The invention discloses nearly 480 novel
phosphorylation sites identified in
signal transduction proteins and pathways underlying
human Leukemia, and provides
phosphorylation site specific antibodies and heavy-
isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites / proteins, as well as methods of using the reagents for such purpose. Among the
phosphorylation sites identified are sites occurring in the following
protein types: adaptor /
scaffold proteins, acetyltransferases,
actin binding proteins, adhesion proteins,
apoptosis proteins,
calcium-binding proteins,
cell cycle regulation proteins,
cell surface proteins, channel proteins, chaperone proteins, contractile proteins,
cytokine proteins, cytoskeletal proteins,
G protein regulators and
GTPase activating proteins,
guanine nucleotide exchange factors,
helicase proteins,
immunoglobulin superfamily proteins, inhibitor proteins,
protein kinases,
lipid kinases, ligases,
lipid binding proteins, methytransferases, motor proteins, oxidoreductases, phosphotases, phosphodiesterases, phospholipases,
proteases,
receptor proteins, trascription factors, transferases, translation /
transporter proteins, and
ubiquitin conjugating
system proteins.