78results about How to "Increase fixed amount" patented technology

The invention belongs to the technical field of analytical chemistry and chemical sensors and discloses an electrochemical immunosensor for detecting a toxoplasma gondii IgM (Immunoglobulin m) antibody (Tg-IgM) of a gravida and a preparation method of the electrochemical immunosensor. The immunosensor is prepared by sequentially modifying graphene, polythionine, gold nanoparticles and capture antigen to the surface of a glassy carbon electrode. An enzyme-functionalized nano-composite detection probe with an electrical signal amplifying function is prepared by assembling enzyme and a second antibody with high proportions on an Au-Fe3O4 surface. According to the sandwich immunoassay principle, the concentration of Tg-IgM is determined by using an electrochemical signal generated by catalysis of enzyme to a substrate. According to the electrochemical immunosensor, the specificity of immunoreaction is combined with the sensitivity of electrochemical detection; the transmission of electronics is promoted by using the graphene, the polythionine, the gold nanoparticles, Au-Fe3O4 and other material; and the sensitivity of the detection is improved. The electrochemical immunosensor has the advantages of simplicity and convenience for operation, favorable regeneration performance and detection cost reduction. The electrochemical immunosensor prepared on the basis can be also used for detecting other immunological markers and has favorable application prospect in medical diagnosis.

The invention relates to a micro-array biochip, and a preparation method and an application of the micro-array biochip. The micro-array biochip comprises a glass chip substrate and a modification layer. The modification layer comprises a chromium layer, a silver and / or gold layer, a self-assembly monomolecular layer, and a photo-cross-linking agent and high-molecular layer, which are overlaid in sequential on the surface of the glass chip substrate. The invention also provides a protein micro-array, a method for preparing the protein micro-array, and an application of the protein micro-array to detect micromolecule medicine. According to the invention, three-dimensional high-molecular surface modification of the micro-array biochip is completed by the method, and the fixation amount of protein on the surface of the micro-array biochip can be effectively improved, so that micromolecule, hard to detect in the prior art, can be detected without marking, in accuracy, and in high flux. The micro-array biochip is of great significance to the field, such as molecular biology study and medicinal development.

The invention discloses a surface modification method of a biomaterial magnesium alloy. The method comprises, firstly, performing surface chemical treatment on the surface magnesium alloy; secondly, self-assembling and immobilizing amino silane molecules; lastly, sequentially immobilizing polyethylene glycol, fibronectin and heparin on the surface to structure a multifunctional bioactive surface and meanwhile to improve the physiological corrosion-resistant performance of the magnesium alloy. Through surface modification of the biomaterial magnesium alloy, the method can improve the physiological corrosion-resistant performance of the magnesium alloy and endow the material with good blood compatibility and endothelial cell growth promoting property, thereby laying a good foundation for application of the biomaterial magnesium alloy to the fields of intravascular implantation of materials or devices such as intravascular stents.

The invention relates to an electrochemical immune sensor for phosphating protein. In the electrochemical immune sensor, a magnetic nanometer-antibody composition MPs-p53<15>Ab1 is used as an adsorbent to separate the phosphating protein, a carbon nanometer sphere CNS is used as a carrier, lead phosphate-apoferritin (LPA) and a phosphating protein antibody p5315Ab2 are modified on the surface of the CNS, and thus, LPA-p53<15>Ab2-CNS nanometer complexing agent is prepared for detecting signal amplification. According to the sandwich immunoassay principle, the content of the phosphating protein to be detected is in proportion to the captured LPA-p5315Ab2-CNS, and the content of lead ions encapsulated in LPA is detected according to a dissolving volt-ampere method to measure the concentration of the phosphating protein in a sample. The method has the advantages of simplicity and convenience for operation and simple and efficient separation, and the advantage that an integral immunologic reaction is finished in an epoxy (EP) tube; a large number of LPA signal molecules are introduced by taking the CAN as the carrier, and each LPA molecule contains a large number of Pb<2+>, so the sensitivity of detection is improved; and the dissolved Pb<2+> is used as a detection signal without adding an enzyme substrate, so the electrochemical immune sensor is a reagent-free sensor. In the electrochemical immune sensor for the phosphating protein, the linear concentration range of the phosphating protein phosphor-p53<15> is from 0.02 to 20 ng mL<-1>, and the detection limit is 0.01 ng mL<-1>.

The invention discloses a preparation method for an immunosensor based on gamma-polyglutamic acid grafted dopamine and chitosan complex micelles. The preparation method comprises the following steps: preparing polymeric micelles; preparing polymeric micelle modified electrodes; preparing the immunosensor. According to the preparation method, the prepared polymeric micelles are very good in biocompatibility, the application of the polymeric micelles to the immunosensor can effectively increase the immobilization amount of antibodies and well keep the activity of biomacromolecules, and the prepared immunosensor has the advantages of high specificity, good stability, wide detection range and the like; the combination of a macromolecular self-assembly technology and an electrochemical sensor can be widely applied to immunoassay and extendedly applied to the fields of food safety, biological medicines, environmental protection monitoring and the like.

The invention discloses a fly-ash-based carbon dioxide curing agent and a preparation method thereof. The fly-ash-based carbon dioxide curing agent comprises fly ash, slaked lime and aluminum oxide, wherein the weight ratio of fly ash to slaked lime to aluminum oxide is (3-4.5):(1.5-3):2. When the ratio of fly ash to slaked lime is 1:1, the carbon fixation amount is highest, and due to doping of the slaked lime, the leaching toxicity of fly ash after carbonation is reduced, and the fly-ash-based carbon dioxide curing agent is mainly used for leaching of heavy metals;fly ash is a coal burning product, is one of main bulk wastes in China at present, greenhouse-gas carbon dioxide is sealed up for storage by adopting bulk-solid-waste fly ash and the purpose of using wastes to treat wastes is achieved.

The invention discloses a microorganism sample rapid detection method and a detection device thereof, which is realized by a mesoporous biochip. The detection method comprises the following steps: firstly fixing antibodies on micropore tunnel surfaces of a mesoporous biochip, allowing a test sample to flow through the chip micropore tunnels with the antibodies fixed so as to realize separation and enrichment of pathogenic microorganisms or other biological substances by the chip, then injecting a solution containing a fluorescence-labeled or an enzyme-labeled antibody into the chip micropore tunnel to form a sandwich-type antibody-antigen-labeled antibody immune complex, and detecting the pathogenic microorganisms or other biological substances by detecting the fluorescence intensity of the fluorescence-labeled antibody or the light absorption or chemiluminiscence intensity of the product of the enzymic catalytic reaction so as to realize the detection of the microorganisms to be detected. The mesoporous biochip has a great number of micropore tunnels, which greatly increases the reaction area, and thus when used for microorganism sample detection, the detection method and the detection device of the invention have the characteristics of high detection sensitivity and high detection speed.

The invention discloses preparation and an application of an electrochemical luminescence sensor for detecting procalcitonin based on energy transfer between g-C3N4 and CuO, and belongs to the technical field of construction of novel sensors. Based on the good specificity between antigen and antibody, the sensor utilizes Au and carbon nanotube modified g-C3N4 composite material g-C3N4-CNT@Au as asubstrate luminescent material and polydopamine coated CuO as a quenching agent, and the electrochemical luminescence sensor is constructed through layer-by-layer self-assembly. The electrochemical luminescence sensor constructed by the invention has a wider detection range, higher sensitivity and lower detection limit, and has important significance for detection of procalcitonin.

The invention discloses a preparation method of a multi-growth-factor slow release coating of a titanium and titanium alloy surface. According to the method, a plurality of layers of coatings containing nanoparticles of growth factors are prepared on the titanium surface by taking the nanoparticles as a growth factor carrier and charged high molecular thin films as a medium. The nanoparticles included in the coating are prepared by mixing polycation solution containing dopamine of certain concentration with polyanion solution containing the growth factors. The nanoparticles have good dispersibility, can protect the activity of the growth factors and are easy to fix on the surface of a material. By controlling the electric property of the nanoparticles and the high molecular thin films, nanoparticles carrying with different factors are embedded between the high molecular thin films. By adjusting the loading quantity and the loading sequence of different nanoparticles and the thickness of the coatings, effective control over release speed, release quantity and release sequence of the various growth factors can be realized.

The invention discloses a method for improving concrete cement paste chlorine ion combination long residual action, and belongs to the technical field of building materials. The method comprises the following steps: adding ferric hydroxide and silicon ash to cement paste, and doping triethanolamine or triisopropanolamine, wherein a total mass of a cement drier, the ferric hydroxide and the siliconash is 100%, wherein the ferric hydroxide is 5%-10%, the silicon ash is 2%-5%, the triethanolamine accounts for 0.01%-0.1% of a total mass of the above three parties, and the triisopropanolamine accounts for 0.0025%-0.1% of the total mass of the above three parties. The method is capable of improving chlorine ion fixation capacity of cement concrete, widening an application of the triethanolamineand the triisopropanolamine in the cement concrete, and relieving a corrosion problem of a reinforcing steel bar because of the action of chlorine ions in seawater.