The invention provides a human sperm cryopreservation liquid, a human sperm cryopreservation method, and a human sperm cryopreservation and recovery method. The cryopreservation liquid comprises a yolk liquid, glycerol, antibiotics, a refrigeration buffer solution, and quercetin or derivative thereof. The human sperm cryopreservation and recovery method comprises the steps of using the human sperm cryopreservation liquid, performing cold balance at a temperature of 4 DEG C, and then performing refrigeration at a temperature of minus 80 DEG C, and next, transferring to a liquid nitrogen tank for cryopreservation; and performing unfreezing and recovering in a water bath box at a temperature of 37 DEG C before use. The sperm cryopreservation liquid, added with the quercetin at a proper concentration, can effectively protect sperms and can remarkably improve survival rate and motility of revived sperms in the subsequent sperm recovery process, so that occurrence of sperm ROS and apoptosis can be reduced; and therefore, the human sperm cryopreservation liquid has application potential in the assisted reproductive technology.