The invention relates to the field of
assisted reproductive technology, and discloses a composition and application thereof, and a
sperm refrigerating fluid and a preparation method thereof. The spermrefrigerating fluid comprises a basic culture solution, the composition, a
cryoprotectant,
human serum albumin and a bactericide, wherein the composition is prepared from
resveratrol with a concentration of 12 to 26mg / L, reduced
glutathione with a concentration of 0.2 to 1.9 mg / L ,
lecithin with a concentration of 436 to 775 mg / L,
cholesterol with a concentration of 0.5 to 1.6 mg / L, an ATP sodiumsalt with a concentration of 9 to 17 mg / L,
heparin with a concentration of 11 to 28 mg / L,
phosphatidylserine with a concentration of 93 to 299 mg / L and coenzyme Q10with a concentration of 5 to 25 mg / L; and the
cryoprotectant comprises
paclitaxel with a concentration of 0.3 to 1.1 mg / L of,
trehalose with a concentration of 16 to 22 g / L,
cane sugar with a concentration of 7 to 13 g / L and
glycerol with a concentration of 80 to 250 mL / L. The
sperm refrigerating fluid can reduce damage of sperms in low-temperature
refrigeration and in freezing
recovery, improve
vitality of the sperms after freezing
recovery, prolong the survival time of the sperms after freezing
recovery, promote
sperm capacitation and enhance penetrating fertilization capacity of the sperms to egg cells; and thus, the fertilization success rate and
blastocyst formation rate of the ova are improved, and finally, the effect of improving the
pregnancy success rate of an in-vitro fertilization assisted
reproduction technologyis achieved.