33results about How to "Inhibition of apoptosis" patented technology

The invention provides a multifunctional nano-biological material transfection reagent with the effect of gene therapy for Parkinson's disease and a preparation method thereof. The method is to immobilize nerve growth factor (NGF) on hydrogel-wrapped iron ferric oxide nanoparticles (Fe 3 o 4 -OA-NAPI-AA) surface, and then adsorb plasmid DNA that can interfere with α-synuclein synthesis. The nanomaterial transfection reagent acts on MPP + After induction, the apoptosis of PC12 cells, an in vitro Parkinson's disease model, was significantly inhibited, the expression of nerve growth factor receptor (NGFR) was up-regulated, the expression of α-synuclein was down-regulated, and the classic BAX, P53, Bcl-2 apoptosis pathway to achieve the purpose of treating Parkinson's disease. The nano material transfection reagent of the present invention is non-toxic, has few side effects, can successfully carry genes into cells, and has obvious effects, and is worthy of popularization and application in the treatment of Parkinson's disease.

The invention provides an application of peroxy-ergosterol in preparation of a drug for preventing or treating influenza virus infection, and provides a method for separating peroxy-ergosterol from isatis roots simultaneously. The application of a small molecular compound peroxy-ergosterol in preparation of the drug for preventing or treating the influenza virus infection is provided by the invention, so that the effective drug for preventing and treating clinical influenza currently is provided. The inventor finds that peroxy-ergosterol can effectively inhibit influenza virus induced host cells from overexpressing a pattern recognition receptor RIG-I for pathogen recognition, so that effects of abnormal immune inflammation and mediated apoptosis transduced and mediated with pattern recognition receptor downstream signals are inhibited.

The invention relates to a method for preparing a sappan wood extract, in particular to a method for preparing an irrigating solution with an anti-tumor function. The method solves the problems of extracting sappan woods with specific effects in the prior art. The method comprises the following steps: weighing a certain amount of crushed sappan wood, adding purified water which is 3 to 5 times ofthe crushed sappan wood into the crushed sappan wood, soaking the crushed sappan wood for 12 to 24 hours at the room temperature, boiling the mixture for 30 minutes and separating an extract; adding water into the crushed sappan wood again, boiling the mixture for 30 minutes, extracting the mixture, and separating the extract; adding the purified water into the crushed sappan wood again, boiling the mixture for 30 minutes, extracting the mixture, and separating the extract; and combining the three extracts, performing vacuum concentration on the combined extract until the amount is 12 to 16 percent of the total amount of the added water, freezing and icing the concentrated solution at a temperature of 20 DEG C below zero, taking the frozen and iced concentrated solution out to unfreeze the concentrated solution naturally at the room temperature, filtering the unfrozen concentrated solution to remove deposits, heating and boiling the concentrated solution obtained in the previous step again, freezing and icing the concentrated solution at a temperature of 20 DEG C below zero, taking the frozen and iced concentrated solution out to unfreeze the concentrated solution naturally at theroom temperature, and filtering the unfrozen concentrated solution to remove the deposits. The obtained product has significant anti-tumor effect.

The invention discloses a medicine for treating skin squamous cell carcinoma and a preparation method thereof. The weight ratio of raw materials is as follows: 4-6 parts of pulsatilla, 2-4 parts of curcuma, and 1-3 parts of ginger. Add 12 times the weight of the medicinal materials and extract with 70% ethanol three times, each time for 2 hours; combine the filtrates, concentrate them into a 1:1.3 liquid extract, dry them in a 65-75°C thermostat until they become hard and brittle, dry and store at low temperature. have to. The invention can significantly inhibit the proliferation of skin squamous cell carcinoma A431 cells, and at the same time, it also has the obvious effect of inducing the apoptosis of A431 cells, and can prevent the cells from staying in the G0 / G1 phase, hinder the DNA synthesis in the S phase, and inhibit tumor cells proliferation.

The invention relates to the technical field of medicine synthesis, and provides a cholic acid derivative as well as a preparation method and application thereof. The cholic acid derivative provided by the invention has a remarkable effect of inhibiting hepatocyte apoptosis, can effectively inhibit hepatocyte damage induced by glycochenodeoxycholic acid, and the inhibition rate of part of the derivative is superior to that of a positive control tauroursodeoxycholic acid; the cholic acid derivative provided by the invention provides a reference for research and development of liver protection drugs, and has a wide application prospect in preparation of the liver protection drugs.