The invention relates to an animal model, and discloses an HBV (Hepatitis B Virus) infected tree shrew model construction method which specifically comprises the following steps: S1, respectively carrying out resuscitation, subculture and cryopreservation on cell strains; step S2, preparing an HBV (Hepatitis B Virus) culture solution; s3, carrying out HBV activity evaluation and screening the effective titer of an HBV culture solution; s4, obtaining a culture medium infected with HBV; step S5, screening out effective HBV supernate concentration; s6, setting experiment groups; and S7, constructing an animal model and obtaining an experimental conclusion according to experimental data. According to the invention, by constructing the HBV infected tree shrew model, the problems of relative hysteresis of HBV persistent infection, pathogenic mechanism research and development and evaluation of antiviral drugs due to lack of a stable and effective HBV animal model and the like can be effectively solved, and the HBV infected tree shrew model has great significance in further research of hepatitis B, hepatic fibrosis, liver cirrhosis, liver cancer and other diseases.