32 results about "Immature oocyte" patented technology

An assembly is disclosed that is to be used for the culturing of specimens such as embryos and gametes for use in in vitro fertilization. The assembly includes an annular ring which is affixed to the stage of an optical viewing instrument, such as a microscope. The viewing instrument is focused on a point which lies inside of the ring at a predetermined distance from the center of the ring. Circular specimen dishes having a plurality of specimen wells in which the specimens in question are cultured or grown is removably positioned inside of the ring. The specimen wells have bottom walls which are configured so as to ensure that the specimens in the wells will gravitate to the same predetermined position in each of the wells. That position coincides with the focus point of the viewing instrument. When the specimen culturing dishes are placed inside of the ring, they can be rotated inside of the ring to bring the specimens in each well sequentially into the focus point of the viewing instrument whereby the specimens in each dish can be quickly and accurately monitored for growth and development. Other devices are disclosed which enable the rotation of the dish and proper alignment of the specimens in each well with the focus point of the viewing instrument. The assembly can be used for a wide range of specimen culturing. Typical specimens include animal and human cells, tissues, stem cells, embryos, oocytes, immature oocytes, sperm precursor cells, embryonic cells, blastocysts, and spermatozoa.

The invention discloses mature optimized human oocyte cytoplasm fluid. The invention develops the mature optimized human oocyte cytoplasm fluid by adding some important hormones, energy substances and efficient antioxidant melatonin on the basis of low-sugar TCM199 tissue culture solution and patient autoserum. The invention develops a culture solution which is suitable for mature optimization of the oocyte cytoplasm in IVF-ET period and also is suitable for reutilization after in vitro maturity of the immature oocyte in the period. The mature optimized human oocyte cytoplasm fluid is capable of improving the maturity degree of the cytoplasm of the matured oocyte. A clinical result proves that the high-quality embryo rate of the oocyte is greatly increased, the development potential of the immature oocyte is increased and some immature oocytes in the IVF-ET period have a reutilization value.

The invention relates to the technical field of artificial reproduction of fish, in particular to a method for improving the efficiency of artificial reproduction of long thin loach. The specific steps are as follows. Firstly, the broodstock are carefully screened and temporarily kept in polyvinyl chloride cages, and then the broodstock are classified. According to different developmental levels, different oxytocin needle intervals and drug dosages are selected, which is beneficial to the protection of the broodstock. With the synchronous maturation of oocytes, the proportion of immature eggs in the induced oocytes decreases from 10% to 3%, and then the induced oocytes are treated to mix the sperm and eggs evenly, and finally the broodstock is nursed to minimize the number of spawning broodstock. The injury and the death of postpartum broodstock have protected the limited long thin loach resources. The mortality rate of postpartum broodstock in traditional methods is as high as 70% to 100%, and the mortality rate is 10% under the technical conditions.

The invention discloses an in-vitro maturation culture method for an oocyte mouse and a method for establishing a parthenogenetic embryonic stem cell line. In the in-vitro maturation culture method, an immature oocyte is cultured in a basic culture solution in which the HCG (human chorionic gonadotropin) and PMSG (pregnant mare serum gonadotropin) are added, and the maturation rate of the oocyte can reach more than 83 percent. In the method for establishing a parthenogenetic embryonic stem cell line, the parthenogenetic activation culture development is carried out on the mouse oocyte after in-vitro maturation culture to obtain the embryonic stem cell line; a hepatocyte obtained by the method has no immunogenicity, is simultaneously equivalent to a stem cell derived from a fertilized embryo on totipotency and has guiding significance for utilizing in-vitro maturation of the immature oocyte of a human and separating parthenogenetic embryonic stem cells. Aiming at the current conditions of human ovum donation shortage, great discarding of clinical immature oocytes by an assisted reproductive technology, and the like, the two methods are combined to lay the foundation for developing and utilizing the in-vitro maturation culture of the immature oocyte of people and further researching the parthenogenetic embryo by utilizing an in-vitro mature ovum and separating the embryonic stem cells.

The present invention relates to cell culture, and specifically discloses a culture scheme for immature oocytes, which can promote the in vitro maturation of immature oocytes, specifically comprising follicular granulosa cells and a culture medium for cultivating the follicular granulosa cells, said culture The culture solution of follicular granulosa cells contains CNP or its variant or its analogue and HDAC (histone deacetylase) inhibitor. The present invention further provides an in vitro maturation culture solution comprising CNP or its variants or analogs thereof and HDAC inhibitors and related compositions thereof, as well as the above-mentioned medium, culture solution and composition in promoting the in vitro maturation of immature oocytes Applications.