152 results about "Large cell" patented technology

An expandable stent has larger cells located at the distal end of the stent than in the body portion so that a catheter balloon can more easily protrude into the cells to increase stent retention relative to the balloon. The intravascular stent has a plurality of cylindrical rings connected by links, the spacing of which is a factor in defining the cell size. The stent can be compressed or crimped onto a balloon catheter to a very low profile and maintain a high degree of stent retention due to increased spacing between rings in the region of the distal end ring.

Methods and reagents are provided for determining the activation state of a signal transduction pathway signaling protein. There exists a need in the art for methods that can monitor the efficacy of a signal transduction inhibitor in a patient. Other needs exist for detecting and monitoring certain disease or disorders that are associated with aberrant activation of a signal transduction pathway signaling protein. The present assay provides a highly sensitive assay that is also useful in patient populations in which obtaining a large cellular sample is difficult, for example, neonates.

A method and apparatus is provided for transmitting an orthogonal frequency domain multiple access (OFDMA) signal including a synchronization channel signal transmitted within a localized portion of a bandwidth of the OFDMA signal (818), the synchronization channel signal having predetermined time domain symmetry within the localized portion of the bandwidth (816) and including information for providing at least partial cell identification information (812). The synchronization channel signal enables an initial acquisition and cell search method with low computational load which provides OFDMA symbol timing detection and frequency error detection (1112) and frame boundary detection and cell specific information detection (1114) in an OFDMA system supporting multiple system bandwidths, both synchronized and un-synchronized systems, a large cell index and an OFDMA symbol structure with both short and long cyclic prefix length.

Disclosed is a hierarchical random access method for a wireless communication system having a significantly large cell. According to the present invention, a length of a preamble sequence and a length of a reference slot may be designed based on a terminal having greatest capacity of adjusting a timing error arrived at a base station, and a slot length may be designed to be an integer multiple of the length of the reference slot depending on a timing error correction capacity, thereby enabling terminals to use various slot lengths.

In a liquid crystal display device, a thickness of a liquid crystal layer in each pixel is adjusted based on a display color (red, green, and blue). For example, thicknesses of color filters corresponding to the display colors are varied based on the display color to set the thickness (cell gap) of the liquid crystal layer in each pixel to a predetermined value corresponding to a transmittance of the liquid crystal. A spacer provided to maintain the cell gap at a constant value is not provided in a region, among the plurality of display colors, having a large cell gap and the spacer is provided above the color filter or the like at a pixel region having smaller cell gap. With this structure, all spacers provided within a cell can substantially contribute to maintaining the gap.

Provided are methods for producing and screening proliferated populations of CD34-negative progenitor cells, mucosal mast cells, connective tissue-type mast cells and basophil cells. The methods generate uniform proliferated populations of cells. The proliferated populations contain a uniform population of a size suitable for use in high throughput screening methods, for example, screening for agents that alter exocytosis. The invention includes screening the proliferated populations with at least one candidate bioactive agent, and evaluating the cells to detect a cell with an altered phenotype. The invention also includes isolating a candidate bioactive agent that causes the altered phenotype. Additionally, cells formed according to the described methods are also encompassed by the invention.

The invention provides a terahertz metamaterial device for detecting circulating tumor cells and a preparation method thereof. The device is mainly characterized with a stereo terahertz metamaterial,supplemented by a liquid diversion region. Only a small amount of blood sample is introduced from an inlet hole in a cover plate, and then blood cells of smaller size can be filtered according to structural features. Cancer cells of larger size are fixed at the sensitive positions, namely the clamping positions, of a metamaterial resonant structure. The cancer cells in the sample can be detected by comparing spectral changes before and after the sample is introduced. In addition, the device enables single separation of cancer cells, and can be used for subsequent biological study of individualcancer cells, so as to master the trait changes of each individual cancer cell stimulated by external conditions based on spectral analysis. Compared with the prior art, the device has high sensing sensitivity, and has remarkable effects when being used for fixed-point capture or large cell monitoring.

A method and apparatus for cell harvest of production scale quantities of cell cultures using single use components comprising a flexible membrane mounted on a rigid frame and is supported within a multiple use rigid centrifuge bowl, such single use components including a core with an increased diameter and an internal truncated cone shape in order to permit the system to maintain a sufficiently high angular velocity to create a settling velocity suited to efficiently processing highly concentrated cell culture streams. Features which minimize feed turbidity, and others which permit the continuous or semi-continuous discharge of cell concentrate, increase the overall production rate over the rate which can be achieved using current intermittent processing methods for large cell culture volumes. Injection of a diluent during the cell concentrate removal process permits more complete removal of viscous cell concentrates.

The invention aims to provide an efficient culture method of CIK cells. The prepared CIK cell has the characteristics of high multiplication speed, large cell quantity, high cell viability, high killing capacity on cancer cells and the like. The efficient culture method mainly characterized in that a culture flask coated with laminin and fibronectin is used for culturing PBMCs (peripheral blood mononuclear cells), and the cells can be in a half-adherence state by means of the absorption effect of the two kinds of protein, accordingly, the cells can be stimulated by various factors, and the two kinds of protein can promote CIK cell multiplication; and with the adoption of an anti-CD28 monoclonal antibody, the co-stimulation signal of a T cell subset in the CIK cells can be stimulated, and the activation of the CIK cells is promoted. So far, a research report on jointly using laminin, fibronectin and the anti-CD28 monoclonal antibody to prepare the CIK cells is absent. The method for increasing the cell number and improving the killing activity by adding the three factors during preparation of the CIK cells is firstly provided.

A method for performing a cell reselection in a system comprised of a hierarchy of cells, wherein cells of one layer of the hierarchy have a size that differs from cells of another layer of the hierarchy. The method includes steps of (a) identifying to a user equipment a layer to which individual cells in a neighbor cell list are associated; (b) when performing neighbor cell measurements for reselection purposes, avoiding a measurement of cells in the list that are larger than a current serving cell unless a Cell Selection (S) parameter falls below a search threshold parameter (Ssearch), and is greater than zero; and (c), if S is less than or equal to zero, and no cell reselection to a better cell is in process, beginning a measurement of neighbor cells without regard for their hierarchical level. If the user equipment has made cell reselection to N different cells on the same hierarchical layer within a time Tmax, the user equipment initiates a reselection procedure for larger neighbor cells. This procedure accommodates user equipment that is moving at a relatively high speed through the cells. If the user equipment locates a larger cell, which fulfills cell reselection criteria Sn > 0 and Qn > Qs+Qoffsets,n+Qhyst for a period of time Treselection, cell reselection to the larger cell is made. Furthermore, if the user equipment performs a cell reselection to the larger cell, the user equipment does not attempt to reselect to a smaller cell within a time X, unless an immediate cell evaluation is triggered, wherein the time X can be pre-specified, or can be set by the network.

The invention discloses a cervical cell pathological slice image pathological cell identification method and system based on cell mass, which comprises the following steps: extracting a single cell mass region on the cervical pathological slice image; extracting the single cell mass region on the cervical pathological slice image; counting the size of the cell mass region, and treating the cell mass whose size exceeds a predetermined threshold value as a super-large cell mass; the superlarge cell mass being divided into daughter cell mass; the region to be recognized being obtained by boundaryfilling treatment of the cell cluster region other than the super-large cell cluster and the daughter cell cluster obtained by splitting; a lesion cell being identified in that region to be identified. The invention aims at the whole slice image of the massive pixels, takes the cell cluster as the processing and recognition unit, instead of the conventional image partition and fusion frame, whichis more suitable for the characteristics of the cervical cell pathological slice image, and improves the efficiency and the precision at the same time.

Techniques for organizing a cell library permit a large number of cells. To improve design accuracy using cell libraries, very large cell libraries are needed. However, optimization tools are not able to use very large cell libraries directly, since their results suffer. Very large cell libraries are organized into sublibraries that are adapted to be processed by optimization tools. This allows improvement in the design quality of integrated circuits, while allowing the designs to be processed by optimization tools.

The invention discloses a method of producing a foot-and-mouth disease vaccine by using a large-scale high-density BHK-21 cell adherent culture technology. The method comprises the following steps of: 1) thawing and primarily amplifying cells; 2) conducting large-scale and high-density cell culture in a medium-volume bioreactor, and after the culture is completed, diluting the high-density cell sap in a large cell culture pot; and 3) inoculating foot-and-mouth disease seed virus, harvesting all virus suspension under proper conditions, and preparing the foot-and-mouth disease vaccine through the following steps. The process method is free from the process of acclimating the adherent cells into suspension cells; the necessarily gradual amplification process in the existing technology of preparing the foot-and-mouth disease vaccine by conducting large-scale suspension culture of the BHK-21 cell can be avoided, the technology flow can be obviously simplified, the production cycle can be shortened, the escape of the foot-and-mouth disease virus can be furthest reduced, and the pollution possibility can be decreased.