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Chinese rose is used to prepare the purposes of antifungal drug synergist product

Technology of an antifungal drug, rose flower, applied in the field of medicine

Inactive Publication Date: 2015-08-12
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on its combined use with antifungal drugs for antifungal

Method used

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  • Chinese rose is used to prepare the purposes of antifungal drug synergist product
  • Chinese rose is used to prepare the purposes of antifungal drug synergist product
  • Chinese rose is used to prepare the purposes of antifungal drug synergist product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Example 1: The effect of Chinese rose and fluconazole in combination on different clinical fungal strains

[0010] Materials and methods

[0011] 1. Test drug:

[0012] The medicinal raw material of Chinese rose is the dried flower of Rosaceae rose. Extraction process: Add the crushed medicinal material powder into a 1000ml round bottom flask, add 70% ethanol in a water bath and reflux for 2 hours, filter the reflux liquid, put it into a weighed flask, and rotate it under reduced pressure at about 50°C until it becomes a viscous extract . Add distilled water in the flask, heat to fully dissolve (take out a little as the water extract to obtain a crude drug concentration of 1g / ml), then add anhydrous ethanol equivalent to distilled water, heat and mix thoroughly, and combine the extracts and mix to obtain Take a little of the crude drug concentration of 0.5g / ml into EP tubes for in vitro experiments.

[0013] Fluconazole injection: Pfizer Pharmaceutical Co., Ltd., b...

Embodiment 2

[0042] Example 2: Combination of Chinese rose and other azole antifungal drugs

[0043] Materials and methods

[0044] 1. Drugs

[0045] Chinese rose extract: extraction steps and methods are the same as in Example 1

[0046] Itraconazole: purchased from Sigma, USA

[0047] Ketoconazole: purchased from Sigma, USA

[0048] Itraconazole and ketoconazole dimethyl sulfoxide were formulated at 6.4 mg / ml, and each reagent was stored at -20°C. Before the experiment, the drug was taken out and placed in a 35°C incubator to melt, mixed well, and pharmacodynamic tests were carried out respectively.

[0049] 2. Other experimental steps and methods are the same as in Example 1

[0050] The experimental results are shown in Table 3

[0051] Table 3 The MIC80 value (μg / ml) of Chinese rose extract and other antifungal drugs against clinical drug-resistant Candida albicans 100

[0052]

[0053] It can be seen from Table 3 that the MIC of miconazole, ketoconazole and itraconazole ag...

Embodiment 3

[0054] Example 3: Protective effect of Chinese rose combined with fluconazole on systemic fungal infection in mice

[0055] Test design:

[0056] 50 ICR mice were injected with 1 × 10 7 CFU / ml logarithmic growth phase Candida albicans suspension 0.2ml, replicate systemic mouse deep fungal infection model, and randomly divide into the following 5 groups: model group, fluconazole 0.025mg / ml group, fluconazole group Azole 0.025mg / ml+ rose extract 100mg / ml group, fluconazole 0.025mg / ml+ rose extract 20mg / ml group, fluconazole 0.025mg / ml+ rose extract 4mg / ml group.

[0057] Two hours after modeling, the drug was administered by intragastric administration, with a volume of 0.2ml / 10g, once a day for four consecutive days. The mice were observed for death within 10 days after the start of administration.

[0058] statistical methods:

[0059] Kaplan-Meier survival analysis, statistical software SPSS17.0.

[0060] Experimental results

[0061] The effect of Chinese rose combin...

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Abstract

The invention relates to the technical field of medicines and discloses usage of Chinese roses for preparing an anti-fungal drug synergist product. By the aid of in vitro experiments, Chinese rose extract is applied in combination with antifungal drugs such as fluconazole, itraconazole and ketoconazole, so that therapeutic effects of superficial and deep fungal infection can be improved to different extents, and effects of anti-fungal drugs on drug-resistant fungi are restored. Therefore, Chinese roses can serve as the anti-fungal drug synergist product.

Description

Technical field: [0001] The invention relates to the technical field of medicine, and relates to the use of rose flowers for preparing synergist products of antifungal drugs. Background technique [0002] Chinese rose is the flower of Rosaceae Rosa plant, and its roots and leaves are also used as medicine. Rose flowers are sweet in taste and warm in nature, enter the liver meridian, and have the effects of promoting blood circulation, regulating menstruation, reducing swelling and detoxification. Due to the obvious effects of removing blood stasis, promoting qi and relieving pain, rose flower is often used to treat irregular menstruation, dysmenorrhea and other diseases. There is no report on its combined use with antifungal drugs for antifungal. Contents of the invention [0003] The invention provides an application of rose flower for preparing antifungal drug synergist products. It has been confirmed by in vitro cell experiments that it has obvious synergistic effect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/738A61P31/10A61K133/00
Inventor 曹永兵林辉刘伟顾文文王耘安毛毛姜远英
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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