The invention discloses a Ni-Co-S loaded porous carbon material based on yeast cells. The preparation method comprises the following steps: preparing carbonized yeast cell porous carbon, carrying outhydrothermal reaction on the carbonized yeast cell porous carbon, cobalt nitrate, nickel nitrate and urea, and oxidizing and vulcanizing to obtain the material, the obtained material is of a core-shell structure, and cobalt and nickel elements are uniformly loaded on the surface of the yeast cell porous carbon in the form of NiCo2S4; wherein the shell-core structure is formed by carbonizing ellipsoidal single-cell fungal yeast, and carbonized yeast cells are of a hollow carbon sphere structure. The preparation method comprises the following steps: 1) preparing yeast cell porous carbon; 2) adsorbing cobalt-nickel metal ions by yeast cell porous carbon; and 3) vulcanizing the yeast cell porous carbon adsorbing the cobalt-nickel metal ions. When the material is used as a supercapacitor, the material is charged and discharged in a range of-0.2 to 0.5, and when the discharge current density is 1 A / g, the specific capacitance is 700 to 800 F / g. The invention has the following advantages: noharm is caused to human body; and the tolerance problem of the yeast cell metal salt solution is solved.