The invention discloses a full-automatic cell viability analysis system which comprises the following steps: adding a trypan blue dye on a cell slide, inserting the slide into the whole system, triggering the whole system, calculating the cell concentration and the survival rate through an algorithm after imaging, withdrawing the slide from the device, completing the imaging process, and insertingthe slide into a system device, wherein the system device comprises a flat panel display screen, an operation keyboard, supporting legs, a slot, a box body, a control main board, a fixed rod, a CCD imager, a microscope barrel, a supporting rod, a fixing block, an anti-slip pad, a supporting plate, a connecting rod, a sliding groove, a sliding block, a supporting frame and a toothed belt. The trypan blue dye is added to cells on the cell slide, CCD imaging is controlled, five visual fields are photographed at one time, data is transmitted to a computer, cell concentration, survival rate and the like are calculated through the algorithm, intelligent imaging is achieved, machine processing is fast, errors are not easy to occur, full automation is achieved, a result can be directly obtained as long as the slide is inserted, and data acquisition and cell viability analysis can be directly and conveniently executed.