The present invention discloses an environmental-protection laundry auxiliary agent for a movable laundry room, a preparation method and applications thereof, wherein the laundry auxiliary agent comprises the following raw materials by weight: 22-30 parts of fatty alcohol polyoxyethylene ether, 19-27 parts of polyacrylamide, 5-13 parts of glycerol monostearate, 1-8 parts of jojoba oil, 1-8 parts of nanometer titanium dioxide, and 8-16 parts of sodium acetate. The preparation method comprises: mixing polyacrylamide and deionized water to obtain a polyacrylamide solution; placing nanometer titanium dioxide into the polyacrylamide solution, and carrying out heating stirring to obtain a mixed material; and carrying out mixing heating stirring on fatty alcohol polyoxyethylene ether and a prepared sodium acetate alcohol solution, adding glycerol monostearate and the mixed material, carrying out heating stirring, adding jojoba oil, and carrying out microwave treatment and ultrasonic treatment so as to obtain the product. According to the present invention, the environmental-protection laundry auxiliary agent has advantages of strong detergency, quick effect, less odor and safe use, can perform permeation decomposition removal on various protein stains, human secretions, old blood stains and high temperature treated blood stains, and provides effective effects for washing and maintenance of linen.