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191 results about "Prenatal development" patented technology

Prenatal development is the process in which an immature individual develops before birth. For instance, mammalian prenatal development is the process where an embryo or fetus gestates during pregnancy, from fertilization until birth. Prenatal development starts with fertilization, which marks the beginning of embryogenesis. Embryogenesis, in turn, continues in fetal development, which lasts until birth.

Compounds, methods, and treatments for abnormal signaling pathways for prenatal and postnatal development

InactiveUS20090214474A1Prevent and reduce rateBiocideOrganic chemistryBiological bodyPhysiology
The present invention relates to prevention of congenital deformations. The invention further relates to cancer inhibition and prevention. The invention further relates to methods and compositions to modulate, antagonize, or agonize disparate signaling pathways that may converge to regulate patterning events and gene expression during prenatal development, post-natal development, and during development in the adult organism.
Owner:JENNINGS BARBARA BROOKE

Methods for in vitro fertilization

Instead of immersing human reproductive cells in a single culture medium throughout the various procedures used in IVF, a process is provided by which the reproductive cells may be moved through a sequence of distinct culture media as the various IVF procedures are carried out. In one implementation, the culture media specifically formulated to provide a physical environment similar to that found within the female reproductive tract and conducive to growth and development of human reproductive cells during the various stages of the IVF process. In this regard, specifically formulated culture media can be applied to support the reproductive cells in one or more of the following procedures: oocyte retrieval and handling; oocyte maturation; ordinary fertilization; oocyte, zygote and embryo examination and biopsy; embryonic development to the eight-cell stage; embryonic development to the blastocyst stage; embryo transfer; and cryopreservation.
Owner:VITROLIFE AB

Methods and apparatus for assessing embryo development

A method of processing a series of images of an embryo to determine estimated timings of developmental events for the embryo, wherein the method comprises: determining feature information for each image, the feature information for each image representing the content of the image; establishing machine learned classifiers for associating each image with a respective likelihood of the image being associated with one or more developmental events based on the feature information for the image; applying the machine learned classifiers to the feature information for each image to determine a respective likelihood of the image being associated with one or more developmental events, and determining estimated timings for the plurality of developmental events for the embryo from the respective likelihoods of the respective images being associated with respective ones of the plurality of developmental events. The method may further comprise determining an indication of a confidence estimate for the timings.
Owner:VITROLIFE AS

Fetal stimulation device

A fetal stimulation device is incorporated into a garment to be worn by a pregnant woman. The garment is preferably formed of an elastic material and includes a torso portion which is arranged around the torso of the woman. An audio device and a pair of speakers are connected with the garment torso portion to deliver sound such as music or words to a fetus. The garment torso portion also includes and elongated massaging element which is arranged around the perimeter of the torso portion to deliver vibrations to the fetus. The sounds and vibrations stimulate the fetus during prenatal development. In addition, massaging and heating elements are provided in the rear of the garment to deliver vibrations and heat to the back of the woman. A layer of radiation proof fabric is provided between the audio device and the woman to prevent radiation from reaching the fetus.
Owner:PHRONESIS

Preparation method and application of tissue slice for observing temporal-spatial distribution of early embryo development in vivo

InactiveCN102944456AObservation continuityEasy to observe continuityPreparing sample for investigationCooking & bakingFluorescence
The invention discloses a preparation method and an application of a tissue slice for observing temporal-spatial distribution of early embryo development in vivo. The preparation method comprises the following steps that 4% paraformaldehyde fixing, upward gradient ethanol dehydration, wax dipping, embedding, serial section, baking, dewaxing and downward gradient ethanol rehydration are performed in sequence on oviducts or uterine tissues which contain mice embryos in every period, and finally, after haematoxylin-eosin staining is performed on the tissues in the slice, neutral gum is used for sealing the slice, or after immunofluorescence histochemical staining is performed on the slice, a fluorescence resistant quenching sealing agent is used for sealing the slice. The tissue slice disclosed by the invention can be used for manufacturing a map of early mice embryo development and detecting the expression of Crb3 in the mice embryos in every period of development in vivo. The preparation method has the advantages that positions of all organs in the embryos can be relatively fixed, so that the position change of embryo cells in a genital tract and the continuity of embryo development can be conveniently observed, the structure is clear, and the tissue slice is convenient to store.
Owner:NORTHWEST A & F UNIV

Male trait inducer and induction method for pelodiscus sinensis in embryonic periods

The invention relates to a male trait induction method for pelodiscus sinensis in embryonic periods. The male trait inducer is characterized in that through dissolving an aromatase inhibitor and an adjuvant into alcohol with the purity of 92-98%, the inducer with an aromatase inhibitor concentration of 50-150 mu g / mu l and an adjuvant concentration of 50-150 mu g / mu l is obtained; and the adjuvant is selected from testosterone, methyltestosterone or testosterone propionate. According to the induction method provided by the invention, the male induction rate can be more than 95% just through spraying the inducer two times at the sex determination key time point in embryonic development, and meanwhile, the normal development and low drug residues of pelodiscus sinensis gonads are ensured; and the induction method provided by the invention is easy to operate, low in cost, and remarkable in economic benefit, thereby being a sex control technology which is worthy of popularization in the existing pelodiscus sinensis breeding.
Owner:ZHEJIANG WANLI UNIV

Conditional Cas9 expression induced swine trophoblastic cell line and establishment method and application thereof

InactiveCN105624194ATo achieve the purpose of conditional knockoutEasy to operateEmbryonic cellsFermentationPregnancyEmbryo
The invention discloses a conditional Cas9 expression induced swine trophoblastic cell line and an establishment method and application thereof. The method includes: subjecting a lentiviral vector, for controlling Cas9 gene expression, of a Tet-on system to virus packaging, concentrating and purifying; incubating lentivirus with a typical swine trophoblastic cell line, changing fluid, adding puromycin for drug screening, diluting cells to single ones, and culturing to obtain the single-cell-source swine trophoblastic cell line with Cas9 expression under Tet-on regulation. The swine trophoblastic cell line is simple in operation and convenient to use and has a potential utilization value in researching of key functional genes of some trophoblast cells and screening of swine gene target spots. The cell line is expected to be a significant cell material for researching swine placenta development and applicable to correlation researches on swine early-stage placenta development and uterine pregnancy mechanisms and also has a high reference value in research of human early embryonic development.
Owner:AGRO BIOLOGICAL GENE RES CENT GUANGDONG ACADEMY OF AGRI SCI

Gene of wintersweet late embryogenesis abundant protein (LEA) and low-temperature resistant application thereof

InactiveCN102010869AImprove the ability to resist low temperatureImprove low temperature resistanceBacteriaMicroorganism based processesLate embryogenesis abundant proteinsExpression vector
The invention relates to a gene of wintersweet late embryogenesis abundant protein (LEA) and low-temperature resistant application thereof, belonging to the field of molecular biology and gene engineering. The invention provides a gene of wintersweet LEA and protein and also provides an expression vector and a host cell corresponding to the gene. The invention has the advantage that the application of the gene of the wintersweet LEA can improve the low-temperature resistance of a strain.
Owner:JILIN UNIV

Reeves shad parent fish nutrition reinforcement forage and its preparation method

The invention provides a Reeves shad parent fish nutrition reinforcement forage. The Reeves shad parent fish nutrition reinforcement forage is composed of the following raw materials, by weight, 30 parts of an artificial compound forage, 35 parts of fresh trash fish, 20 parts of clam, 15 parts of nereid, 0.5 parts of vitamin C, 0.5 parts of vitamin E, 0.5 parts of fish oil, and 0.1 part of soybean lecithin. The Reeves shad parent fish nutrition reinforcement forage has the following technical effects: 1, a bait palatability problem is solved, and parent fish can effective take various reinforcement nutrients; and 2, the addition of a proper amount of the fish oil can effectively provide various fatty acids required by the breeding of the parent fish and does not cause over-nutrition, VC and VE can effectively prevent the oxidation of the fatty acids in the parent fish, remove excess free radicals generated in the body and enhance the fish immunity, VE can also promote the sexual hormone secretion and improve the fecundity of the parent fish, and lecithin is helpful for adjusting the nutrient digestion and absorption of the parent fish, is an energy source in the embryonic development phase and can also participate in the synthesis of fish body fats of larva fish.
Owner:江苏中洋动物营养科技有限公司

In-vitro culture solution for culturing swine parthenogenetic activated embryos and swine in-vitro fertilized embryos

InactiveCN103710299AImprove developmental efficiencyIncrease the number ofEmbryonic cellsCulture fluidEmbryo
The invention discloses a swine in-vitro embryo culture solution. The swine in-vitro embryo culture solution disclosed by the invention is obtained through adding ligustrazine, with the final concentration of 0.05-5.00 microgram / milliliter, into an embryo culture solution NCSU-23. A traditional Chinese medicine monomer component, namely ligustrazine, is added into the swine in-vitro embryo culture solution disclosed by the invention; shown by experiments, through carrying out in-vitro culture on the swine parthenogenetic activated embryos and the swine in-vitro fertilized embryos by using the culture solution, the development efficiency of the embryos and the number of the cell masses and total cells in blastulae can be effectively increased. The swine in-vitro embryo culture solution has the advantages that a foundation is laid for the development of other biotechnologies, medical research and animal husbandry, and meanwhile, a certain theoretical basis for clarifying the development mechanism of early swine embryos is provided.
Owner:BEIJING UNIV OF AGRI

Method for improving blastocyst rate of in-vitro embryos of animals

InactiveCN102703377AImprove developmental abilityImprove early developmental abilitiesEmbryonic cellsCulture fluidEmbryo
The invention provides a method for improving the blastocyst rate of in-vitro embryos of animals. According to the method, the developmental capacity of a parthenogenetic embryo and an in-vitro fertilization embryo can be remarkably improved by adding type I interferon into an embryonic development culture solution for the in-vitro culture of the embryos; compared with the blastocyst rate obtained under the culture of the conventional culture solution, the blastocyst rate is improved by 7 to 13 percent; and the blastocyst hatching rate is also remarkably higher than the blastocyst hatching rate under the culture of the conventional culture solution. By the method, the early development capacity of in-vitro embryos of cattle and sheep is improved, so that the in-vitro production efficiency of embryos of the cattle and sheep is improved, and high-quality embryos are provided for the scientific research and production field in which the embryos of the cattle and sheep are taken as materials.
Owner:CHINA AGRI UNIV

Poultry egg embryo incubation and growth process monitoring system

At present, when the poultry egg embryo grows in an incubator, embryos die in batch during growth due to environment factor mutation because the incubator does not have a monitoring system for the growth process so as to cause huge economic loss. The invention designs a set of measuring system for obtaining the fetal movement signal of a living body poultry egg embryo in the incubation period and provides a new method for measuring the fetal movement of the poultry egg embryo with a semi-intrusion method. On the basis of the flow equation of continuity of flow medium hydraulic transmission, the method utilizes a displacement sensor to extract the fetal movement signal of a living body poultry egg embryo in the incubation period (shown in figure one); under the condition that the poultry egg growth is not affected, the invention realizes the monitoring of the whole process of the poultry egg embryo growth in the incubation period. If the heartbeat signal of the living body embryo weakens or is abnormal in the incubation and growth process, the control system of the incubator immediately checks the incubator and correspondingly adjusts the working parameter so as to realize quick hidden danger and fault treatment to enable the incubator to work normally, thus avoiding serious economic loss. The invention of the monitoring system plays an important role in researching hatching egg growth process, increasing poultry hatching rate, sorting and improving the product quality of poultry egg in the biological pharmacy production and has great development prospect.
Owner:QINGDAO TECHNOLOGICAL UNIVERSITY

Embryo division process analysis and pregnancy rate intelligent prediction method and system

The invention discloses an embryo division process analysis and pregnancy rate intelligent prediction method and system. The method comprises the following steps: collecting embryo images within D1 toD6 periods; inputting the embryo image into a prokaryotic number prediction network model, a blastomere number prediction network model, a fragment proportion prediction network model, a blastocyst cavity and inner cell mass grade prediction network model and a trophoblast grade prediction network model; calculating and outputting a predicted prokaryotic number, a predicted blastomere number, a predicted fragment proportion, a predicted blastocyst cavity proportion, a predicted intracellular mass grade and a predicted trophoblast grade of the embryo image; and inputting the same into an embryo pregnancy rate state prediction machine learning model to calculate and output an embryo pregnancy rate prediction result. According to the intelligent prediction method and system, the whole embryodevelopment process is monitored, the embryo pregnancy rate is obtained through calculation by means of the comprehensive scoring function, manual intervention is not needed in the prediction process, and doctors can be helped to quickly and accurately judge embryo scores.
Owner:WUHAN MUTUAL UNITED TECH CO LTD

Device and method for observing and recording chicken incubation experiment

The invention relates to a device and method for observing and recording a chicken incubation experiment. The device comprises a processor, a camera, an egg irradiating device, an incubation support plate, a warmer, a humidifier, a fan, a steering engine, a temperature sensor, a humidity sensor and an oxygen concentration sensor, wherein the processor has a timing function and a timing processing function, the camera is connected with the processor and used for acquiring the images of eggs, the egg irradiating device is connected with the processor and used for irradiating the eggs, the incubation support plate is used for bearing the eggs, the warmer is connected with the processor and used for adjusting environment temperature, the humidifier is connected with the processor and used for adjusting environment humidity, the fan is connected with the processor and used for adjusting environment oxygen concentration, the steering engine is connected with the processor and used for driving the incubation support plate to rotate, the temperature sensor is connected with the processor, the humidity sensor is connected with the processor, and the oxygen concentration sensor is connected with the processor. By the device and method for observing and recording the chicken incubation experiment, an experimenter can clearly observe the development process of egg embryos and monitor and record the development conditions of the embryos in real time, and cost can be saved.
Owner:蔡海睿

Compounds, methods, and treatments for abnormal signaling pathways for prenatal and postnatal development

InactiveUS20110218176A1Inhibits cancer growthAvoid survivalBiocideMetabolism disorderDiseaseBiological body
The present invention relates to prevention of congenital deformations. The invention further relates to cancer inhibition and prevention. The invention further relates to methods and compositions to modulate, antagonize, or agonize disparate signaling pathways that may converge to regulate patterning events and gene expression during prenatal development, post-natal development, and during development in the adult organism. The invention also relates to activators or deactivators of pyruvate kinase M2 (PKM2) for the treatment, prevention, or amelioration of diseases related to PKM2 function.
Owner:JENNINGS SPRING BARBARA BROOKE

Octopus minor oosperm collector and application thereof

The invention discloses an octopus minor oosperm collector and a large-scale octopus minor oosperm collecting and incubating method. A PVC straight round tube serves as the place for parent spawning, one end of the PVC straight round tube is sealed with a mesh sheet and a rubber band to form a sealed end, multiple oosperm collectors are arranged at the sealed end and the unsealed end alternately and are bound with a hemp rope into a series connection structure, and the oosperm collectors serve as both a parent octopus minor oosperm collector and a shelter. After a female octopus minor and a male octopus minor are placed into a spawning pond, the female octopus minor spawns on the wall of the PVC straight round tube, and the unsealed ends of the octopus minor oosperm collectors are sealed with the mesh sheet after spawning so that the oosperms can be collected; then the oosperms are moved to an incubation pond for incubation, and the mesh sheet is replaced when embryonic development of the oosperms reaches the second overturning period. One end of each oosperm collector is sealed with the mesh sheet to allow the parent to hide inside, so that only the other end needs to be watched over, physical power is saved, the parent is effectively isolated, and in this way, the spawning parent octopus minor is protected from disturbance of other parents and prevented from abandoning eggs after being disturbed.
Owner:OCEAN UNIV OF CHINA

Therapies and compositions for controlling the stress mechanism and for stabilizing hemostasis in an organism

A theory has been presented that provides a simplified explanation of a cohesive mechanism of embryological development, hemostasis, coagulation, wound repair and tissue maintenance that operates continuously in the animal body to oppose the effects of stress. The theory endeavors to fit all known facts, and is based on the hypothesis that coagulation Factors VII and VIII are respectively local and systemic stress agents that regulate thrombin activity and synergize each other's effects. Stress Theory may explain the etiologies of several hitherto mysterious disease syndromes, and the stress mechanism lo and may play a more pervasive role in disease than is generally appreciated. The theory offers fresh avenues for research and clinical strategy.
Owner:LEWIS S COLEMAN MD

Deep learning-based chromosome uploidy prediction system and method, terminal and medium

InactiveCN112990319AGood for euploidy prediction tasksExtraction is done efficientlyMedical data miningMedical automated diagnosisPattern recognitionData set
The invention belongs to the technical field of biomedicine, and discloses a deep learning-based chromosome uploidy prediction system and method, terminal and medium, and the method comprises the steps: selecting a part of embryo images in the whole process of embryo development, combining the images into a video as a training data set according to the time sequence, building a video sequence feature network model, training the embryo video, extracting morphological characteristics and dynamic characteristics in the embryo development process; and fusing the embryo video features and the clinical data features of the patient, and training a network according to the fused features to obtain a prediction result. According to the method, a video sequence feature network framework is creatively used, the space-time characteristics in the embryo development process are well obtained, and the extraction of the dynamic characteristics and the morphological characteristics of the embryo cells is effectively completed; the prediction model provided by the invention can automatically complete the prediction of the chromosome uploidy, and no manual intervention exists in the prediction process.
Owner:WUHAN MUTUAL UNITED TECH CO LTD

JY-1 Regulation Of Granulosa Cell Function And Early Embryonic Development In Cattle

The present invention provides compositions and methods for regulating fertility in mammals. In general, the invention relates to a novel protein produced by oocytes named JY-1, and nucleic acids encoding the JY-1 protein, for controlling folliculogenesis and early embryonic development, particularly in monoovulatory species. In particular, the present invention provides nucleic acid and amino acid sequences encoding JY-1, vectors for the expression of JY-1, host cells expressing JY-1, RNAi probes for reducing levels of JY-1 message, and antibodies to JY-1. Specifically, developing and mature oocytes express JY-1 in vivo, while granulosa cells treated in vitro with recombinant JY-1 (rJY-1) protein reduced cell proliferation while increasing progesterone synthesis and estradiol production. Further, reducing JY-1 protein in developing embryos in vitro using inhibitory siRNA constructs corresponded with arrested blastocyte maturation.
Owner:MICHIGAN STATE UNIV OFFICE OF INTPROP

Chemical toxicity detection method based on zebra fish behaviors

The invention provides a chemical toxicity detection method based on zebra fish behaviors. The method comprises the steps of exposing in a chemical solution, and carrying out embryonic development detection and fry behavior test on the zebra fish, thereby detecting the toxicity of the chemical. Compared with a molecular experiment, the method is simple in technology, easy to operate and free of special training; and meanwhile, the fishes have optical transparency, are convenient to observe, have large embryo spawning quantity and low cost, and have no special requirements on sites, so that theapplication threshold of the method is reduced. The rapid screening of the toxicity of chemicals is realized through a method of embryo development detection and larva fish behavior analysis, and themethod has a wide application prospect.
Owner:INNER MONGOLIA UNIVERSITY

Lowline embryo freezing liquid, freezing method and unfreezing method

The invention discloses a lowline embryo freezing liquid, a freezing method and an unfreezing method and belongs to the technical field of bioengineering and embryonic heredity. The lowline embryo freezing liqui comprises ethanediol, cane sugar and DMSO. The freezing method comprises the following steps: freezing an obtained embryo for pre-treating, putting the embryo in a tube and freezing the embryo. The unfreezing method comprises the following steps: retaining a straw in air for 5-10 seconds, putting the straw in water bath at the temperature of 35-38 DEG C for 10-15 seconds, detecting the embryo, transferring the embryo in WM1 liquid drops, transferring into another hole liquid drops containing the same solution, then respectively transferring to WM2 and liquid drop containing HM with the concentration being 800 microliter per hole and retaining for 5 minutes each time; and washing the embryo by a culture solution for three times, continuously observing the development condition of the embryo in the culture solution or transplanting the embryo to a receptor cow after being subjected to estrus synchronization. The freezing liquid, the freezing method and the unfreezing method have the good freezing effect, are strong in operability, convenient to operate and suitable for popularization and application.
Owner:南宁培元基因科技有限公司

Method for continuous observation of Tiretrack eel embryonic development

InactiveCN105123575AIncreased levels of developmental researchClear visionClimate change adaptationPisciculture and aquariaPetri dishPhysiology
The invention belongs to the technical field of aquatic animal breeding biology and discloses a method for continuous observation of Tiretrack eel embryonic development. A tiretrack eel oosperm is processed by trypsin solution and then continuous microscopic observation is conducted; the method further comprises steps of preparation of the trypsin solution, acquisition and processing of the tiretrack eel oosperm, continuous microscopic observation of the oosperm and daily management. The concentration of the trypsin solution is 1 to 3g / L; and 3 to 5ml is filled into each culture dish; after the tiretrack eel oosperm is processed by the trypsin solution, the external film of the oosperm is thinned and becomes transparent and then continuous microscopic observation can be achieved; clear view can be provided during the observation and reliable results can be achieved; compared with the same batches of oosperms yet to be processed by the trypsin solution, each development phase can be clearly observed during the embryonic development; and fish embryonic development research level is raised to a high new phase due to the formation of radial oosperm holes features of red artery and venous blood circulation system, so the method has huge research and reference value.
Owner:珠海市现代农业发展中心

Embryo pregnancy state intelligent prediction method and system

The invention discloses an embryo pregnancy state intelligent prediction method and system. The method comprises the steps of collecting embryo images within the time of D1 to D6; inputting the embryoimage into a fragment proportion prediction network model, a blastocyst cavity and inner cell mass grade prediction network model and a trophoblast grade prediction network model to calculate and outputting a predicted fragment proportion, a predicted blastocyst cavity proportion, a predicted inner cell mass grade and a predicted trophoblast grade of the embryo image; and inputting into an embryopregnancy rate state prediction machine learning model to calculate and output an embryo pregnancy rate prediction result. According to the intelligent prediction method and the system, the whole embryo development process is monitored, the embryo pregnancy rate is obtained through calculation by means of the comprehensive scoring function, manual intervention is not needed in the prediction process, and doctors can be helped to quickly and accurately judge embryo scores.
Owner:WUHAN MUTUAL UNITED TECH CO LTD

Modified milk powder made from yak milk for pregnant women and preparation method

The invention discloses modified milk powder made from yak milk for pregnant women. The modified milk powder is prepared from the following components in percentage by mass: 28-30% of yak milk powder, 21-23% of condensed whey protein powder, 19-20% of yak nonfat dry milk powder, 12-13% of compound vegetable oil, 8.5-9.5% of fructo-oligosaccharide, 7-8% of cane sugar, 0.3% of a vitamin premix, 0.82% of a mineral premix, and 25mg / 100g algae oil DHA and is prepared by adding nutrient substances of the condensed whey protein powder, the nonfat dry milk powder, the prebiotics, the compound vegetable oil and the like based on the yak milk powder. According to the formula disclosed by the invention, organic yak milk produced by naturally grazed yaks in natural pastures is used as a main raw material, remaining pollutants of antibiotics, plasticizers, pesticides, veterinary medicines and the like are not contained, and the prebiotics, the algae oil DHA, vitamins and minerals are added, so that sufficient and balanced nutrients can be well provided for the health of the pregnant women and prenatal development.
Owner:TIBET TREASURE OF PLATEAU YAK DAIRY IND +2

Detection and reorganization of hatching living bird embryo

InactiveCN101926290AAvicultureElectricityPattern recognition problem
The invention relates to detection and reorganization of a hatching living bird embryo. At present, testing methods of the hatching living bird embryo comprise apexcardiogram (ACG), ballistocardiogram (BCG), electrocardiograph (ECG) and impedance cardiogram (ICG). Because the methods have a variety of defects, a testing system of a weak acoustic signal of the living bird embryo is designed by using the weak acoustic signal sent by the hatching living bird embryo and combining the acoustic theory with the characteristics of a rotational ellipsoid, therefore, the invention solves the problem of recognizing the mode of the living bird embryo. Tests on the weak acoustic signal of the living chick embryo show that: the detection system has the advantages of novel design, simple operation, strong practicability, low cost and remarkable effect, and can not cause any adverse effects on the living chick embryonic development. The invention is also suitable for the mode reorganization and the weak acoustic signal detection of other living bird embryos.
Owner:QINGDAO TECHNOLOGICAL UNIVERSITY

Method for testing development process of silkworm embryos

The invention discloses a method for testing the development process of silkworm embryos. The method specifically comprises the following steps of: (1), establishing a model expressing the incidence relation between the relative transcription expression quantity of the acetylcholine esterase type 1 (ace1) of a to-be-tested variety of the silkworm embryos and the development process of the embryos; (2), selecting at least one embryo from the to-be-tested silkworm embryos to use as a sample, testing the relative transcription expression quantity of the ace1 of the sample, and then contrasting with the model obtained in step (1) to determine the development process of the silkworm embryos according to the relative transcription expression quantity of the ace1 of the to-be-tested sample. The development stage of a whole graine can be accurately determined by the method through the test on the development process of a small quantity of silkworm embryos. Manual operation errors can be reduced, the test process is simplified, and a development process identification method is provided for molecular biology study of the silkworm embryos.
Owner:SUZHOU UNIV

Zuogui pill serum containing active ingredients of Zuogui pill serum metabolic substance groups and preparation method and application of Zuogui pill serum

The invention discloses Zuogui pill serum containing active ingredients of Zuogui pill serum metabolic substance groups and a preparation method and application of the Zuogui pill serum. The preparation method includes the steps of (1), mixing medicinal materials for preparing Zuogui pills according to a formulation dosage for preparing the Zuogui pills and performing water extraction so as to obtain water extract; (2), administrating the water extract in animals at a dosage of 10-20 g kg<-1> d<-1> for 7-10 days continuously, once to twice per day, collecting blood after administration, and collecting serum after blood centrifugation so as to obtain the Zuogui pill serum. The Zuogui pill serum is highly rich in glycoside active ingredients and is capable of remarkably increasing an inhibited embryo development rate under intervention of high glucose to promote embryo development.
Owner:SHANXI UNIV OF CHINESE MEDICINE

Method for improving compatibility and development rate of interracial nuclear transfer embryo nucleoplasm

The invention provides a method for improving compatibility and development rate of interracial nuclear transfer embryo nucleoplasm. The method includes the steps: (a) a cell nucleus of a nuclear donor cell is moved into a perivitelline space of an enucleated oocyte of a non-human mammal and integrated by means of electric stimulation, so that a reconstructed ovum is formed, (b) a human oocyte is moved into the reconstructed ovum in the step (a), and (c) the reconstructed ovum in the step (b) is electrically stimulated so as to form a reconstructed ovum composed of the cell nucleus of a human cell, the enucleated oocyte of the non-human mammal and the human oocyte. The embryo development rate can be increased remarkably by the method.
Owner:SHANGHAI TRANSGENIC RES CENT

Method for efficiently preparing cloned embryos of sheep

The invention discloses a method for efficiently preparing high-quality cloned embryos, belonging to the field of embryo transplanting methods. The method provided by the invention comprises the following steps of: agitating and screening to obtain sheep oocytes in the same cell period and transplanting a supplier cell into the sheep oocytes through utilizing microscopy injection; then treating by utilizing a fusion solution and putting the treated embryo into a mature solution to be cultured to form a fused embryo; and chemically activating and then starting and reconstructing embryo cleavage. According to the method provided by the invention, the sheep oocytes which are in the same cell period and have the uniform mature time through an agitating method are acquired, a new method for picking the good sheep oocytes is provided and the mature rate of the sheep oocytes is improved. According to the method provided by the invention, the operation efficiency of nuclear transfer is improved by utilizing a method of simultaneously denucleating through extrusion and carrying out nucleus injection by utilizing the same injection needle. The fusion efficiency is improved to more than 95% by using the fusion solution which is developed by the method provided by the invention, the successful rate of the cloned embryo is greatly improved and the subsequent embryo development is not influenced. The method provided by the invention has the advantages of reducing the loss of the embryos in the process of preparing the cloned embryo and improving the efficiency of preparing the sheep cloned embryos; and the method provided by the invention is a method for efficiently preparing the sheep cloned embryos with good quality and has a wide application prospect.
Owner:INNER MONGOLIA AGRICULTURAL UNIVERSITY
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