal product has certain inhibiting effect on growth of medicament resistant fungi and other fungi. Therefore, the China rose flower can be applied to preparation of the antifungal product.

The invention discloses a baicalein derivative, the structure of which is shown in the specification, in the formula, R1 is hydrogen, alkyl, or substituted / unsubstituted aryl; R2 is hydrogen, halogen,alkyl, alkoxy, substituted / unsubstituted amino, or substituted / unsubstituted aryl; R3 is oxygen or sulfur; and R4 is a substituted / unsubstituted aryl group. The synthesis method of the baicalein derivative is relatively simple and convenient; pollution is reduced, experiments prove that the prepared baicalein derivative can be used together with azole antifungal drugs, the sensitivity of drug-resistant bacteria to the azole drugs can be improved, that the drug resistance is reversed, and a novel way is provided for the treatment of drug-resistant fungi in clinic.

The invention relates to the technical field of medicaments, in particular to novel application of sodium houttuyfonate as a synergist of an antifungal medicament. The antifungal medicament is an azole or polyene antifungal medicament; and 1 to 16mu g of sodium houttuyfonate is added into each 1ml of antifungal medicament. Experiments prove that: when the sodium houttuyfonate is combined with the antifungal medicament such as fluconazole, ketoconazole, miconazole or amphotercin B, and the like, the treatment effect of the sodium houttuyfonate on superficial or deep fungal infection can be ensured under the condition of reducing the dosage and the sodium houttuyfonate can make the antifungal medicament recover the effect on medicament-resistant fungi, so the sodium houttuyfonate can be used as the synergist of the antifungal medicament. The sodium houttuyfonate serving as the synergist of the antifungal medicament can reduce the dosage of the azole or polyene antifungal medicament so as to reduce the toxic and side effects of the medicament; and particularly, the sodium houttuyfonate makes the antifungal medicament recover the effect on the medicament-resistant fungi, effectively treats fungal infection, particularly medicament-resistant fungal infection, and has important clinical application value.

The invention relates to the field of medicines, and particularly relates to an HRP5 analogue and a preparation method thereof. The general formula of the amino acid sequence of the HRP5 analogue is as shown in SEQ ID NO:2: Xaa1-Xaa2-Xaa3-Xaa4-Xaa5-Xaa6-Xaa7-Xaa8-Xaa9-Leu-Xaa11-Lys-Tyr-Leu-Arg-Xaa16-Xaa17-Xaa18. The activity determination of the HRP5 analogue in vitro shows that the polypeptide provided by the invention has significant sterilization effects on gram positive bacteria, gram negative bacteria and fungi, also has significant sterilization effects on drug-resistant gram positive bacteria, drug-resistant gram negative bacteria and drug-resistant fungi clinically isolated, and has the characteristics of being wide in spectrum and high in effects.

The invention provides a marine oligosaccharide-containing antifungal infection composition for pets, and belongs to the technical field of animal medicines. The invention opens up a new application of guluronic acid oligosaccharide, and the guluronic acid oligosaccharide serving as a synergist of antifungal drugs for pets can reduce the dosage of antifungal drugs such as azoles, polyenes and thelike, so that the toxic and side effects of the drugs, especially fluconazole, nystatin, amphotericin B and the like, are reduced. Especially, the guluronic acid oligosaccharide serves as a synergistof the antifungal drug for pets, so that the antifungal drug recovers the effect on drug-resistant fungi, pet fungal infection, especially drug-resistant fungal infection, is effectively treated, thetoxicity of the drug is reduced, the economic burden of pet medication is relieved, and the guluronic acid oligosaccharide has important feeding application value.

The invention relates to bis-substituted aryl compounds and application thereof. The structure of the bis-substituted aryl compounds is disclosed as Formula I, II or III. The experimental verification detects that the bis-substituted aryl compounds can be combined with azole antifungal drugs to enhance the sensitivity of drug-resistant fungi to azole drugs and implement drug resistance reversion. Therefore, the invention provides a new way for clinical treatment of drug-resistant fungi.

The invention provides an application of anthraquinone compound in preparing an antifungal drug and an antifungal composition, which belongs to the technical field of medicine. The anthraquinone compound is 9,10-dioxo-N-(5-phenylthiazole-2-groupyl)-9,10-dihydroanthracene-2-sulfonyl; and an in vitro cell experiment proves that the anthraquinone compound has good antifungal effect for various clinical fungi strains, the application dosage of the antifungal drug can be obviously reduced, the inhibition effect for the drug-resistant fungi can be improved, and the effect of the antifungal drug for the drug-resistant fungi can be restored.

The invention discloses an application of polygonum cillinerve in fungus resisting, and belongs to the technical field of medicine. In vitro experiments show that polygonum cillinerve has a good inhibitory effect on fungi, and can restore the effect of antifungal drug fluconazole on drug-resistant fungi. Therefore, the polygonum cillinerve can be used as an antifungal drug and a synergist of the antifungal drug. The invention opens up a new use for the polygonum cillinerve, uses the polygonum cillinerve for antifungal drugs and synergists of the antifungal drugs, not only provides s new drug candidate for fungal treatment, but also improves the antifungal action of existing drugs. In the case of the increasing prevalence of clinical fungal resistance and the increasing degree of drug resistance, the polygonum cillinerve restore the effect of antifungal drugs on drug-resistant fungi, and reduces the dosage of antifungal drugs, thereby saving medical expenses for the patient and reducingthe toxic side effect of the antifungal drugs.

The invention belongs to the field of enzyme preparations and discloses a variable-temperature production method for high-temperature-resistant fungi alpha-amylase. By gradient cooling and gradient heating type fermentation, anti-stress performance of 901-18Trichoderma reesei 901-18 is remarkably improved, enzymatic productivity of mixed cultures is presented maximally, and the high-temperature-resistant fungi alpha-amylase produced according to the method have the advantages of high enzyme activity, extensive action conditions, high stability and suitableness for industrial production.

Biopolymer compositions comprising antimicrobial peptides (AMPs) for treating infections such as bacterial infections, viral infections, fungal infections, and parasitic infections. The compositions herein may also be used for treating infections associated with antibiotic-resistant bacteria, antifungal-resistant fungi, antiviral-resistant viruses, or for treating biological warfare agents (BWAs) such as Bacillus anthracis and Yersenia pestis. The present invention also provides methods of synthesis of said biopolymer compositions, wherein AMP biopolymers can be synthesized as an artificially engineered protein by genetically fusing an AMP; a protein that behaves similarly to polymer tethers; and a protein as a modifiable material platform that can transform to self-assembled nanoparticles, self-standing films, or adhesives to easily attach tethered AMPs onto any biomaterial surface for various clinical applications.

The invention relates to the fields of search and development of biopesticide preparations, and specifically relates to a tetramycin compound and an application. The invention is the divisional application applied on 26th, November, 2013 with the application number of 2013106143470, and is named tetramycin compound and an application. The tetramycin compound is prepared from tetramycin and active substances, wherein the active substances are esters, including one or a plurality of the following substances: dithiocarbamate, carbamate and pyrethroid. Compared with the prior art, the tetramycin compound and an application have the advantages that the danger of resistant fungi strain selection is reduced, the service lives of the active components are prolonged, and the prevention and control scope is expanded; the disease preventing effect is improved; the drug resistance of bacteria can be delayed or overcome; the cost is saved; and the times of application of pesticides can be reduced.

The present invention relates to an antibacterial and fungicidal peptide in which a lysine and tryptophan dipeptide is repeated. More specifically, the antibacterial and fungicidal peptide of the present invention, in which lysine and tryptophan dipeptide is repeated four times, shows excellent antibacterial activities with respect to gram-positive bacteria, gram-negative bacteria and antibiotic-resistant strains by affecting the inner membrane of harmful microorganisms, has remarkable fungicidal activities with respect to pathogenic fungi and antibiotic-resistant fungi, and shows little cytotoxicity, and thus can be useful for a pharmaceutical composition, a cosmetic composition, agricultural chemicals, a food preservative, a cosmetic preservative, and a pharmaceutical preservative.

The invention relates to the technical field of medicines and discloses usage of Chinese roses for preparing an anti-fungal drug synergist product. By the aid of in vitro experiments, Chinese rose extract is applied in combination with antifungal drugs such as fluconazole, itraconazole and ketoconazole, so that therapeutic effects of superficial and deep fungal infection can be improved to different extents, and effects of anti-fungal drugs on drug-resistant fungi are restored. Therefore, Chinese roses can serve as the anti-fungal drug synergist product.

The invention belongs to the technical field of biological medicines, and discloses a pharmaceutical composition with drug-resistant fungi resistance, which is composed of panax stipuleanatus n-butylalcohol extract and antifungal drugs. The panax stipuleanatus n-butyl alcohol extract is obtained by cleaning panax stipuleanatus, drying the panax stipuleanatus in the sun, crushing the panax stipuleanatus, extracting the panax stipuleanatus with n-butyl alcohol and distilling under reduced pressure. The panax stipuleanatus n-butyl alcohol extract can obviously reduce the efflux function of a fungal cell efflux pump, so that the expression level of drug resistance related genes is obviously reduced, and the mitochondrial membrane potential level in fungal cells is reduced; the pharmaceuticalcomposition composed of the panax stipuleanatus n-butyl alcohol extract and the antifungal drug has a good inhibition effect on drug-resistant fungi.