46 results about "First polar body" patented technology

The invention relates to a method for producing viable tetraploid bivalve molluscs, by fertilisation of diploid female ovocytes with diploid male sperm, followed by the induction of the retention of the first polar body of the fertilised eggs, isolation from the obtained larvae of a larval sub-population enriched in tetraploids and selective raising of said sub-population.

The invention discloses a preparation method of young mollusks of Hongkong tetraploid oysters. The method comprises the following technical steps of selectively choosing triploid eggs, carefully choosing diploid sperms, inhibiting second polar bodies of oosperms, obtaining tetraploid young mollusks, and raising tetraploid progenies. The feature of normal reduction mitosis of some triploid eggs is adopted so as to create a raising method for preparing tetraploid oysters by inhibiting second polar bodies of oosperms (triploid eggs +diploid sperms). The method differs from a method for obtaining tetraploid oysters by inhibiting first polar bodies of oosperms. The preparation method makes a great difference in a traditional preparation mode and helps break the monopolies of the US, France, Korea and other foreign countries on raisins technology of tetraploid oysters. It is the first time to obtain livable young mollusks of Hongkong tetraploid oysters. The method makes the biological method of producing 100% Hongkong triploid oysters feasible, promote the steady development of the industry of Hongkong oysters in the coastal region of southern China and offers the possibility of achieving improved varieties of the industry. The preparation method of young mollusks of Hongkong tetraploid oysters is being advantaged by being implementable, reusable, and applicable.

The invention discloses a time point quantification treatment method for producing a Crassostrea hongkongensis all-triploid. The time point quantification treatment method in which a fertilized egg development stage is taken as a biological indicator is created through the technical links of collection of single fertilized eggs, timely treatment of the fertilized eggs, detection of larva ploidy and the like, so that a 100-percent Crassostrea hongkongensis all-triploid can be obtained. Through optimization and pairing of the single fertilized eggs, the defect of poor mixed fertilized egg synchronicity in a conventional Crassostrea hongkongensis triploid induction process is overcome effectively, and zygotes with high synchronicity are obtained. A conventional fixed thinking mode in which a time point when 30-50 percent of a first polar body occurs is taken as a treatment time point and the optimal time interval is 20 minutes is overturned. Instead, the fertilized egg development stage is taken as the biological indicator, and A+1 / 3B and B+C are taken as a treatment time point and a treatment time interval respectively, so that the influences of external environmental conditions such as temperature on treatment are avoided, and the Crassostrea hongkongensis all-triploid can be induced successfully.

The invention discloses an induction method for a haliotis discus hanai triploid, and relates to a shellfish culture method. The method comprises the steps of selecting abalone seeds and promoting maturity; performing artificial spawning induction; selecting and processing gametes; performing synchronous fertilization; performing medicine induction; removing medicine; performing incubation. On thepremise of ensuring haliotis discus hanai seed gonad maturity and synchronous fertilization, the induction condition is further optimized, and therefore the haliotis discus hanai triploid is obtainedefficiently and stably. Synchronous fertilization is ensured, the phenomenon that gametes are in contact in advance in the discharge process is strictly avoided, microscopic examinations determine that no accident fertilization exists, and the consistency of synchronous fertilization and germ cell development is ensured. The induction conditions are optimized, wherein constant microscopic examination observation determines that 70-80% of contrast group germ cells PB1 are adopted as a processing time node, it is ensured that a first polar body is effectively released, and tetraploids and aneuploids are avoided. According to the stable and efficient induction rate, the induction rate of the triploid is 100% or is close to 100%.