45 results about "Reproductive biology" patented technology

The invention discloses a technology for ecological breeding of large Chinese mitten crabs (female crabs 150g and male crabs more than 200g) in a pond. The method comprises the steps of (1) selection and transportation of the large parent crabs, (2) mating of the parent crabs, (3) over-wintering fatting and management of the parent crabs, (4) catching and temporary breeding of egg-carrying crabs, (5) cage hanging and distribution of the egg-carrying crabs, (6) food organism culture, (7) zoea cultivation, (8) megalopa desalting. The technology for ecological breeding of the large Chinese mitten crabs in the pond is developed for emphasizing to improve the over-wintering survival rate and the egg-carrying rate of the large parent crabs and the survival rate of the egg-carrying crabs according to features of reproductive biology and reproductive ecology of the Chinese mitten crabs on a basis of in-pond ecological breeding work of large parent crabs. Technical practices of an inventor for nearly a year proves that unprecedented success on the in-pond ecological breeding of the large parent crabs is achieved, an in-pond ecological breeding technical system of the large parent crabs is established, and profound influence is made in the Chinese mitten crab breeding industry of our country.

The invention provides a reinforced cultivating method of freshwater shrimp seedshrimp, which is a reinforced scientific and reasonable cultivating method of freshwater shrimp seedshrimp with low investment and high efficiency in scales through the technologies of selecting seedshrimp in cultivating ponds, building a pond environment, optimizing and regulating water environment, feeding and managing scientifically and the like according to freshwater shrimp aquaculture biology and reproductive biology. The seedshrimp cultivated by the method in the invention can produce shrimp seedlings by over 20% compared with the seedshrimp cultivated by conventional methods under the same seedling conditions. The method in the invention can be used for the large-scale shrimp seedling production of high-quality fresh water shrimp, guarantee smooth promotion of high-quality fresh water shrimp, improve economic efficiency of fresh water cultivation, and improve healthy and sustainable development of fresh water shrimp cultivating industry in China.

The present invention relates to compositions and methods for the handling of processed sperm including samples that are freshly collected, those transported as fresh samples, samples that are frozen and thawed, those sorted into one or more subpopulations, and those that are otherwise processed or handled that impose trauma on the cell. Trauma can reduce the motility, fertility, viability and overall integrity of the sperm and reduce the ability to fertilize, produce an embryo and a healthy offspring. The present invention relates to novel compounds that can be added to the sperm cell sample to reduce the traumatic effects of physical stress during mild as well as extensive sperm cell processing, methods of using the compounds in standard sperm processing procedures, the end products made from these methods including sperm and embryos, as well as methods of using those end products in assisted reproductive biology techniques in animals.

The invention discloses a method for establishing a bastard halibut brain tissue-derived cell system. The method comprises brain tissue primary culture, subculture, cryopreservation, recovery and identification, wherein cell culture fluid adopted is obtained by adding fetal calf serum and human basic fibroblast growth factor to basal cell culture fluid. The established bastard halibut brain cell system is represented as an epithelioid-like astrocytic cell, which can support continuous passage to provide a great amount of bastard halibut brain cells and can be directly used for research of bastard halibut functional gene. The construction method disclosed by the invention not only is expected to serve as a platform for theoretical research of bastard halibut molecule cellular level but also can be used as an ideal material for research on fish hemadenology, reproductive biology and environmental internal-secretion interfering-substance toxicology.

The invention relates to an artificial breeding method for tuba false fusus, which comprises the following steps of: on the basis of investigating tuba false fusus resources along the coast of Hainan and reproductive biology, finding out the thriving propagation season of the tuba false fusus, and catching parent tuba false fusus in the season; putting healthy adult tuba false fusus into a nursery pond for intensified breeding, and optimizing breeding conditions such as water flow, illumination, water temperature, and salinity; after the tuba false fusus is mated and lays eggs, cutting off an egg capsule, and transferring to an incubation basket for flowing water breeding; after young tuba false fusus is hatched, transferring to a breeding basket, feeding concentrated chlamydomonas in first 4 to 6 days, and then feeding fresh clam and Sinonovacula constricta; when the young tuba false fusus grows to 2.0-2.5cm, dividing the young tuba false fusus; and when the young uba false fusus grows to about 4.0cm in the breeding basket, entering a culture stage. Based on the reproductive biological process of the tuba false fusus, the method realizes indoor artificial breeding of the tuba false fusus, shortens the artificial breeding cycle, is suitable for mass production, and has the advantages of simpleness, practicability, convenient operation, low production cost, and high survival rate of the bred tuba false fusus.

The invention provides a scale estimation method for a spawning ground of river fishes capable of laying adhesive eggs. According to the scale estimation method, a spawning ground scale T is estimated according to an investigation object population total number S, a sexual maturity individual number ratio P1, a reproductive population female number ratio P2, a after-egg laying individual ratio P3 and a relative fish brood amount R; and the spawning ground scale T is equal to S*P1*P2*P3*w*R. According to the method provided by the invention, fishing investigation is combined with fish population growth and death parameter estimation, and reproductive biology parameters; and the scale estimation problem of the spawning ground of the river fishes capable of laying the adhesive eggs is solved, so that the difficulties that area of an egg laying region and fish egg density and the like underwater are difficult to count, and equipment cannot develop the work while the equipment does not meet the requirements are avoided.

The present invention relates to a new biomarker for the medicine and the biology of reproduction, in particular for in vitro fertilization (IVF) outcome. It relates to methods for predicting IVF outcome and for selecting the subject for IVF.

The invention discloses a hybrid breeding method of lutmria maxima jonas and Lutraria philippinarum. The lutmria maxima jonas grows rapidly and is high in price, but cultivation is affected by bottom tide, storm and diseases, and annual fluctuation of the output is large. The breeding mode for Lutraria philippinarum is simpleand economic effect is obvious, but the disease resistance of the young Lutraria philippinarum is poor, and it is difficult to artificially breed. The method comprises the steps of: carrying out interspecific hybridization according to the situation that lutmria maxima jonas and Lutraria philippinarum are geographically isolated and different in reproductive biology characteristics, and breeding filial generation with merits such as strong disease resistance and fast growth speed. According to the invention, the measures such as purchasing and transporting of parent shellfish, domesticating of the parent shellfish, artificial fertilization and hybridization, incubation and superior varity selection, cultivation of planktonic larvae, larval metamorphosis, juvenile mollusk cultivation and intermediate cultivation in a sear area are adopted. The hybrid breeding method is scientific and reasonable in technological process and strong in maneuverability, and hybridization of two types of geographically isolated clams belonging to Lutraria is achieved. The method disclosed by the invention is fewer in occupied resources and low in cost, and natural resources are fully utilized.

The present invention relates to a novel propagation technology which is based on the principle of heterogeneous livestock semen assistant function, so as to produce the low-dose target livestock semen and use the product to implement the in vivo / in vitro fertilization. The main contents include producing the low-dose target livestock fresh and frozen semen by adding heterogeneous livestock semen and using the target livestock fresh and frozen semen produced by adding heterogeneous livestock semen to carry out the in vitro fertilization and artificial fertilization. The invention can use a small amount of semen of the target livestock (1 to 50 percent of the normally required concentration) to reach the normal fertilization and pregnant level through the assistant function of the heterogeneous livestock semen. The technology can greatly improve the utilization rate of the target livestock, which is used for breeding and propagation of the target livestock, as well as the infertility caused by poor semen quality indicators of rare semen and poor vitality etc. of special target male livestock. In addition, the technology can also be taken as a novel method of the study of reproductive biology.

The invention relates to a screening method of advantage-based stock seed of isatis root, and is applicable to improved variety breeding of isatis root. The screening method comprises the steps of: collecting isatis root germ plasma resources and building a resource garden; evaluating individuals and small groups of different germ plasma species according to the individual yield, inherent quality, commercial product characters and reproductive biological characteristics, carrying out germ plasma breeding and screening for continuous two years to obtain advantage-based stock seed of isatis root. The obtained advantage-based stock seed has the advantages that the principal root is long and thick, the leaves are long and narrow, the leaves are dark green, the individual has multiple leaves, and the dry weights of the leaves and root of the individual are high; the seeds are long and wide, full in grains and high in weight of thousand grains; after being conventionally sundried, the isatis root has adenosine content of 6.0-7.5% and alcohol extract content of 25-33%; and isatis leaf has indirubin content of 0.05-0.06% and alcohol extract content of 28-30%.

The present invention generally relates to compositions and methods for the handling of processed sperm populations including samples that are freshly collected, those transported as fresh samples, as well as samples that are frozen and thawed, those sorted into one or more subpopulations, and those that are otherwise processed or handled that impose trauma on the sperm cell. Such trauma can reduce the motility, fertility, viability and overall integrity of the sperm and reduce the sperm's ability to fertilize an egg, grow into a health embryo and produce a healthy offspring. The present invention relates to novel compounds that can be added to the sperm cell sample to reduce the traumatic effects of physical stress during mild as well as extensive sperm cell processing, methods of using the compounds in standard sperm processing procedures, the end products made from these methods including sperm and embryos, as well as methods of using those end products in assisted reproductive biology techniques in animals

The invention discloses a novel preparation method of yolk diluent, belonging to the field of animal reproductive biology. The novel preparation method comprises the following steps of: preparing yolk: preparing 4.6 times of Tris-A mother solution and 6 times of Tris-B mother solution and carrying out the aseptic filtration; diluting into 0.5 time of Tris-A mother solution and Tris-B mother solution with sterile water; respectively mixing with the yolk, stirring, standing, centrifuging and obtaining supernatant; respectively adjusting the concentration of the supernatant with 4.6 times of Tris-A mother solution and 6 times of Tris-B mother solution to obtain 1 time of Tris-A mother solution and Tris-B mother solution; and respectively adding antibiotic and sterile glycerin, adjusting the pH value and storing the mixture at 4DEG C for later use. The method omits the filtering step and is simple; fewer granules exist in the yolk diluent; and the method ensures the motility rate of cryopreservation sperms, improves the quality of froze sperm products and reduces the production cost.

The invention discloses a suction and rotary scraping type endometrial epithelium cell gathering device for goat and belongs to the technical field of reproductive biology. The suction and rotary scraping type endometrial epithelium cell gathering device for goat is composed of a gathering head (54), a gathering body (55), a connecting barrel (20), a power part (56), a handle (32), a power supply plug (26) and a power cord (27) and characterized in that a screen (1) forms the pear-shaped part of the gathering head (54), when using, the gathering head (54) clings to the endometrium, a scraper (2) is composed of a rotary cutter (33), a cutter swinging handle (34), a swinging handle fixing disc (35), a swinging handle fixing disc shaft (36), a telescopic handle (37) and a telescopic handle shaft (38); the disc-shaped rotary cutter (33) comprises four rotary cutter knives (43) which are vertical to each other, the rotary cutter knives are double prism-shaped, and all the edges have blades. The suction and rotary scraping type endometrial epithelium cell gathering device for goat is easy to manufacture, strong in maneuverability, low in cost and obvious in effect.

The present invention relates to compositions and methods for the handling of processed sperm including samples that are freshly collected, those transported as fresh samples, samples that are frozen and thawed, those sorted into one or more subpopulations, and those that are otherwise processed or handled that impose trauma on the cell. Trauma can reduce the motility, fertility, viability and overall integrity of the sperm and reduce the ability to fertilize, produce an embryo and a healthy offspring. The present invention relates to novel compounds that can be added to the sperm cell sample to reduce the traumatic effects of physical stress during mild as well as extensive sperm cell processing, methods of using the compounds in standard sperm processing procedures, the end products made from these methods including sperm and embryos, as well as methods of using those end products in assisted reproductive biology techniques in animals.

The invention relates to a method for obtaining cloned pigs with modified genes through continuous cloning and belongs to the field of animal reproductive biology.According to the method, tissue fibroblasts of obtained genetically modified pigs serve as donors, donor cells are transferred into enucleated porcine oocytes through the somatic cell nuclear transfer to build a genetically modified pig reconstructed embryos, then electro-fusion and electro-activation are conducted, the donor cells are transferred into estrous surrogate sow fallopian tube expanded portions after being cultured in a pig embryo in-vitro culture solution for 3 h, and after gestation of surrogate sows last for 114 d, a large scale of genetically modified pig individuals are obtained through delivery.The method can solve the problems that in the process that genetically modified individuals are obtained through the gene editing technology, target genetically modified individuals are low in efficiency, and difference between the target genetically modified individuals is large, so that genetically modified cloned pig individuals stable in target gene expression and phenotype can be efficiently produced on a large scale.

The invention relates to a non-animal origin tree shrew sperm in vitro capacitation liquid and application thereof, belonging to the technical field of animal reproductive biology. The specific stepsare as follows: preparing tree shrew ishing semen and capacitation liquid; separating and removing the epididymis cauda of tree shrews; placing the epididymis cauda in centrifugal tubes containing ished semen and incubating for 10 min to allow sperm to swim out. transferring the sperm suspension to the sterilized centrifuge tube, adding the semen ishing liquid, gently mixing, and centrifuging; discarding supernatant, adding semen ishing liquid, and centrifuging; discardingthe supernatant and resuspending the sperm in the capacitation solution to make the sperm concentration 10* 106 sperm / mL.5 ml of that semen is divided into incubation tubes and incubating in a CO2 incubator. A method for in vitro capacitation of tree shrew spermatozoa adopting animal-free component capacitation liquid avoids that risk of introduce pathogenic factors into in vitro capacitation or in vitro fertilization system, and is easy to carry out quality control on the capacitation liquid.

The invention discloses a method for promoting aseptic germination of Dendrobium officinale and a culture medium used therein, and the application of the method. According to the invention, through research on phylembryogenesis and fruit maturity of the Dendrobium officinale, the aseptic germination conditions of the Dendrobium officinale are selected; a rapid propagation system is optimized; theproper seed age of sowing for aseptic germination of the Dendrobium officinale is determined; and Dendrobium officinale seeds with a proper seed age are sown into an improved solid culture medium to obtain protocorms with high germination rate and neat uniform germination state. The method provided by the invention enriches the reproductive biology development resources of the Dendrobium officinale and provides a theoretical basis for aseptic germination; through selection of the Dendrobium officinale seeds with a proper seed age as explants for tissue-culture rapid propagation, the method provided by the invention greatly shortens the propagation and breeding time of the Dendrobium officinale seeds and has more advantages in scale and industrialization development of the Dendrobium officinale. The protocorms obtained by culturing in the improved solid culture medium have high germination rate and neat and uniform germination state, and provide resources for the protection of wild resources through establishment of an aseptic germination system of the Dendrobium officinale and obtainment of excellent seedlings in production.

The invention relates to a method for producing a parthenogenetic cloned pig by means of continuous cloning technology, which belongs to the field of reproductive biology. The method provided by the invention comprises the following steps of: the degreasing of a porcine oocyte, parthenogenetic activation, the separation of a fibroblast, and continuous somatic nuclear transfer; and specifically, the method comprises the following steps of: carrying out the parthenogenetic activation on the degreased mature porcine oocyte to obtain a parthenogenetic embryo, transplanting the parthenogenetic embryo into the body of a surrogate sow to obtain a 25-29 days old living parthenogenetic fetus, establishing a porcine parthenogenetic fetus fibroblast line which is used as a preliminary donor cell, and carrying out continuous cloning by means of somatic cell nuclear transfer technique to obtain a porcine parthenogenetic individual. By adopting the method provided by the invention, the condition that the in-vivo development stops when the porcine parthenogenetic fetus grows for 30 days is overcome, the in-vivo development time of the porcine parthenogenetic fetus is successfully prolonged, and therefore a living parthenogenetic cloned pig can be successfully obtained finally.

The present invention relates to a novel propagation technology which is based on the principle of heterogeneous livestock semen assistant function, so as to produce the low-dose target livestock semen and use the product to implement the in vivo / in vitro fertilization. The main contents include producing the low-dose target livestock fresh and frozen semen by adding heterogeneous livestock semen and using the target livestock fresh and frozen semen produced by adding heterogeneous livestock semen to carry out the in vitro fertilization and artificial fertilization. The invention can use a small amount of semen of the target livestock (1 to 50 percent of the normally required concentration) to reach the normal fertilization and pregnant level through the assistant function of the heterogeneous livestock semen. The technology can greatly improve the utilization rate of the target livestock, which is used for breeding and propagation of the target livestock, as well as the infertility caused by poor semen quality indicators of rare semen and poor vitality etc. of special target male livestock. In addition, the technology can also be taken as a novel method of the study of reproductive biology.

The invention discloses an artificial fry rearing method for gadus macrocephalus. In the industry, researches on the gadus macrocephalus with significant economic values are still in blank, and researches on reproductive biology, artificial breeding, proliferation and the like of the gadus macrocephalus are not developed yet. The artificial fry rearing method for the gadus macrocephalus has the advantages that fries of gadus macrocephalus can be cultivated to adult fish successfully, fish fry quality can be improved, survival rate can be guaranteed, industrialized production can be realized smoothly, and theoretical foundation and technical support are provided for large-scale aquaculture of the gadus macrocephalus.

The invention discloses a systematic evaluation method of lily resource powder characteristics and application thereof, and relates to the research field of lily reproductive biology and crossbreeding. The method comprises the following steps: (1) observing and counting single flower anther number of the lily resource; (2) obtaining anther of a sample to be measured and observing whether anther dehiscence happens; (3) measuring length, width and thickness of the anther and calculating anther size; (4) measuring pollen content of the anther and pollen content of a single flower; (5) calculating pollen content of the anther per unit volume according to the pollen content of the anther and anther size; and (6) determining apparent pollen quantity and pollen pollution to petals during the lily source powder growing period. A systematic evaluation method of lily resource powder characteristics is established in the invention. The invention lays an importation foundation for evaluation of lily resource powder characteristics, the research on reproductive development mechanism of powder quantity difference and breeding of pollen pollution-free lily new-variety.

The invention discloses a method for breeding a Magnolia grandiflora-magnolia liliiflora hybrid. According to the method, at first, magnolia liliiflora pollen as a male parent obtained in the magnolia liliiflora flowering phase earlier two months than the Magnolia grandiflora flowering phase is preserved and maintains pollen activity by collecting initially open magnolia liliiflora flowers, taking out stamens, uniformly spreading the stamens on a vegetable parchment, naturally drying at a room temperature, after anthers of the stamens crack and pollen is spilled out, collecting the pollen by an iron screen of 80 meshes, drying it in a dry silicon dish by a filter paper bag, closely wrapping the dried pollen by a silver paper, preserving the wrapped pollen under the freezer condition of a temperature of -70 DEG C, and before use, taking out the pollen and carrying out stepwise heating thawing. The method also comprises determination of the best pollination period of Magnolia grandiflora as a female parent, cross pollination reproductive biology basic research, hybrid fruit and hybrid development situation observation and breeding and preservation methods. The method for hybridization can be operated easily and can effectively produce Magnolia grandiflora-magnolia liliiflora hybrid seeds.

The invention provides a scale estimation method for a spawning ground of river fishes capable of laying adhesive eggs. According to the scale estimation method, a spawning ground scale T is estimated according to an investigation object population total number S, a sexual maturity individual number ratio P1, a reproductive population female number ratio P2, a after-egg laying individual ratio P3 and a relative fish brood amount R; and the spawning ground scale T is equal to S*P1*P2*P3*w*R. According to the method provided by the invention, fishing investigation is combined with fish population growth and death parameter estimation, and reproductive biology parameters; and the scale estimation problem of the spawning ground of the river fishes capable of laying the adhesive eggs is solved, so that the difficulties that area of an egg laying region and fish egg density and the like underwater are difficult to count, and equipment cannot develop the work while the equipment does not meet the requirements are avoided.

The present invention generally relates to compositions and methods for the handling of processed sperm populations including samples that are freshly collected, those transported as fresh samples, as well as samples that are frozen and thawed, those sorted into one or more subpopulations, and those that are otherwise processed or handled that impose trauma on the sperm cell. Such trauma can reduce the motility, fertility, viability and overall integrity of the sperm and reduce the sperm's ability to fertilize an egg, grow into a health embryo and produce a healthy offspring. The present invention relates to novel compounds that can be added to the sperm cell sample to reduce the traumatic effects of physical stress during mild as well as extensive sperm cell processing, methods of using the compounds in standard sperm processing procedures, the end products made from these methods including sperm and embryos, as well as methods of using those end products in assisted reproductive biology techniques in animals.

The invention discloses a culture solution, and a method for improving the quality of maternal diabetic ova, and relates to the technical field of reproductive biology. The culture solution is used for in-vitro culture of maternal diabetic cumulus-oocyte complexes, the culture solution comprises tea polyphenols, in-vitro culture is performed in the culture solution containing the tea polyphenols, and the quality of the ova is effectively improved. The tea polyphenol used in the culture is from a tea leaf extract, belongs to a natural active substance, has no toxic or side effect on human bodies, animals and the like, is good in safety, and is easy to obtain and low in cost. The culture solution provided by the invention can improve the quality of maternal diabetic ova and improve the fertility problem of maternal diabetic patients.

The invention relates to a screening method of advantage-based stock seed of isatis root, and is applicable to improved variety breeding of isatis root. The screening method comprises the steps of: collecting isatis root germ plasma resources and building a resource garden; evaluating individuals and small groups of different germ plasma species according to the individual yield, inherent quality, commercial product characters and reproductive biological characteristics, carrying out germ plasma breeding and screening for continuous two years to obtain advantage-based stock seed of isatis root. The obtained advantage-based stock seed has the advantages that the principal root is long and thick, the leaves are long and narrow, the leaves are dark green, the individual has multiple leaves, and the dry weights of the leaves and root of the individual are high; the seeds are long and wide, full in grains and high in weight of thousand grains; after being conventionally sundried, the isatis root has adenosine content of 6.0-7.5% and alcohol extract content of 25-33%; and isatis leaf has indirubin content of 0.05-0.06% and alcohol extract content of 28-30%.

The present disclosure provides a combination of antisense RNA sequences and use thereof in the production of abortive tilapia, belonging to the technical field of molecular biology and reproductive biology, the combination of antisense RNA sequences includes antisense RNA of steroidgenic factors SF1-1 and SF1-2; the nucleotide sequences of Anti-SF1-1-I, Anti-SF1-1-II, Anti-SF1-2-I and Anti-SF1-2-II are set forth in SEQ ID NO. 1-SEQ ID NO. 4 respectively. The method of the present disclosure introduces antisense RNA fragments into the eggs through the fertilization hole to realize effective and accurate targeted intervention for regulating the gene expression, and the method has the advantages of simple operation, minimal egg damage, high success rate, stable phenotype after breeding, and excellent application prospects.

The invention provides a culture method for development of waste embryo blastocysts, and belongs to the cross field of microfluidic technology and reproductive biology. A chip used in the culture method is an ''inverted pyramid-shaped'' or ''inverted V-shaped'' chip with a concave array structure, and the surface of the chip is coated with Pluronic F-127. The embryo culture microfluidic chip is beneficial to realizing a single embryo positioning process and a culture process in vitro. According to the embryo culture chip and the culture method, the blastocyst development rate of waste embryoscan be obviously improved, and the embryo culture chip and the culture method have important significance for establishing human embryonic stem cell (hES) libraries of different human HLA types.