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In-vitro culture solution of immature oocytes, and preparation method and application of in-vitro culture solution

An in vitro culture, oocyte technology, applied in the field of assisted reproduction, to restore meiosis, improve the rate of high-quality embryos, and improve immunity

Active Publication Date: 2020-08-11
成都艾伟孚生物科技有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

However, a technical solution that can make the nucleus and cytoplasm of immature oocytes mature synchronously, and improve the fertilization rate and high-quality embryo rate has not yet been proposed.

Method used

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  • In-vitro culture solution of immature oocytes, and preparation method and application of in-vitro culture solution
  • In-vitro culture solution of immature oocytes, and preparation method and application of in-vitro culture solution

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preparation example Construction

[0056] 2. The preparation method of in vitro culture fluid in embodiment 1~5, comprises the following steps:

[0057] S1. prepare solution I, comprise the following steps:

[0058] 1) Make the required utensils for preparation go through dry heat at 250°C for 30 minutes to remove the heat source;

[0059] 2) Under 100-grade conditions, weigh calcium chloride, potassium chloride, magnesium sulfate, sodium chloride, sodium bicarbonate, sodium dihydrogen phosphate hydrate, arginine, cystine, cysteine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Tryptophan, Tyrosine, Valine, Asparagine, Aspartame amino acid, glutamic acid, N(2)-L-alanyl-L-glutamine, glycine, proline, serine, calcium D-pantothenate (B5), choline chloride, folic acid (B9), Inositol (B8), Niacinamide (B3), Pyridoxal Hydrochloride (B6), Riboflavin (B2), Thiamine (B1), Ascorbic Acid (C), D-Glucose, Sodium Pyruvate, Sodium Lactate and Chin Damycin, and add water and mix uniformly to ...

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Abstract

The present invention relates to the technical field of assisted reproduction and discloses an in-vitro culture solution of immature oocytes. The in-vitro culture solution comprises a basic culture solution and a composition. The basic culture solution comprises a solvent and a solute, the solvent comprises water, and the solute comprises vitamins, energy substances, inorganic salts and amino acids; and the composition comprises 0.01-1 mg / L of butyrolactone-I, 0.001-2 mg / L of estradiol, 0.001-1 IU / L of follicle-stimulating hormone, 0.001-1 IU / L of luteinizing hormone, 0.001-1 IU / L of epidermalgrowth factor, 0.001-1 mg / L of insulin-like growth factor and 0.001-1 IU / L of human chorionic gonadotropin. The present invention also discloses a preparation method and an application of the in-vitro culture solution. In addition to adding basic substances, by adding the energy substances, composition and vitamins, nucleus and cytoplasm of the immature oocytes are synchronously mature and besides, a fertilization rate and a high-quality embryo rate are improved.

Description

technical field [0001] The invention relates to the technical field of assisted reproduction, in particular to an in vitro culture solution of immature oocytes and a preparation method and application thereof. Background technique [0002] In vitro fertilization (in vitro fertilization, IVF), also known as test-tube baby, is a method of assisted reproductive therapy. Specifically, it refers to taking out eggs and sperm, performing in vitro fertilization, culturing and forming embryos, and then transferring the embryos back to the mother's uterus The inner pregnancy develops into a fetus. [0003] Assisted reproductive technology (ART) has come a long way since the first test-tube baby was born in 1978. At first, assisted reproductive technology was to obtain mature eggs in a woman's normal menstrual cycle for in vitro fertilization, but a woman only matured and eliminated one egg in one ovulation cycle. Because of the small number, the success rate of assisted reproductive ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/075
CPCC12N5/0609C12N2500/90C12N2501/11C12N2501/31C12N2500/30C12N2500/38
Inventor 余裕炉张瑜米乐贾晓伟刘洪君严飞戴甄
Owner 成都艾伟孚生物科技有限公司
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