62 results about "Protein staining" patented technology

The invention relates to the technical field of daily chemical washing products, in particular to a use of a polymer as a protease stabilizing agent in a detergent and a detergent composition containing the polymer. Ethoxylation polymine disclosed by the invention is used as the protease stabilizing agent in the detergent, so that the protease stability in the detergent can be promoted, and the dirt removing properties of protease can also be strengthened. The detergent composition containing the ethoxylation polymine and the protease disclosed by the invention is high in protease stability and good in dirt removing properties, the compatibility of the ethoxylation polymine and a formula and an enzyme preparation is good, the ethoxylation polymine and the enzyme preparation can exert synergistic interaction effects, and further the effect of removing protein stains by the detergent composition can be promoted. The enzyme activity conservation rate of protease in the detergent composition disclosed by the invention is increased by 5% or above compared with the enzyme activity conservation rate of a detergent composition only containing the protease (excluding the ethoxylation polymine).

The invention relates to mass spectrum-compatible protein argentation on electrophoretic gel, which comprises the following steps of: 1) putting gel formed by the electrophoresis of a protein sample in fixing solution for fixing, staining by using staining solution; 2) decoloring by using decoloring solution, washing by using deionized water, and performing sliver staining by using silver nitrate solution; and 3) washing by using the deionized water, developing by using a developing agent, and stopping by using ethylene diamine tetraacetic acid (EDTA) solution. Compared with the conventional protein argentation, the protein argentation provided by the invention has high sensitivity, short required time and high mass spectrum compatibility, and is suitable for the study of high-flux proteomics. The invention relates to the field of protein staining, in particular to the mass spectrum-compatible protein argentation on the electrophoretic gel.

The present invention generally relates to treatment solutions for staining proteins including aluminum ions and staining methods using the same, and more specifically, to a treatment solution for staining proteins including aluminum ions, the treatment solution used when proteins are stained by a Commassie Brilliant Blue CBB stain, and a staining method using the same. The disclosed treatment solution including aluminum ions for CBB-staining proteins can decrease detection limits, thereby improving the sensitivity and reducing staining time as well as omitting a de-staining process.

The present invention discloses a high sensitivity and low background Coomassie brilliant blue (CBB) staining solution and a using method thereof, and belongs to the technical field of protein staining reagents of SDS-PAGE. The staining solution is composed of CBB R250 with a mass volume concentration of 0.03%-0.05%, acetic acid with a volume percentage of 2%-6%, ammonium sulfate with a mass volume concentration of 8%-15%, and ethanol with a volume percentage of 12%-16%. The CBB staining solution provided by the present invention is optimized on the basis of the traditional staining solution,and a clearer background is obtained, so that the detection sensitivity is greatly improved and the staining sensitivity reaches the nanogram level. According to the method for using the CBB stainingsolution provided by the present invention, without elution after staining, deionized water is used to wash to destroy the colloid state to complete decolorization, so that the detection time is greatly shortened, the method can be used for rapid protein staining of SDS-PAGE, and work efficiency can be improved.

Colloidal formulation for staining proteins and methods of their use are provided.

The invention discloses a fluorescent protein dyeing agent as well as a preparation method and application of the fluorescent protein dyeing agent. The structural formula of the fluorescent protein dyeing agent is as shown in the specification. A solution of the fluorescent protein dyeing agent has intense fluorescence, the dyeing agent comprises aldehyde groups which can be reacted with amino on protein to form covalent bonds for dyeing the protein, so that the dyeing agent has the advantages of being firm in dyeing, not liable to decolor, convenient to use, few in operation steps, short in time and high in sensitivity, is capable of improving the dyeing and fluorescence detection effect, and is beneficial to fluorescence or naked-eye recognition and detection.

The present invention discloses an environmental-protection laundry auxiliary agent for a movable laundry room, a preparation method and applications thereof, wherein the laundry auxiliary agent comprises the following raw materials by weight: 22-30 parts of fatty alcohol polyoxyethylene ether, 19-27 parts of polyacrylamide, 5-13 parts of glycerol monostearate, 1-8 parts of jojoba oil, 1-8 parts of nanometer titanium dioxide, and 8-16 parts of sodium acetate. The preparation method comprises: mixing polyacrylamide and deionized water to obtain a polyacrylamide solution; placing nanometer titanium dioxide into the polyacrylamide solution, and carrying out heating stirring to obtain a mixed material; and carrying out mixing heating stirring on fatty alcohol polyoxyethylene ether and a prepared sodium acetate alcohol solution, adding glycerol monostearate and the mixed material, carrying out heating stirring, adding jojoba oil, and carrying out microwave treatment and ultrasonic treatment so as to obtain the product. According to the present invention, the environmental-protection laundry auxiliary agent has advantages of strong detergency, quick effect, less odor and safe use, can perform permeation decomposition removal on various protein stains, human secretions, old blood stains and high temperature treated blood stains, and provides effective effects for washing and maintenance of linen.

A low dose of borax and of liquid detergent is delivered to wash water in a multi-chamber single dose unit containing borax in a powder chamber and a liquid detergent in a liquid chamber. The treatment removes proteinaceous stains without a proteinase enzyme.

The invention discloses a class of anti-tumor compounds containing a triazole heterocyclic structure and an application thereof, belonging to the technical field of medicinal chemistry. The compounds containing the triazole heterocyclic structure introduce quintuple 1,2,3-triazole ring in the form of side chains through the amino condensation, bromination, nitrine substitution, link reaction and other reactions on the basis of naphthalimide parent structure, select tetrazolium salt reduction to test 7721 hepatocellular in the experiment of inhibiting proliferation of tumor cells, and also select sulfonyl rhodamine B protein staining method to test MCF-7 breast cancer cells. The experiment results show that the anti-tumor compounds have wide anti-tumor activity and good inhibition effect on the normal growth of tumor cells derived from different tissues of liver cancer, breast cancer and the like.