The invention discloses an affinity prediction and
analysis method for a human amphiphysin-1 Src homology-3 domain
binding peptide. The method comprises the following steps of: selecting an electrical property parameter, a three-dimensional property parameter, a hydrophobicity parameter and
hydrogen bond property parameter of an
amino acid, establishing physicochemical property classification scores of the
amino acid by adopting a
principal component analysis method, representing each
amino acid residue in
a peptide sequence by using the scores to realize the parameter-based description of
a peptide sequence structure, selecting characteristic parameters closely related to affinity from parameters of the
peptide sequence structure by using a
genetic algorithm, establishing a
relational model, namely an affinity prediction and analysis model between the characteristic parameters and the affinity by using a
Gaussian process, obtaining structural factors influencing the affinity of the
peptide by analyzing hyper-parameters of the model, and performing prediction by using the model to obtain an affinity value of the
peptide. By the method, a powerful tool can be provided for the high-
throughput screening of high-affinity human amphiphysin-1 Src homology-3 domain binding peptides, and development and researches on human amphiphysin-1 Src homology-3 domain
binding peptide inhibitors are further prompted.