471 results about "Antifungal drug" patented technology

An alkyl substituted triazazole type antifungal compound for preparing the antifungal medicines, and its various enantiomers, diaisomers, Nox and precursors are disclosed.

The invention relates to a quick diagnostic kit used for testing four or more than four kinds of invasive candidas and an application method thereof. The quick diagnostic kit is characterized in that the kit is internally provided with fluorescent quantitative PCR reaction liquid containing specific primers and fluorescent probes corresponding to the candidas which are at least four kinds as well as positive control materials and negative control materials of the candidas which are at least four kinds; Ct value tested by a fluorescent quantitative PCR tester is used for judging. The kit has the characteristics of good specificity and high sensitivity; due to double control of high specificity amplification of the primers and high specificity hybridization of the fluorescent probes, the false positive is low; therefore, the invention can be used for simultaneously testing four kinds of invasive candidas which have different sensitivities for clinical first-line antifungal drugs such as fluconazole and the like in a simple, convenient, rapid, special and sensitive way.

The invention relates to an extracellular antibacterial lipopeptide of plant endophytic Bacillus subtilis Jaasedl and a separation and purification method. The distribution range of the molecular weight of the extracellular antibacterial lipopeptide is mainly between 1,000Da and 2,200Da. The extracellular antibacterial lipopeptide contains an Iturin homologue, a Fengycin homologue and a Surfactin-like Compound homologue. The extracellular antibacterial lipopeptide is a group of uncommon antibacterial lipopeptide mixture produced by a single bacterial strain. The separation and purification method of the extracellular antibacterial lipopeptide comprises the following steps of: after salting out to obtain the crude extract of the antibacterial lipopeptide by using ammonium sulfate from the fermentation liquor of the endophytic Bacillus subtilis Jaasedl, performing Sephedex G-25 molecular sieve chromatography, Cellulose DEAE-52 anion exchange chromatography and FPLC300SB-C18 column chromatography successively, wherein 34 to 37 min of collecting peak has bacteriostatic activity; detecting by Tricine-SDS-PAGE after concentrating; and achieving electrophoretically pure at only one strip to obtain pure extracellular antibacterial lipopeptide. The antibacterial lipopeptide has extremely high research and application value for the development of broad-spectrum antifungal medicaments, and has wide application prospect for natural quality protection of crops and pesticide residue reduction.

The invention discloses a purification method of echinocandins antifungal drug anidulafungi. The method comprises the following steps of: (1) preparing a dry sample: adding organic solvent in a crude product of the anidulafungin to dissolve the crude product, adding silica gel after the crude product of the anidulafungin is adequately dissolved, and uniformly mixing and drying the mixture to obtain the anidulafungin dry sample; (2) pressurizing and eluting: uniformly filling the anidulafungin dry samples in the top end of a chromatographic column with silica gel, pressurizing and eluting the chromatographic column with the anidulafungin dry samples by adding elution solvent, utilizing a high-effective liquid phase chromatography to monitor, and collecting the elution solution with the anidulafungin content being greater than 98 percent; and (3) concentrating: concentrating the elution solution with the anidulafungin content being greater than 98 percent until dryness to obtain a pure product with the anidulafungin content being greater than 98 percent. By adopting the column chromatography, simplicity in operation is realized, and the equipment cost is low; the organic solvent with low toxicity and low boiling point is adopted as the elution solution, so that the subsequent recycling treatment is simple, and the environmental pressure can be greatly reduced; and the purification separation time is short, the purification effect is good, the purification yield is high, and the purification method is applicable to industrialized mass production.

The invention relates to a novel broad-spectrum antifungal medicine compound, a broad-spectrum antifungal medicine composition, an application of the compound or the composition in the preparation of a broad-spectrum antifungal medicine, and an application of the compound or the composition in the preparation of a medicine used for treating severe invasive infection (comprising Candida krusei) caused by invasive aspergillosis and / or Fluconazole-resistant Monilia and / or severe infection caused by Scedosporium and fusarium. The novel broad-spectrum antifungal medicine compound and the broad-spectrum antifungal medicine composition have extensive and strong antifungal activity and better dynamic property and safety. For deep mycotic infection, the novel broad-spectrum antifungal medicine compound and the broad-spectrum antifungal medicine composition are better than the original voriconazole in the aspects of the antibiotic activity and the medicine resistance and is better than amphotericin B in the aspects of safety and effectiveness; and compared with the formerly applied voriconazole, Fluconazole, itraconazole and amphotericin B, the novel broad-spectrum antifungal medicine compound and the broad-spectrum antifungal medicine composition have reinforced medicine effect, less adverse reaction and good safety.

The invention relates to a triazole alcohol derivative and a preparation method and application thereof. The chemical structure of the triazole alcohol derivative is as shown in the formula I. The invention also provides salt of the compound, a pharmaceutical composition, a preparation method and application. The compound of the invention has strong antifungal activity, has advantages of low toxicity and wide antimicrobial spectrum, and can be used for preparation of antifungal drugs.

The invention relates to carbonyl reductase, a mutant and application thereof in preparation of antifungal drug intermediates. The invention specifically discloses xylose fermentation yeast carbonyl reductase and a mutant with improved activity thereof, encoding genes and amino acid sequences, recombinant expression vectors and recombinant expression transformants containing the gene sequences, as well as application of catalyzing asymmetric reduction of prochiral carbonyl compounds by taking the xylose fermentation yeast carbonyl reductase or corresponding recombinant expression transformants as catalysts, particularly catalyzing reduction of 2-chloro-2',4'-difluoroacetophenone and 2,2',4'-trichloroacetophenone for preparing antifungal compound precursors (R)-2-chloro-1-(2',4'-difluorophenyl)ethanol and (R)-2-chloro-1-(2',4'-dichlorophenyl)ethanol. Compared with the existing other asymmetric reduction methods, the method disclosed by the invention has the advantages of high enzymatic reaction substrate concentration, mild reaction condition, environment friendliness, high yield, high optical purity of products and the like and has excellent application prospects.

The invention provides a nitrogen methyl side chain-substituted triadimenol antifungal compound and a preparation method thereof, namely a 1-(1H-1, 2, 4-triazole-1-yl)-2-(2, 4-difluoro phenyl)-3-(N-methyl side chain-substituted amino)-2-propyl alcohol compound (comprising a racemate thereof, and an R type or S type isomer), and hydrochloride, nitrate, hydrobromide and methane sulfonate and a preparation method of the compound. The compound has a strong antifungal effect on various shallow and deep fungi; and compared with the traditional antifungal medicament in clinical practice, the compound has the advantages of high efficiency, low toxicity and wide antifungal spectrum and can be used for preparing new antifungal drugs. The structural formula of the compound is shown as follows.

The invention discloses a Paliurus ramosissimus extract with antifungal activity and preparations and applications thereof. The preparation method includes the steps of extracting terpenoids, flavonoids, alkaloids, cumarins (including glycosides and monomers of terpenoids, flavonoids, alkaloids and cumarins) and polysaccharides from a whole plant or any part of Paliurus ramosissimus with solvent, collecting extractive solution, concentrating and drying to obtain Paliurus ramosissimus extract; making into tablets, granules, ointment, gel, film, liniment, lotion and spray. The Paliurus ramosissimus extract with antifungal activity and preparations disclosed by the invention are used for preparing antifungal drugs.

The invention provides a glycopeptide antifungal compound and a pharmaceutically acceptable salt thereof, a preparation method and application thereof, and a pharmaceutical composition containing the active component. The glycopeptide antifungal compound has a general structural formula shown as below; n equals to 1, 2 or 3; and R represents monosaccharide or disaccharide, wherein the monosaccharide is glucose, galactose, xylose, rhamnose, mannose, ribose, glucosamine or acetyl glucosamine, and the disaccharide is maltose or lactose. Compared with existing clinical antifungal drugs, the compound provided by the invention has advantages of high efficiency, low toxicity and broad antibacterial spectrum, etc.; therefore, the compound can be applied to preparation of antifungal drugs. The preparation method provided by the invention successfully introduce different glycosyls onto primary amino group of Caspofungin, and has advantages of simple process and high yield.

A topical treatment for skin disorders and diseases comprising a combination of at least one antifungal agent and at least one hydroxy acid agent having a pH−pKa value of 0.5 or more formulated into shampoos, creams, lotions, gels, sprays, foams, pads, films, patches, and solutions for treatment of skin disorders and diseases in both humans and animals.