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Method for promoting aseptic germination of Dendrobium officinale and culture medium used therein, and application of method

A germination medium and technology of Dendrobium officinale, applied in the biological field, can solve problems such as unfavorable large-scale production, low seed germination rate, difficult pretreatment, etc., and achieve the effects of easy sowing operation, improved germination rate, and neat and consistent germination state.

Inactive Publication Date: 2019-03-05
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Orchids have a large number of seeds, but the germination rate of mature and cracked fruits is low and it is difficult to pretreat, and the infection rate after sowing is high, which is not conducive to the needs of large-scale production

Method used

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  • Method for promoting aseptic germination of Dendrobium officinale and culture medium used therein, and application of method
  • Method for promoting aseptic germination of Dendrobium officinale and culture medium used therein, and application of method
  • Method for promoting aseptic germination of Dendrobium officinale and culture medium used therein, and application of method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Embodiment 1, research on the embryonic development of Dendrobium candidum and the influence of different factors on the aseptic germination of Dendrobium candidum

[0081] (1) Embryo development of Dendrobium officinale

[0082] 1. Test materials

[0083] The test materials were collected in the greenhouse of the Institute of Flower and Botany, Guangxi Academy of Agricultural Sciences. On May 22, 2014, robust Dendrobium officinale plants were selected for artificial pollination, and the fruits were collected at intervals of 5 days for the test.

[0084] 2. Test method

[0085] 1. Stereo microscope morphology observation

[0086] After collecting the fruit, cross-cut the fruit with a double-sided blade, observe the morphological characteristics and development status of the fruit and seeds 0-180 days after pollination under a stereomicroscope (Leica DFC500), and select periods with typical morphological characteristics to take pictures. Morphological characteristics of...

Embodiment 2

[0172] Embodiment 2, dendrobium officinale suspension culture production polysaccharide

[0173] 1. Protocorm material for solid culture

[0174] 1. The test materials were collected in the Flower Plant Research Institute of Guangxi Academy of Agricultural Sciences. On May 22, 2014, robust Dendrobium officinale plants were selected for artificial pollination, and the fruits of 120DAP, 150DAP and 180DAP were collected, and 3 fruits were collected each time for the test.

[0175] 2. Seed pretreatment

[0176] Uncracked fruits were removed from the surface, and the surface of the fruit was cleaned with a toothbrush. The fruit was placed in a beaker and rinsed with running water for 1 hour, and then transferred to an aseptic operating table for sterilization. The sterilization procedure is: 75% alcohol for 30 seconds; soaking in 1% sodium hypochlorite solution for 15 minutes; washing with sterile water for 5 times, each time for 2 minutes. The sterilized fruit is taken out, blot...

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Abstract

The invention discloses a method for promoting aseptic germination of Dendrobium officinale and a culture medium used therein, and the application of the method. According to the invention, through research on phylembryogenesis and fruit maturity of the Dendrobium officinale, the aseptic germination conditions of the Dendrobium officinale are selected; a rapid propagation system is optimized; theproper seed age of sowing for aseptic germination of the Dendrobium officinale is determined; and Dendrobium officinale seeds with a proper seed age are sown into an improved solid culture medium to obtain protocorms with high germination rate and neat uniform germination state. The method provided by the invention enriches the reproductive biology development resources of the Dendrobium officinale and provides a theoretical basis for aseptic germination; through selection of the Dendrobium officinale seeds with a proper seed age as explants for tissue-culture rapid propagation, the method provided by the invention greatly shortens the propagation and breeding time of the Dendrobium officinale seeds and has more advantages in scale and industrialization development of the Dendrobium officinale. The protocorms obtained by culturing in the improved solid culture medium have high germination rate and neat and uniform germination state, and provide resources for the protection of wild resources through establishment of an aseptic germination system of the Dendrobium officinale and obtainment of excellent seedlings in production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for promoting the aseptic germination of Dendrobium officinale, a culture medium for it and application thereof. Background technique [0002] Dendrobium belongs to Orchidaceae, and most of the plants in the genus have important medicinal and ornamental value. Dendrobium contains about 1000 species of plants, mainly distributed in tropical and subtropical regions of Asia. In my country, Dendrobium plants are concentrated in the provinces and regions south of the Qinling Mountains, especially in the southern part of Yunnan Province (Chen Xinqi, 1999), of which there are about 50 species. species can be used medicinally (Baiyin, 2007). The 2010 edition of the Pharmacopoeia of the People's Republic of China includes five kinds of medicinal Dendrobium: Dendrobium loddigesii, Dendrobium fimbriatum, Dendrobium chrysanthum, Dendrobium nobile and Dendrobium officinale. ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 程瑾辛小雪刘岩沐先运刘忠华马文柯
Owner BEIJING FORESTRY UNIVERSITY
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