62 results about "Protein staining" patented technology

The invention discloses a class of anti-tumor compounds containing a triazole heterocyclic structure and an application thereof, belonging to the technical field of medicinal chemistry. The compounds containing the triazole heterocyclic structure introduce quintuple 1,2,3-triazole ring in the form of side chains through the amino condensation, bromination, nitrine substitution, link reaction and other reactions on the basis of naphthalimide parent structure, select tetrazolium salt reduction to test 7721 hepatocellular in the experiment of inhibiting proliferation of tumor cells, and also select sulfonyl rhodamine B protein staining method to test MCF-7 breast cancer cells. The experiment results show that the anti-tumor compounds have wide anti-tumor activity and good inhibition effect on the normal growth of tumor cells derived from different tissues of liver cancer, breast cancer and the like.

The invention discloses a washing auxiliary agent for removing protein stains, comprising 10-20wt% of fatty alcohol polyoxyethylene ether AEO-9, 10-20wt% of fatty alcohol polyoxyethylene ether AEO-3, 3-5wt% of coconut oil fatty acid diethanolamide, 0.5-2wt% of triethanolamine, 3-5wt% of ethylene glycol butyl ether, 1-5wt% of C12 / C15 fatty alcohol PO-EO block polyether, 5-8wt% of ethanol, 5-10wt% of 2-methyl-2,4-pentanediol, 1-4wt% of sodium citrate, 0.5-1.5wt% of Purafect 4000L liquid alkali protease, 0.5-2wt% of maleate-acrylic acid copolymer, 0.1-0.5wt% of essence, and 30-45wt% of deionized water. The washing auxiliary agent disclosed herein is suitable for pretreatment of linen in professional laundries, can remove blood stain, grass juice, milk, gravy and human body secretion, etc. from the fabric surface, and especially has obvious effect of removing old blood stains. The washing auxiliary agent disclosed herein is good for removing protein stains.

The present invention relates to a method for immobilizing a protein in a sample, which could not easily be immobilized by the conventional immobilization method, to a solid-phase; a method for quantitative determination of protein wherein an effect of inhibitory substance coexisting in a sample prepared using the immobilization method can be reduced; and a rapid and highly precise method for detecting an abnormal PrP and a method for determining BSE using the immobilization method as compared with the conventional method. The present invention provides: “a method for immobilizing a protein to a solid-phase comprising contacting the protein with the solid-phase having hydrophobic surface in the presence of a lower alcohol, and a halogenocarboxylic acid and / or a long chain alkyl sulfate, and an immobilizing reagent solution to be used therefor; a method for quantitative determination of protein comprising contacting a protein-staining solution with the solid-phase immobilized with a protein by the immobilization method, and determining a degree of color development generated thereby; an immunoblotting method wherein the solid-phase immobilized with a protein by the immobilization method is used; and a method for detecting an abnormal PrP a method for determining BSE by using the immobilization method.”

The invention relates to a total-effect clothing pre-washing agent free from an organic solvent. The organic solvent has different degrees of toxic actions to the human body, is combustible and easy to explode, pollutes the environment and has obvious limitation. The total-effect clothing pre-washing agent disclosed by the invention is prepared from an anionic surfactant, a nonionic surfactant, an emulsifier with an HLB value of 5-13, aids, a short chain surfactant, a pH value regulator, a hydrotropic agent, a preservative, essence and water, wherein the emulsifier with the HLB value of 5-13 replaces a common organic solvent and is scientifically compatible with detergents and other aids, oily dirt is removed in emulsifying and dispersing modes, the ratio of the emulsifier to the detergent is between 1:10 and 2:1, an effect of removing multiple stains is achieved, and the stain removal ratios of standard carbon black stained cloth, protein stained cloth and sebum stained cloth are all over 1.0.

There are disclosed metallic colloid particles containing platinum fine particles wherein platinum colloid particles are supported on the surfaces of gold colloid particles, and the platinum fine particles have an average particle diameter of at most 5 nm; a process for producing the metallic colloid particles; a metallic corpuscular carrying body wherein the above metallic colloid particles are supported on a carrier and a process for producing the carrying body. The resultant metallic colloid particles, which have higher sensitivity, are well suited as a label for immunological measurement and protein-staining agent for various proteins.

The invention discloses a natural antibacterial detergent for lady clothes. The preparation method comprises the following steps that: a natural composition is developed which has strong antibacterialactivity on escherichia coli, candida albicans and staphylococcus aureus, namely tea tree essential oil, lemongrass essential oil and ethyl lauroyl arginate HCL; and when the natural antibacterial composition is used for laundry products, the natural antibacterial composition further comprises a surfactant, and the surfactant is cocamidopropyl betaine, lauryl glucoside and disodium cocoyl glutamate. The natural antibacterial detergent for lady clothes is developed on the basis, has super-strong capabilities of removing protein stains, removing blood stains and removing underwear secretion residues, has a high antibacterial rate on escherichia coli, staphylococcus aureus and candida albicans, and has an antibacterial rate of more than or equal to 99.9 percent after acting for 2 minutes; and moreover, the product is clear and transparent in appearance, high in natural component proportion, weakly acidic, mild and non-irritant.

New cleaning compositions and methods of forming and using those compositions are provided. In a preferred embodiment, the compositions are in tablet form, with the tablets being dissolvable in water at the point-of-use for ease of storing and shipping. The compositions comprise sodium bicarbonate, citric acid, an alkali metal alkylbenzene sulfonate, an ingredient selected from the group consisting of a trialkyl glycol monoalkyl ether, limonene, and mixtures thereof, and other ingredients depending upon the embodiment. The diluted cleaner has a very low impact on the environment due to the low levels of surfactants in the diluted solution. Furthermore, the cleaning solution performs well on protein stains due to the production of CO2 and the presence of sodium perborate. The inventive composition can be used on a wide range of substrates, from hard surfaces to fabrics.

The invention relates to the technical field of daily chemical washing products, in particular to a use of a polymer as a protease stabilizing agent in a detergent and a detergent composition containing the polymer. Ethoxylation polymine disclosed by the invention is used as the protease stabilizing agent in the detergent, so that the protease stability in the detergent can be promoted, and the dirt removing properties of protease can also be strengthened. The detergent composition containing the ethoxylation polymine and the protease disclosed by the invention is high in protease stability and good in dirt removing properties, the compatibility of the ethoxylation polymine and a formula and an enzyme preparation is good, the ethoxylation polymine and the enzyme preparation can exert synergistic interaction effects, and further the effect of removing protein stains by the detergent composition can be promoted. The enzyme activity conservation rate of protease in the detergent composition disclosed by the invention is increased by 5% or above compared with the enzyme activity conservation rate of a detergent composition only containing the protease (excluding the ethoxylation polymine).

The invention provides a fabric detergent, a preparation method and application thereof. The fabric detergent comprises thiourea dioxide, a nonionic surfactant, an anionic surfactant, a detergent builder and anhydrous sodium sulphate. The fabric detergent can effectively remove stubborn drug stains on fabrics, can also effectively remove most of protein stains, granular stains, human body fat stains and non-drug-stain pigment stains on medical fabrics, improves the washing efficiency, and saves the washing cost.

Disclosed herein is a method of preparing a protein sample for mass spectroscopy. The method includes separating proteins of the sample on an electrophoresis gel; contacting the proteins with a halo-substituted organic compound; exposing the gel to UV light; detecting fluorescence emitted from the electrophoresis gel; excising at least one portion of the electrophoresis gel based upon the detected fluorescence, wherein said at least one portion contains proteins of the protein sample; and subjecting proteins from the at least one portion to mass spectroscopy. Using this method, more proteins can be identified by GeLC-MS than when the electrophoresis gel is treated with a protein stain or subjected to the gel handling steps accompanying such treatment.

The invention relates to mass spectrum-compatible protein argentation on electrophoretic gel, which comprises the following steps of: 1) putting gel formed by the electrophoresis of a protein sample in fixing solution for fixing, staining by using staining solution; 2) decoloring by using decoloring solution, washing by using deionized water, and performing sliver staining by using silver nitrate solution; and 3) washing by using the deionized water, developing by using a developing agent, and stopping by using ethylene diamine tetraacetic acid (EDTA) solution. Compared with the conventional protein argentation, the protein argentation provided by the invention has high sensitivity, short required time and high mass spectrum compatibility, and is suitable for the study of high-flux proteomics. The invention relates to the field of protein staining, in particular to the mass spectrum-compatible protein argentation on the electrophoretic gel.

The invention provides a chromosome immunoprecipitation method for DNA binding proteins in eukaryotic cells. The method comprises the following steps: crosslinking proteins and chromatins in the eukaryotic cells of eukaryotic cell suspension; pyrolyzing the eukaryotic cells; shearing a released crosslinked protein-chromatin complex; adding magnetic beads respectively coated with protein A, proteinG and protein L, or adding magnetic beads coated with protein A, protein G and protein L, and adding a desired antibody to the DNA-binding proteins; performing incubation; washing the magnetic beads;eluting the magnetic beads; performing decrosslinking; extracting and purifying DNA. The invention further provides a related kit. The chromosome immunoprecipitation method and kit for the DNA binding proteins in the eukaryotic cells can enrich a large number of target DNAs, and fragments can be well controlled between 100 and 500 bp, which is beneficial to improving the detection effect of scientific research and clinical samples and is favorable for the development of epigenetic research and clinical biomedicines and the detection of results. The method and kit are ingenious in design, operated easily and quickly, low in cost and suitable for large-scale popularization and application.

The present invention generally relates to treatment solutions for staining proteins including aluminum ions and staining methods using the same, and more specifically, to a treatment solution for staining proteins including aluminum ions, the treatment solution used when proteins are stained by a Commassie Brilliant Blue CBB stain, and a staining method using the same. The disclosed treatment solution including aluminum ions for CBB-staining proteins can decrease detection limits, thereby improving the sensitivity and reducing staining time as well as omitting a de-staining process.

The present invention generally relates to treatment solutions for staining proteins including aluminum ions and staining methods using the same, and more specifically, to a treatment solution for staining proteins including aluminum ions, the treatment solution used when proteins are stained by a Commassie Brilliant Blue CBB stain, and a staining method using the same. The disclosed treatment solution including aluminum ions for CBB-staining proteins can decrease detection limits, thereby improving the sensitivity and reducing staining time as well as omitting a de-staining process.

According to the present invention, the biological or pharmacological activity of a test material like a plant or herbal material, an extract of a plant or herbal material, a natural or synthetic compound or some combination thereof, can be quantified by observing the pattern of structural changes induced in a eukaryotic cell's proteins. These structural changes may be evidenced by protein phosphorylation, by protein-protein interactions and the like. The amount and nature of protein phosphorylation is qualitatively and quantitatively related to the in vitro concentration of biologically / pharmacologically active components to which the mammalian cells are exposed. Additionally, formation or loss of protein-protein complexes may be determined in whole cell homogenates through the use of non-denaturing electrophoresis and staining for proteins or protein phosphorylation. The present invention allows natural products to be formulated into nutritional supplements and pharmacological preparations of consistent biological / pharmacological activity without the need to identify any of the chemical constituents responsible for the biological or pharmacological response.

The invention discloses a rapid protein staining solution. The rapid protein staining solution is used for protein staining in SDS-PAGE. The rapid protein staining solution comprises, by mass volume,0.05%-0.1% of coomassie brilliant blue G250, 5%-15% of sulfate, 5%-20% of absolute ethyl alcohol, 1%-5% of polyethylene glycol and 1%-10% of an organic acid. The rapid protein staining solution is optimized on the basis of a traditional staining solution, a clearer protein strip is obtained, background staining is basically avoided, a decolorizing solution does not need to be used for decolorizingafter staining is finished, only tap water is used for washing, the detection time is greatly shortened, the efficiency is improved, no toxic or harmful reagent is used in the staining process, and harm to a human body is avoided.

The present invention discloses a high sensitivity and low background Coomassie brilliant blue (CBB) staining solution and a using method thereof, and belongs to the technical field of protein staining reagents of SDS-PAGE. The staining solution is composed of CBB R250 with a mass volume concentration of 0.03%-0.05%, acetic acid with a volume percentage of 2%-6%, ammonium sulfate with a mass volume concentration of 8%-15%, and ethanol with a volume percentage of 12%-16%. The CBB staining solution provided by the present invention is optimized on the basis of the traditional staining solution,and a clearer background is obtained, so that the detection sensitivity is greatly improved and the staining sensitivity reaches the nanogram level. According to the method for using the CBB stainingsolution provided by the present invention, without elution after staining, deionized water is used to wash to destroy the colloid state to complete decolorization, so that the detection time is greatly shortened, the method can be used for rapid protein staining of SDS-PAGE, and work efficiency can be improved.

Provided herein is a modified method of agglutination to detect infections caused by microorganisms including the steps of staining the test serum, plasma or blood or purified antibodies with a protein stain; mixing serum, plasma or blood with stained antibodies with an equal quantity of colored antigen particles on a glass slide; adding diluted Antiglobulin conjugated with Biotin to the mixture; subjecting the mixture to the step of mixing, adding diluted Avidin (preferably tagged with a visible indicator) to the mixture and thoroughly mixing all the ingredients.

The invention provides a mild laundry detergent and a preparation method thereof, and belongs to the technical field of daily chemical products. The mild laundry detergent provided by the invention isprepared from the following components in percentage by mass: 1 to 25 percent of amino acid surfactant, 1 to 25 percent of multi-element surfactant, 1 to 10 percent of preservative aid, 0.1 to 5 percent of non-phosphorus chelating agent, 0.1 to 1 percent of thickening agent and the balance of water. According to the invention, the amino acid surfactant contains amide groups, has strong interfacial force and strong adsorption capacity to protein stains, and can efficiently remove the protein stains in clothes. No preservative or protease is added into the raw materials of the mild laundry detergent provided by the invention, so that the mild laundry detergent is mild and small in irritation to a human body and does not easily cause allergy.

The invention is suitable for the technical field of daily cleaning products and provides a glasses cleaning agent and a preparation method thereof. The glasses cleaning agent comprises the followingcomponents in parts by weight: 1 to 2 percent of ampholytic surfactant, 0.12 to 0.2 percent of cationic surfactant, 0.5 to 1.5 percent of enzyme preparation, 0.01 to 0.02 percent of pH regulator and the balance of deionized water. In the glasses cleaning agent provided by the invention, various mild and non-irritant green raw materials are compounded to form a neutral enzyme-containing formula, the oral toxicity LD50 of the product is more than 5000 mg / kg of weight, the safety is high, protein stains and harmful flora attached to the surface of the glasses can be effectively removed, residuesof the cleaning agent is avoided, and the glasses cleaning agent is non-irritant to eye skin.

The invention relates to the technical field of intelligent washing machines and discloses a stain pretreatment system for a RFID-based domestic intelligent washing machine. The stain pretreatment system comprises a stain recognition system, an oily stain pretreatment system, an acid stain treatment system and a protein stain treatment system. The stain recognition system comprises a central processing unit, an input end of the central processing unit is in electrical connection with a stain recognition unit and a reader, and an input end of the reader is connected with an RFID tag through radio frequency signals. The stain recognition unit comprises an X-Spect scanner, and the oily stain pretreatment system comprises a first microprocessor. Stains on to-be-washed clothes are recognized through the stain recognition system and then pretreated by the oily stain pretreatment system, the acid stain treatment system and the protein stain treatment system, and suitable stain treating agentsare selected for treatment according to clothes information provided by the RFID tag.

The invention relates to the field of daily necessities, and in particular relates to a plant cleaning agent for cleaning human secretions. The plant cleaning agent comprises the following materials:isomeric octapolyoxyethylene polyoxypropylene ether, C9-11 fatty polyoxyethylene ether, cardanol polyoxyethylene ether, alkyl glycoside, coconut oil diethanolamide, sodium hydroxide, 1,3-butanediol, protease, essence, a nano silver solution, methylchloroisothiazolone, tetrasodium glutamate diacetate, citric acid and water. The plant cleaning agent provided by the invention has very strong emulsification decontamination ability, can penetrate into fibers more quickly, so that various stubborn stains on clothes can be more easily removed; and the protease can make protein stains such as blood stains, sweat stains, milk stains and oil stains easy to remove by washing, the nano silver solution can quickly kill various bacteria on women underwear, and the 1,3-butanediol has good moisturizing properties and can protect skin and do not hurt hands when added into a laundry liquid.

Provided is an oral composition that can sufficiently remove protein staining present on a tooth surface, can effectively prevent adhesion of protein staining on a tooth surface and can provide a good actual feeling of the cleaning effect in the oral cavity. The oral composition comprises the following components (A) and (B): (A) an N-acylamino acid or a salt thereof in an amount of 0.005 mass % or more and 0.3 mass % or less; and (B) pyrophosphoric acid or a salt thereof in an amount of 0.005 mass % or more and 0.5 mass % or less, in which a mass ratio ((B) / (A)) of the component (B) to the component (A) is 0.05 or more and 40 or less and a total content of the component (A) and the component (B) is 0.01 mass % or more and 0.6 mass % or less.

The invention discloses a fluorescent protein dyeing agent as well as a preparation method and application of the fluorescent protein dyeing agent. The structural formula of the fluorescent protein dyeing agent is as shown in the specification. A solution of the fluorescent protein dyeing agent has intense fluorescence, the dyeing agent comprises aldehyde groups which can be reacted with amino on protein to form covalent bonds for dyeing the protein, so that the dyeing agent has the advantages of being firm in dyeing, not liable to decolor, convenient to use, few in operation steps, short in time and high in sensitivity, is capable of improving the dyeing and fluorescence detection effect, and is beneficial to fluorescence or naked-eye recognition and detection.

The present invention discloses an environmental-protection laundry auxiliary agent for a movable laundry room, a preparation method and applications thereof, wherein the laundry auxiliary agent comprises the following raw materials by weight: 22-30 parts of fatty alcohol polyoxyethylene ether, 19-27 parts of polyacrylamide, 5-13 parts of glycerol monostearate, 1-8 parts of jojoba oil, 1-8 parts of nanometer titanium dioxide, and 8-16 parts of sodium acetate. The preparation method comprises: mixing polyacrylamide and deionized water to obtain a polyacrylamide solution; placing nanometer titanium dioxide into the polyacrylamide solution, and carrying out heating stirring to obtain a mixed material; and carrying out mixing heating stirring on fatty alcohol polyoxyethylene ether and a prepared sodium acetate alcohol solution, adding glycerol monostearate and the mixed material, carrying out heating stirring, adding jojoba oil, and carrying out microwave treatment and ultrasonic treatment so as to obtain the product. According to the present invention, the environmental-protection laundry auxiliary agent has advantages of strong detergency, quick effect, less odor and safe use, can perform permeation decomposition removal on various protein stains, human secretions, old blood stains and high temperature treated blood stains, and provides effective effects for washing and maintenance of linen.

A low dose of borax and of liquid detergent is delivered to wash water in a multi-chamber single dose unit containing borax in a powder chamber and a liquid detergent in a liquid chamber. The treatment removes proteinaceous stains without a proteinase enzyme.

The invention relates to the field of protein staining, in particular to a Commassie brilliant blue staining solution and a staining method and application of the Commassie brilliant blue staining solution in protein detection. The Commassie brilliant blue staining solution is an aqueous solution containing 10-20% (v / v) of alcohol, 4.5-10% (v / v) of acid, 5-10% (w / v) of ammonium sulfate and 0.01-0.1% (w / v) of Commassie brilliant blue. The staining method comprises the following steps: (A) staining: putting SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) gel in the Commassie brilliant blue staining solution and staining for more than 45min; and (B) destaining: flushing the stained SDS-PAGE gel with water till the staining solution is completely washed off. Through adoption of the Commassie brilliant blue staining solution and the staining method provided by the invention, under the condition that the sensitivity is not reduced, the SDS-PAGE gel is stained at low cost; moreover, the detained gel is hardly broken, thereby brining no trouble to the following mass spectrum research and the like.

The invention discloses a class of anti-tumor compounds containing a triazole heterocyclic structure and an application thereof, belonging to the technical field of medicinal chemistry. The compounds containing the triazole heterocyclic structure introduce quintuple 1,2,3-triazole ring in the form of side chains through the amino condensation, bromination, nitrine substitution, link reaction and other reactions on the basis of naphthalimide parent structure, select tetrazolium salt reduction to test 7721 hepatocellular in the experiment of inhibiting proliferation of tumor cells, and also select sulfonyl rhodamine B protein staining method to test MCF-7 breast cancer cells. The experiment results show that the anti-tumor compounds have wide anti-tumor activity and good inhibition effect on the normal growth of tumor cells derived from different tissues of liver cancer, breast cancer and the like.