Cryoprotectant and application thereof, sperm refrigerating fluid and preparation method of sperm refrigerating fluid
A cryoprotectant and freezing fluid technology, applied in the field of assisted reproduction, can solve the problems of limited sperm cryoprotection, the appearance of granular substances, and the impact on the processing process, so as to protect from oxidative damage, improve productivity, and improve fertilization. Effect
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[0059] 2. The preparation method of sperm freezing liquid in embodiment 1~3, comprises the following steps:
[0060] S1. Under 100-level conditions, weigh reduced glutathione, ATP sodium salt, heparin, trehalose, sucrose, sodium chloride, potassium chloride, calcium chloride, magnesium sulfate, potassium dihydrogen phosphate, Glucose, amino acids, alanyl glutamine, sodium lactate, sodium pyruvate, taurine, HEPES and MOPS were added to water and mixed uniformly to obtain solution I;
[0061] S2. Weigh resveratrol, coenzyme Q10, paclitaxel and cholesterol according to the proportion, add them to ethanol and mix evenly to obtain solution II; wherein, the concentration of resveratrol, coenzyme Q10, paclitaxel and cholesterol in solution II is the final concentration 1000 times of;
[0062] S3. Weigh lecithin, phosphatidylserine and glycerin according to the proportion, and mix them uniformly to obtain solution III;
[0063] S4. Stirring and dropping solution II into solution III...
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