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Method for continuous observation of Tiretrack eel embryonic development

An embryonic development and sustainable technology, applied in fish farming, application, climate change adaptation, etc., can solve problems such as unfavorable large spiny loach, affecting the progress of reproductive biology research of large spiny loach, fertilized egg shell thickness, etc. The effect of reliable results, clear characteristics, huge research and reference value

Inactive Publication Date: 2015-12-09
珠海市现代农业发展中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although there have been researches on the observation of the embryonic development of Echinococcus grandis, due to the thick eggshell of fertilized eggs, it is generally limited to regular microscopic photography and then processed with Photoshop software, and continuous microscopic observation has not been achieved, making it difficult to obtain a clear picture. The image of the embryonic development in order to ensure the correct observation results, which will greatly affect the research progress of the reproductive biology of the big cricket, which is not conducive to the large-scale artificial reproduction technology research of the big cricket

Method used

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  • Method for continuous observation of Tiretrack eel embryonic development
  • Method for continuous observation of Tiretrack eel embryonic development
  • Method for continuous observation of Tiretrack eel embryonic development

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Follow the steps below to observe the process of the embryonic development of the big eelfish:

[0023] 1 Preparation of experimental conditions

[0024] Experimental materials include fertilized eggs, trypsin, distilled water, petri dishes, etc., which were obtained by artificially induced production of the parents of Echinococcus grandis, as well as a set of binocular stereo microscopes, computers, and camera devices that can be used for microphotography; the experimental operation and observation process All are carried out in a constant temperature laboratory, and the indoor temperature is controlled at 26±0.5°C;

[0025] 2 Preparation of trypsin solution

[0026] Weigh 0.2g of trypsin (specific activity: 250U / mg) into a container bottle, add 100mL of distilled water, cover it and shake it constantly, let the trypsin fully dissolve, make a 2g / L trypsin solution, and place it in a constant temperature experiment room for use;

[0027] Obtaining and handling of fer...

Embodiment 2

[0038] Follow the steps below to observe the process of the embryonic development of the big eelfish:

[0039] (1) Preparation of trypsin solution: Weigh 0.1g of trypsin (specific activity: 200U / mg) into a container bottle, add 100mL of distilled water, cover it and shake it constantly, let the trypsin fully dissolve, and make 1g / L Trypsin solution, placed in a constant temperature laboratory for use;

[0040] (2) Acquisition and treatment of fertilized eggs of Echinococcus grandis: The fertilized eggs were obtained from the parents of Echinococcus grandis artificially, and the fertilized eggs were evenly distributed in the petri dish. The number of fertilized eggs in the petri dish was controlled at about 30. Add the trypsin solution to the eggs within 3 minutes, then add 3 mL of trypsin solution to each dish, shake the dish gently every 1 minute for 5-10 seconds, when 1 / 2 of the fertilized eggs in the dish begin to become clear , that is, pour out the trypsin solution, and ...

Embodiment 3

[0043] Follow the steps below to observe the process of the embryonic development of the big eelfish:

[0044](1) Preparation of trypsin solution: Weigh 0.3g of trypsin (specific activity: 300U / mg) into a container bottle, add 100mL of distilled water, cap it and shake it constantly, let the trypsin fully dissolve, and prepare 3g / L Trypsin solution, placed in a constant temperature laboratory for use;

[0045] (2) Acquisition and treatment of fertilized eggs of Echinococcus grandis: The fertilized eggs were obtained from the parents of Echinococcus grandis artificially, and the fertilized eggs were evenly distributed in the petri dish. The number of fertilized eggs in the petri dish was controlled at about 30. Add the trypsin solution to the eggs within 3 minutes, then add 5 mL of trypsin solution to each dish, shake the dish gently every 1 minute for 5-10 seconds, when about 1 / 3 of the fertilized eggs in the dish begin to become Translucent, that is, pour out the trypsin sol...

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Abstract

The invention belongs to the technical field of aquatic animal breeding biology and discloses a method for continuous observation of Tiretrack eel embryonic development. A tiretrack eel oosperm is processed by trypsin solution and then continuous microscopic observation is conducted; the method further comprises steps of preparation of the trypsin solution, acquisition and processing of the tiretrack eel oosperm, continuous microscopic observation of the oosperm and daily management. The concentration of the trypsin solution is 1 to 3g / L; and 3 to 5ml is filled into each culture dish; after the tiretrack eel oosperm is processed by the trypsin solution, the external film of the oosperm is thinned and becomes transparent and then continuous microscopic observation can be achieved; clear view can be provided during the observation and reliable results can be achieved; compared with the same batches of oosperms yet to be processed by the trypsin solution, each development phase can be clearly observed during the embryonic development; and fish embryonic development research level is raised to a high new phase due to the formation of radial oosperm holes features of red artery and venous blood circulation system, so the method has huge research and reference value.

Description

technical field [0001] The invention belongs to the technical field of reproductive biology of aquatic animals, and in particular relates to a method for sustainably observing the embryonic development of echinococcidae. Background technique [0002] Mastacembelus armatus belongs to Perciformes, Mastacembelus suborder, Mastacembelus armatus, Mastacembelus genus. Commonly known as Nacone, Stone Cone, Hemp Cut, Chili Fish, Swordfish. The body length of adult fish is 30-50 cm, the weight is 200-500 grams, and the largest individual can reach 2.5 kg. The meat is thin and firm, sweet and delicious, and rich in nutrition. It is mainly distributed in various water systems south of the Yangtze River, and the wild resources in the rivers of Guangdong, Guangxi, Fujian and other places are particularly rich. After the 1980s, due to excessive fishing and environmental pollution of the river, the wild resources of the rivers and rivers of the echinacea were severely damaged and the res...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCA01K61/00Y02A40/81
Inventor 骆明飞林义浩
Owner 珠海市现代农业发展中心
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