Sperm cryoprotectant and preparation method thereof

A cryoprotectant and cryopreservation technology, which is applied to the preservation, application, and animal husbandry of human or animal bodies, can solve problems such as infection and complex components of cryoprotectants, and achieve the effect of simple preparation methods

Inactive Publication Date: 2016-05-04
REPRODUCTIVE & GENETIC HOSPITAL OF CITIC XIANGYA CO LTD
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the sperm cryoprotectants used in various sperm banks and reproductive centers are mostly formulated with egg yolk, glycerin, and sucro...

Method used

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  • Sperm cryoprotectant and preparation method thereof
  • Sperm cryoprotectant and preparation method thereof

Examples

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Embodiment 1

[0028] A kind of cryoprotectant for sperm, its formula composition and content are as follows:

[0029] Sucrose 16g, sodium bicarbonate 0.5g, glycerin 36mL, 20% (g / mL) human albumin 25mL, five-distilled water 60mL.

[0030] The preparation method of above-mentioned sperm cryoprotectant comprises the following steps:

[0031] 1. Weigh 16g of sucrose and 0.5g of sodium bicarbonate on an electronic balance;

[0032] 2. Measure 36mL of glycerin, 25mL of 20% (g / mL) human serum albumin, and 60mL of five-distilled water (made by conventional methods, obtained by distilling water five times) in a graduated cylinder for use;

[0033] 3. First add 60mL five-distilled water into a 250mL beaker, and then put stirring magnetic beads;

[0034] 4. Place the beaker on the magnetic stirrer, first adjust the speed to the lowest, turn on the power, and then adjust the speed to an appropriate speed, so as to maintain a large speed and the liquid in the beaker will not splash out;

[0035] 5. A...

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Abstract

The invention relates to a sperm cryoprotectant and a preparation method thereof. Every 125 ml of the sperm cryoprotectant is prepared from saccharose, sodium bicarbonate, glycerol, human serum albumin and water obtained by distilling for five times. The novel sperm cryoprotectant does not contain yolk and only contains saccharose, sodium bicarbonate, glycerol, 20% (g/mL) human serum albumin and other commonly-used reagents with definite chemical components, the risk of animal source pathogenic bacterium infection does not exist, the acquired freezing effect is similar to that of an existing formulation, and the average value of the sperm freezing-thawing rate is about 75% to 80%.

Description

technical field [0001] The invention relates to the field of sperm cryopreservation in assisted reproductive technology, in particular to a sperm cryoprotectant and a preparation method thereof. Background technique [0002] The cryopreservation of human sperm plays an important role in human reproductive health, and the cryoprotectant added during the freezing process is one of the key factors affecting the effect of cryopreservation. [0003] At present, the sperm cryoprotectants used in various sperm banks and reproductive centers are mostly formulated with egg yolk, glycerin, and sucrose. Since egg yolk must be added, the composition of the cryoprotectant is quite complicated, and there is a risk of animal-derived pathogenic bacteria infection. Contents of the invention [0004] Based on this, it is necessary to provide a sperm cryoprotectant and a preparation method thereof in order to solve the above problems. The sperm cryoprotectant of the present invention does no...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 朱文兵卢光琇范立青彭向杰
Owner REPRODUCTIVE & GENETIC HOSPITAL OF CITIC XIANGYA CO LTD
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