The invention belongs to the field of in-vitro diagnosis, and concretely relates to a
quantum-dot marking test strip capable of quantitatively determining multiple indexes of blood infectious diseases and a preparation method and a
quantitative determination method. A marking pad (3) of the test strip is coated with a
quantum-dot marked mixture of
HBsAg monoclonal antibody, HIVAg, HCVAg and TPAg, a T band (4) of an analysis membrane (7) is coated with a mixture of
HBsAg monoclonal antibody, HIVAg, mouse anti-human
antibody and TPAg, and a C band (5) of the analysis membrane (7) is coated with secondary antibody. The standard curves of all detected substances are stored and installed on the test strip by employing
electron labels. The test strip employs a
detector with
signal detection function to read the standard curves stored in the
electron labels and combines with the to-be-tested sample corresponding
fluorescence intensity measured by the
detector, so as to synchronously quantitatively determine the concentration of
HBsAg, HIVAb, HCVAb and TPAb in the samples.