Method of preparing foot-and-mouth disease vaccine by using BHK-21 adherent cells

Abstract

The invention discloses a method of producing a foot-and-mouth disease vaccine by using a large-scale high-density BHK-21 cell adherent culture technology. The method comprises the following steps of: 1) thawing and primarily amplifying cells; 2) conducting large-scale and high-density cell culture in a medium-volume bioreactor, and after the culture is completed, diluting the high-density cell sap in a large cell culture pot; and 3) inoculating foot-and-mouth disease seed virus, harvesting all virus suspension under proper conditions, and preparing the foot-and-mouth disease vaccine through the following steps. The process method is free from the process of acclimating the adherent cells into suspension cells; the necessarily gradual amplification process in the existing technology of preparing the foot-and-mouth disease vaccine by conducting large-scale suspension culture of the BHK-21 cell can be avoided, the technology flow can be obviously simplified, the production cycle can be shortened, the escape of the foot-and-mouth disease virus can be furthest reduced, and the pollution possibility can be decreased.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and is specifically described as a method for preparing a foot-and-mouth disease vaccine by using BHK-21 cells in a bioreactor through one-step high-density large-scale adherent culture. Background technique [0002] Foot and mouth disease (FMD) is an acute, febrile, highly contagious infectious disease transmitted by artiodactyls caused by foot and mouth disease virus (FMDV), with more than 70 susceptible animals. Foot-and-mouth disease is highly contagious and can cause a decline in animal production performance. Once an outbreak occurs, it will cause huge economic losses to the animal husbandry in the infected area and seriously affect international trade. It is listed by the World Organization for Animal Health as an epidemic that must be reported as soon as possible. one of the diseases. [0003] FMDV belongs to the genus Aphthovirus of the family Picornaviridae, and has many serotypes, in...

AI Technical Summary

Problems solved by technology

These 4-5 step-by-step scale-up steps need to consume a large amount of medium, which increases the difficulty of cultivation and the possibility of contamination. Each step of scale-up needs to cultivate cells for 3-4 days, so the period of the scale-up process is 15-20 days. , the whole production process cycle is 30-40 days