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Microorganism sample rapid detection method and detection device thereof

A detection device and detection method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complex operation, high price, large equipment, etc., and achieve the effect of improving sensitivity and increasing the reaction surface area

Active Publication Date: 2013-01-16
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] For the current situation of the rapid detection technology of microorganisms in the prior art, the purpose of the present invention is to provide a new rapid detection method for microorganisms and a rapid detection device for its implementation, so as to overcome the limitations of the existing rapid detection technology for microorganisms. Qualitative determination, low detection sensitivity, complex operation, time-consuming and laborious, large equipment, expensive, not suitable for field operations, etc.

Method used

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  • Microorganism sample rapid detection method and detection device thereof
  • Microorganism sample rapid detection method and detection device thereof
  • Microorganism sample rapid detection method and detection device thereof

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Embodiment 1

[0036] In this embodiment, the structure of the detection device for rapid detection of biological samples is as follows figure 1 and image 3 As shown, the detection principle is as attached Figure 4 shown. The structure of the detection device mainly includes a measurement light source 7, a sample solution storage tank 1 to be tested, a buffer solution storage tank 2, a labeled antibody solution storage tank 3, a catalytic reaction solution storage tank 4, a multi-way control valve 5, a sampling pump 6, an intermediate Pore ​​biochip sensor, waste liquid pool 12, outgoing light focusing lens 9, photodetector 10 and signal processing controller 11, described mesoporous biochip sensor is made up of mesoporous biochip 8 and the liquid inlet that is positioned at both sides of mesoporous biochip chamber and liquid collection chamber 25, the microporous channel 26 on the mesoporous biochip connects the liquid inlet chamber with the liquid collection chamber, the liquid inlet c...

Embodiment 2

[0040] The detection device and detection method used for rapid detection of biological samples in this embodiment are basically the same as in Example 1, except that the detection device for rapid detection of biological samples is only provided with a liquid storage tank for detection, and the mesoporous The biochip is a combined structure, as attached figure 2 As shown, it consists of a mesoporous biochip body and mesoporous material sheets evenly distributed and fixed on six areas around the mesoporous biochip body, and different antigens are immobilized on the micropore surfaces of the mesoporous material sheets in different areas. Detection uses fluorescent reagents as labeled antibodies. When the detection is carried out, the sample solution to be tested is first injected into the sample solution storage tank to be tested, and then the solution to be tested is pumped into the micropore channel flowing through the mesoporous biochip. After the immune reaction on the su...

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Abstract

The invention discloses a microorganism sample rapid detection method and a detection device thereof, which is realized by a mesoporous biochip. The detection method comprises the following steps: firstly fixing antibodies on micropore tunnel surfaces of a mesoporous biochip, allowing a test sample to flow through the chip micropore tunnels with the antibodies fixed so as to realize separation and enrichment of pathogenic microorganisms or other biological substances by the chip, then injecting a solution containing a fluorescence-labeled or an enzyme-labeled antibody into the chip micropore tunnel to form a sandwich-type antibody-antigen-labeled antibody immune complex, and detecting the pathogenic microorganisms or other biological substances by detecting the fluorescence intensity of the fluorescence-labeled antibody or the light absorption or chemiluminiscence intensity of the product of the enzymic catalytic reaction so as to realize the detection of the microorganisms to be detected. The mesoporous biochip has a great number of micropore tunnels, which greatly increases the reaction area, and thus when used for microorganism sample detection, the detection method and the detection device of the invention have the characteristics of high detection sensitivity and high detection speed.

Description

technical field [0001] The invention relates to a microbial sample detection technology, in particular to a method for rapid detection of biological samples using a mesoporous biochip, and a rapid detection device for microbial samples for implementing the method. Background technique [0002] Pathogenic microorganisms are invisible hazards and have always been a hot issue of concern. Their pollution is a major safety issue and seriously affects human health. According to estimates by the World Health Organization, billions of people around the world are infected with diseases due to pathogenic microorganisms every year. The incidence rate in developed countries is also quite high, with an average of 1 / 3 of the population infected each year. Escherichia coli O157:H7 pollution occurred in Jiangsu, Anhui and other places in my country in 2001, causing 20,000 people to be poisoned and 177 people to die. Salmonella causes hundreds of poisoning outbreaks every year. In Shangha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
Inventor 段忆翔于巧玲
Owner SICHUAN UNIV
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