84 results about "Treatment research" patented technology

The invention relates to a simulation and planning system for intraoperative radiation therapy and to a method allowing said system to be used for treatment studies, simulation, planning, training and recording, which system generally comprises a central processing unit or computer (1) for management and control and software-based communication with the rest of the devices and the user; one or several monitors or screens (2) for displaying images and peripherals responsible for gathering data relating to the actions performed by said user, a deformation simulation module for the virtual simulation of the deformation produced in the organs and tissues during the process; algorithms for instantly calculating the radiation dose applied during the radiation therapy treatment simulation and means for recording all the activities carried out and generating a full dosimetry report.

The invention discloses a method for rapid multi-nozzle 3D printing of a tumor tissue model using supporting bath, and relates to the field of biological 3D printing. The method mixes alginate, photocurable gelatin and the like to encapsulate cells and prints rapidly in the supporting bath, and printing of different cells is achieved by rapidly switching nozzles. By printing a normal tissue and atumor tissue in a complete model, the structure of a tumor tissue in vivo is better restored. The closed property of the hydrogel support bath provides a better sterile environment, and at the same time, uncontrolled cell density and species space due to settlement are avoided during printing. The rapid switching of the nozzles reduces the printing time to a certain extent, the decreasing of the activity of the cells is effectively slowed down in the printing process, and the integrity of the printing structure is effectively ensured. The problem of gel curing is effectively solved by printingwith a concentric needle. The tumor model is built faster and can be better used for tumor treatment research.

The invention provides an integrated intravascular OCT (optical coherence tomography) image and DSA (digital subtraction angiography) integrating offline treatment system, which comprises a data acquisition module, a DSA-image-based blood vessel segmentation and reconstruction module, an OCT-image-based blood vessel measurement module, a blood vessel three-dimensional imaging module, a DSA image and OCT image linkage module, an image reporting module and a transmission module. According to the system, a blood vessel is multidimensionally treated by combining a DSA image and an OCT image to further facilitate the accurate positioning of an interested blood vessel section; a blood vessel measurement result obtained by the DSA-image-based blood vessel segmentation and reconstruction module is compared with a blood vessel measurement result, obtained by the OCT-image-based blood vessel measurement module, on a cross section, so that the blood vessel treatment research accuracy of a doctor or a researcher after an operation is improved to facilitate the supply of a more perfect therapeutic schedule for an interventional operation in a similar case in the future.

The invention provides a construction method adopting PADs to implement cognitive tests, particularly a Matrics (Measurement and Treatment Research to Improve Cognition In Schizophrenia) consensus cognitive test battery PAD system construction method. The method includes the following steps: (A) a JOSN (JavaScript Object Notation) server-side interface for each test is established, so that a PAD side can obtain and update data; (B) at the PAD side, a test page is provided according to the requirement of each test, a testee is tested by the PAD side, so that test data are obtained; (C) a test result is analyzed and judged on the basis of the test data according to a test algorithm, so that a test conclusion is obtained and saved in a server side, and the test conclusion is presented at the PAD side; (D) test data and test conclusions are shared by different PAD sides and different departments. The PAD side / server side mode is used for substituting for the conventional paper and artificial test mode of the Matrics consensus cognitive test battery, and under the premise of keeping the original test process and specifications, test data are calculated and data are exchanged between the PAD side and the server side according to the uniform JSON interface.

The invention relates to the technical field of medicines, in particular to human recombinant epidermal growth factor (hrEGF)-modified cisplatin-loaded polymeric nanoparticles and a preparation method and application thereof. The hrEGF-modified cisplatin-loaded methoxy polyethylene glycol-polylactic-co-glycolic acid-polylysine (mPEG-PLGA-PLL) nanoparticles are obtained by preparing cisplatin-loaded nanoparticles by taking mPEG-PLGA-PLL as a carrier by an ultrasonic emulsification / solvent evaporation method, and then connecting an amino-terminated group and a hrEGF. The nanoparticles can be specifically combined with epidermal growth factor receptors (EGFR) of the surfaces of tumor cells, and the endocytosis is performed, so that the toxic or side effect of cisplatin is reduced, and the aims of reducing poisonousness and synergizing are fulfilled. The nanoparticles can be used for the preparation of anti-tumor medicines; and particularly, the treatment research of ovarian cancer proves that the nanoparticles have high targeting, have a strong killing effect, and have a good application prospect in the chemotherapy of ovarian cancer.

The invention discloses an artificial in-vitro preparation method of low molecular weight amyloid peptide oligomer and application, which is characterized in that the monomer of A beta polypeptide obtained with a gene recombination method is placed in a buffer system to react after dissolved by anhydrous dimethyl sulfoxide, thus naturally polymerizing to obtain oligomer. The method which obtains the monomer by gene recombination is economic and environmentally-friendly; when A beta serves as raw material to assemble amyloid peptide oligomer in vitro, a stable and even product is obtained by simulating in vivo physiological conditions and improving artificial cerebrospinal fluid components. The oligomer prepared with the method can serve as the standard reference for the detection and the therapy researches of various Alzheimer diseases, and can be applied in the diagnostic reagent and the therapy drug of the Alzheimer disease.

The invention relates to the effect of miR-451a cells in the non-small cell lung cancer. Migration of A549 cells can be significantly inhibited through overexpression of miR-451a in the A549 cells. By means of the bioinformatics analysis means, it is predicted that translation of the target gene mRNA is inhibited or the target gene mRNA is directly degraded by miR-451a through regional complementation with 3'-UTR of the target gene CAB39 mRNA; it is determined that the CAB39 gene is the target gene of miR-451a through double-luciferase reporter gene system analysis and Western Blot experiment verification. Finally, it is verified through the Transwell Assay and Flow Cytometry Assay experimental means that miR-451a performs the function of inhibiting migration of cells by reducing the CAB39 gene in the A549 cells, and a novel accurate treating researching and treating scheme is provided. By means of the effect, important research guidance and application value is provided for using miRNA for diagnosing and treating the non-small cell lung cancer and searching for drug targets in the fields such as tumor immunotherapy clinically.

The present invention discloses a system and a method for complex brain disease targeted combination treatment analysis. According to the system and the method, a modern data mining technology, a complex network analysis technology, a cloud computing technology and a large data technology are effectively combined; potential drug targets of diseases are dynamically queried, analyzed and computed according to different brain diseases; and problems in current medical research are solved, such as long data processing time, a long research period, lack of systematicness, a single research target and the like. According to the system and the method, an authoritative medical database abroad is combined and disease treatment method research is enabled to be systematic, processed and normalized. Besides, according to the system and the method, cost of medical disease treatment analysis and research is also reduced, and application of an advanced computer technology in the medical field is expanded. The present invention aims to establish brand-new network pharmacology study method and technology, is of special significance to breakthrough in brain disease prevention and treatment research, and takes positive efforts and does contribution for supporting and leading research, development and industrialization of innovative drug in China.

The invention provides an anti-tumor polypeptide nano-drug carrier targeting PD-L1 and application thereof. The anti-tumor polypeptide nano-drug carrier targeting PD-L1 can block a PD-L1 / PD-1 signal channel and activate the immune response of T cells, and a PD-L1 polypeptide nano-assembly comprises amphiphilic anti-tumor polypeptide, stearic acid coupled with a polypeptide side chain and enzyme responsive functional polypeptide. Specifically, the invention relates to a method for improving polypeptide stability through C18 modification and application thereof in activating immunotherapy. The nano-drug carrier is simple in preparation method, low in cost and high in practicability, shows a higher tumor inhibition effect compared with a single polypeptide, and is a tumor immunotherapy polypeptide nano-assembly with tumor site protease responsiveness, good biocompatibility, high stability and good stability. Important theoretical and clinical reference bases are provided for treatment research of various tumor immune checkpoints.

The invention discloses a feed additive capable of shortening the birth process of a sow and improving postpartum recovery. Each kg of feed additive contains the following components by weight: 2.5-7.5g of compound herbal leonuri, 7.5-12.5g of a compound micro-ecological preparation, 5-10g of a plant extract, 10-20g of animal oil and the balance of basic ration. A compound Chinese herbal medicine, the micro-ecological preparation and a plant extract are added to the ratio of the pregnant sow. The traditional Chinese medicine has the advantages of abundant resources, few drug residues and a small side effect; and the micro-ecological preparation has the functions of adjusting micro-ecological imbalance, keeping ecological balance and improving the organism immunity consistent with plant polysaccharide to achieve the effects of preventing and treating diseases. Prevention and treatment research is carried out on sow delivery and postpartum recovery by adopting pure traditional Chinese medicinal components, the micro-ecological preparation and the plant extract to provide a novel low-toxicity and low-residue feed additive for clinical production; and the inner conditions of a uterus are improved, the birth process of the sow is shortened and the postpartum recovery condition of the sow is restored.

The invention relates to a human anti-hepatitis B virus surface antibody as well as a preparation method and application thereof. A specific hepatitis B antibody with relatively high affinity activity on hepatitis B virus antigens can be obtained by virtue of antigen-antibody binding activity screening, wherein an amino acid sequence of a light chain variable region of the antibody is shown in SEQ ID No. 1 or SEQ ID No. 2, and an amino acid sequence of a heavy chain variable region of the antibody is shown in SEQ ID No. 3 or SEQ ID No. 4. According to the human anti-hepatitis B virus surface antibody disclosed by the invention, antibody mutation hot spots are analyzed by virtue of a protein molecule modeling technology and are subjected to saturation mutation by taking an amino acid as a unit so as to construct a mutant library and perform biological verification, and thus optimization of the antibody can be achieved. The human anti-hepatitis B virus surface antibody disclosed by the invention can be used for laying a foundation for preventing hepatitis B virus infection and performing treatment research at the same time, so that the human anti-hepatitis B virus surface antibody can be applied to the prevention and treatment of liver diseases caused by hepatitis B virus infection clinically.

The invention discloses a method for making a dry age-related macular degeneration disease model on a primate, namely applying sodium iodate onto the primate with a carotid artery injection method. The method is simple and easy, high in success rate and good in repeatability. The model made with the method is stable. The amount of sodium iodate needed is small, injury of large doses of sodium iodate to the whole body of the animal is avoided, and the animal can live for a long time so that follow-up tests can be made possible. A model of any one side or a model of double sides can be made as needed. When the one-side model is made, a lateral eye can not be hurt at all, and normal living vision of the animal is guaranteed. Comparison of two lateral eyes of one animal can be conducted to provide credibility for the test, and the number of animals needed for the test is reduced. The method provides a sound tool for the dry age-related macular degeneration research and has great significance for the pathological physiology research and treatment research of the disease.

The invention discloses a reconfiguration runoff production simulation method based on underlying surface features, and belongs to the technical field of hydrology, and the method comprises the following steps: S1, packaging runoff production calculation modes, and respectively numbering the runoff production calculation modes; S2, calculating to obtain a terrain index grid based on the basin DEMdata; S3, extracting to obtain vegetation grids and soil grids in the drainage basin; and S4, dividing the drainage basin into four areas of GX, DX, GC and FC based on the terrain index grid, the soilgrid and the vegetation grid, and respectively matching different runoff production modes in different areas to perform runoff production calculation. The accuracy and reliability of the calculationresult solve the problem of runoff calculation in mountainous small and medium-sized watersheds with obvious underlying surface feature spatial variation; a basin digital elevation model is mainly applied, rapid and automatic judgment suitable for different runoff production calculation method areas in a basin and direct calling of a runoff production calculation method based on underlying surfacecharacteristics are facilitated, and deep development of digital hydrology and mountain torrent prevention and treatment research of mountainous small and medium-sized basins is promoted.

The invention discloses a parallel intelligence-based chronic disease diagnosis and treatment system and diagnosis and treatment method. The system comprises an actual diagnosis and treatment system,a virtual diagnosis and treatment system and a virtual-real parallel execution system; the actual diagnosis and treatment system comprises an actual doctor, an actual patient and a process that the actual doctor diagnoses diseases for the actual patient and formulates an actual treatment scheme; the virtual diagnosis and treatment system comprises an intelligent doctor, an intelligent patient anda process that the intelligent doctor carries out intelligent diagnosis and makes an intelligent treatment scheme according to information of the intelligent patient. The virtual-real parallel execution system comprises interaction of disease diagnosis results and disease treatment schemes between the intelligent doctor and the actual doctor, and monitoring feedback and abnormal intervention of treatment effects in the actual patient treatment process. The chronic disease diagnosis and treatment system disclosed by the invention can ensure the accuracy and reliability of a diagnosis and treatment scheme, ensures the treatment effect of a chronic disease patient, and provides a reliable and effective solution for intelligent chronic disease diagnosis and treatment research.

The invention relates to the technical field of side slope stability prevention and treatment research and relates to a side slope rescuing counter pressure adverse construction method and a structure thereof. The side slope rescuing counter pressure adverse construction method comprises the following steps that 1, side slope body geometric elements are determined, and side slope body physical and mechanics parameters are determined; 2, counter pressure platform parameters are determined, and counter pressure materials are determined; and 3, an adverse method is adopted for carrying out construction. The side slope rescuing counter pressure adverse construction method has the beneficial effects of being convenient in construction, fast, safe, low in cost, small in disturbance to a slope body and the like. Moreover, the method can be applied to filling embankments of highways and railways, water conservancy embankment projects, soft soil foundation pit projects and various side slope rescuing projects.

The invention belongs to the technical field of radiation medicine, and specifically relates to a method for building a mouse acute radiation duodenitis model. According to the method, 8Gy gamma-ray irradiation is performed on mice, the irradiation part is the abdomen, duodenitis pathological analysis is performed on the mice after two weeks since the irradiation is conducted, and the protein expression levels of an apoptosis related factor BCL-2 and an inflammation related factor TGF-beta1 are detected. According to the invention, an irradiation method which is closer to clinical radiotherapyis adopted to build the radiation duodenitis model, thereby providing an experimental basis for prevention and treatment research for clinical radiation intestinal injuries.

The invention discloses coptis chinensis skin healing paste and a clinical research method for treating burns by using gauze strips of the paste, and particularly relates to the field of burn treatment research. The coptis chinensis skin healing paste is composed of coptis chinensis, angelica sinensis, phellodendron chinense schneid, radix rehmanniae recen, rhizoma wenyujin consium, raw sanguisorba officinalis, frankincense, myrrh, raw rhubarb, beeswax and sesame oil. The coptis chinensis skin healing paste is soaked onto multiple layers of gauze strips to obtain coptis chinensis skin healingpaste gauze strips. The specific research method comprises the following steps: firstly, setting a treatment group and a control group for synchronous treatment; after treatment for 7d, carrying out wound surface bacterium culture of a the patient, and comparing and analyzing two groups of multiple data; finally, a statistical method is adopted for processing, and a result is analyzed. The optimized dosage form of the coptis chinensis skin healing paste is made into an emulsion gauze strip, so that the coptis chinensis skin healing paste has good effects of clearing away heat and toxic materials, promoting tissue regeneration and healing skin, moistening dryness and relieving pain, can effectively reduce volatilization of medicines, protect wounds and relieve pain during dressing change, is uniform in medicine efficacy, and plays a role in smooth drainage.

The invention provides a pH-responsive nanoparticle self-assembled aggregate molecular probe, and a preparation method and application thereof, and belongs to the technical field of nanoparticle self-assembly. The surfaces of nanoparticles are modified with a substance containing a pH response group, self-assembly of light-triggered nanoparticles is achieved, and the obtained pH response nanoparticle self-assembled aggregate absorbs wavelength red shift, has a photo-thermal treatment effect, and is successfully applied to photoacoustic imaging and photo-thermal treatment research of living tumors, meanwhile, the nano-molecular probe is successfully prepared by utilizing radionuclide labeling, and the nano-molecular probe has important guiding significance on metal nano-materials in clinical tumor diagnosis and treatment research.

The invention provides a preparation method for a composite photothermal material. The method comprises the following steps: adding potassium hexachloroplatinate solution to G4.5-COONa solution (pH=2), regulating a pH value to be 9.16, after reacting for 12 h in a dark place, adding sodium borohydride solution, after the reaction, dialyzing in a dialysis bag of which a cut-off molecular weight is3500 Da, to obtain the composite photothermal material (DEPt-COOH). A polyamide-amine tree-form macromolecule of which an end group is a carboxyl is used as a carrier, and metal nano photothermal particles are coated in the interior to synthesize a novel compound (DEPt-COOH) which is used for a therapy research of bone tumor. In the multi-functional compound, a poly carboxyl terminal is efficiently chelated with the surface of a corrosion bone so as to realize efficient targeted delivery of the composite material in a bone tumor focal area. Efficient killing to the bone tumor is realized through photothermal therapy, and side effects to a normal tissue are greatly reduced.

The invention provides an application of a tumor hypoxic microenvironment targeting traditional Chinese medicinal small-molecular composition in tumor treatment. Tumor hypoxic microenvironment targeting traditional Chinese medicinal small molecules are screened by taking a tumor hypoxia-inducible factor 1[alpha] and a downstream VEGF (vascular endothelial growth factor) thereof as targets, and the traditional Chinese medicinal small molecules are combined into the composition for conducting tumor prevention and tumor treatment researches; based upon screening, it discovers that berberine, cinnamaldehyde, gingerol, evodiamine and notoginsenoside are relatively good in effects on regulating the tumor hypoxia-inducible factor 1[alpha] and the VEGF, and it further proves that the composition of the berberine, the cinnamaldehyde, the gingerol, the evodiamine and the notoginsenoside at the ratio of 8 to 1 to 1 to 1 to 1 to 1 achieves an optimum effect on regulating the hypoxia-inducible factor 1[alpha] and the VEGF and the composition has a good tumor prevention and treatment effect.

The invention relates to the field of animal models and particularly discloses an establishment method for a model for promoting tongue cancer development and lymphatic metastasis of mammals. According to the establishment method for the model for promoting tongue cancer development and lymphatic metastasis of the mammals, the mammals are fed with a 4-NQO solution and a NaNO2 solution in sequence for a period of time; the mammals are subjected to normal observation every week and killed randomly for HE staining observation and immunohistochemistry CK8 staining observation; when white patches are generated on the tongue faces of the mammals, invasive carcinoma is found through HE staining observation, and lymphatic metastasis is generated through CK8 staining. The establishment method for the animal model for promoting tongue cancer development and lymphatic metastasis with sodium nitrite is established, the progress of the actually-generated tongue cancer is simulated, and a novel and powerful tool is provided for research on the influences of sodium nitrite on the tongue cancer, treatment research on the tongue cancer and the like.

The embodiment of the invention discloses a method for simulating the tumor cell state through a Boolean network. The method comprises the steps that continuous expression spectrum data of a biological sample gene is acquired through a gene chip collecting technique, and an initial gene regulatory network is constructed according to the continuous expression spectrum data; deletion regulation is conducted on the initial gene regulatory network on the basis of the preset gene relevance to obtain a related gene regulatory network, and the related gene regulatory network is calculated through a Boolean function to obtain a single attractor of the related gene regulatory network; the regulatory range of a target gene in the initial gene regulatory network is determined and converted into an attractor of a compound Boolean function according to the single attractor of the related gene regulatory network, and then the converted attractor and a cell attractor are matched to determine the tumor cell state. According to the method, an overall understanding on the tumor gene can be achieved, tumors are quickly identified, and a powerful theoretical framework is provided for tumor treatment research.

The invention relates to a pig SOX10 mutant gene causing inner ear hypoplasia and an application of the pig SOX10 mutant gene. The pig SOX10 mutant gene is characterized in that the 321st basic groupcytosine C of an SOX10 gene coding region is subjected to replication (SOX10 c.321 dupC), the mutation can cause infertility of the inner ear and is autosomal dominant inheritance, and can be used forpathogenesis, prevention, diagnosis and treatment research of infertility of the inner ear of a human body. The pig SOX10 mutant gene can be used for preparation of a disease animal model with incomplete development of the inner ear. The model can be used for research on pathogenesis, prevention, diagnosis and treatment on human inner ear hypoplasia, and is of great significance to research on inner ear hypoplasia.

The invention relates to the technical field of traditional Chinese veterinary medicines, in particular to a construction method for a chicken construction-aspect pattern model. The construction method for the construction-aspect pattern model comprises the steps that SPF chickens of 30-60 days old are taken as experimental animals, and infection is carried out by adopting intramuscular injectionof avian escherichia coli virulent strains in chests. The construction method for the chicken construction-aspect pattern model creatively adopts a mode of intramuscular injection of the avian escherichia coli virulent strains and has the advantages of being capable of rapidly transmitting the pathogenic factors into the construction aspect, simple in construction, easy in operation, obvious in clinical symptoms, stable, reliable and suitable for traditional Chinese medicine prevention and treatment research on chicken yingfen patterns of traditional Chinese veterinary medicines.

The invention discloses a VSX2 green fluorescence reporter gene human induced pluripotent stem cell line and a construction method thereof. The constructed VSX2 green fluorescence reporter gene hiPSCline is bright in reporter fluorescence expression, high in specificity and capable of being stably expressed for a long time, and strong fluorescence signals can still be detected during tissue fixation and end detection of frozen sections. According to the VSX2 green fluorescence reporter gene hiPSC line and the construction method thereof, a useful tool can be provided for retina related disease modeling, development mechanism and prevention and treatment research, and the VSX2 green fluorescence reporter gene hiPSC line is used for tracking of retinal cell fate and return, optimization ofa differentiation system, separation and purification of eye cups, even drug screening, cell transplantation and other downstream research.

The invention relates to a Chinese herbal medicine recommendation system based on deep learning. The method comprises the following steps: by taking a public 'typhoid theory' data set which is widely accepted in the field of traditional Chinese medicine as a research object, establishing a standardized database through 'Multi-hot' code preprocessing on symptoms and traditional Chinese medicine prescription information; simulating an internal matching rule between a clinical symptom group and Chinese herbal medicines by using a convolutional neural network, designing a clinical traditional Chinese medicine prescription recommendation algorithm by using a Pytorch framework, and comparing with three traditional machine learning models including a support vector machine, a Bayesian classifier and logistic regression; therefore, the effectiveness and application value of the algorithm are explained from two dimensions of quantification and qualification. According to the method, Chinese herbal medicine prescriptions can be accurately recommended, and the method plays an important role in promoting traditional Chinese medicine diagnosis and treatment research based on artificial intelligence.

The invention discloses a method for constructing a liver and immune double-humanized large animal model by utilizing single stem cell transplantation. The method comprises the following steps: acquiring human stem cells, transplanting a single stem cell into a large animal with liver injury, embedding human liver cells, forming a humanized immune system, and the like. The method can be used for researching the pathogenesis of human body diseases and researching, developing and screening new clinical medicines, and also can be used for obtaining an infectious disease model, a liver disease and drug metabolism model, a tumor growth and cancer immune model, an organ transplantation model, an autogenous immune disease model and the like which more accord with the development history of human diseases; and the humanized model which is convenient, simple, easy to obtain and high in similarity with a human body is provided for clinical liver disease treatment research.

The invention provides application of NKG2A in detection of human NK cells in a mouse humanized tumor model, and belongs to the technical field of biology. The invention relates to an application of NKG2A in detection of human NK cells in a mouse humanized tumor model. The NKG2A fragment is adopted as a detection target, human NK cells and human tumor cells can be distinguished in a mouse tumor-bearing tumor model, and the NKG2A fragment has the characteristics of short detection time, high detection efficiency and high detection sensitivity, is beneficial to research on non-clinical pharmacokinetics, particularly animal experiments, exploration of in-vivo dynamic and prediction of a treatment scheme in the cell treatment research and development process, and has a wide application prospect. The method is very helpful for reducing the uncertainty of cell therapy, increasing the curative effect and reducing the toxic and side effects, and has important clinical guiding significance.

The invention relates to the field of a medical technology. In recent years, the beta2 adrenocepter (ADRB2) and its signal pathway have been given more and more attention in the cancer prevention and treatment research field. The invention provides an application of the ADRB2 inhibitor in the preparation of a medicine for treating liver cancer. Especially, the ADRB2 inhibitor and sorafenib can be combined to effectively inhibit growth of liver cancer and effectively inhibit efficiency of tumor forming by transplantation of liver cancer so as to reduce evolution of liver cancer. The application is easy for clinical application and popularization.

The invention discloses a tupaia belangeri MHC-DRB1*1101 gene sequence and application thereof. A nucleotide gene sequence is as shown in SEQ ID NO.1. The tupaia belangeri MHC-DRB1*1101 gene sequence is applied to establishment of HCV (hepatitis C virus) models, and efficiency in establishment of tupaia belangeri HCV models is greatly improved while loss of resources is reduced. By combination with successfully established tupaia belangeri HCV models, a new target can be possibly found for HCV treatment to provide most basic information for further treatment researches of tupaia belangeri HCV models, and a start is provided for other tupaia belangeri allele gene researches.