1298 results about "Cell model" patented technology

The present invention relates to a method and an apparatus for estimating discharge and charge power of battery applications, including battery packs used in Hybrid Electric Vehicles (HEV) and Electric Vehicles (EV). One charge / discharge power estimating method incorporates voltage, state-of-charge (SOC), power, and current design constraints and works for a user-specified prediction time horizon Δt. At least two cell models are used in calculating maximum charge / discharge power based on voltage limits. The first is a simple cell model that uses a Taylor-series expansion to linearize the equation involved. The second is a more complex and accurate model that models cell dynamics in discrete-time state-space form. The cell model can incorporate a inputs such as temperature, resistance, capacity, etc. One advantage of using model-based approach is that the same model may be used in both Kalman-filtering to produce the SOC and the estimation of maximum charge / discharge current based on voltage limits.

The present invention relates to a method and an apparatus for estimating discharge and charge power of battery applications, including battery packs used in Hybrid Electric Vehicles (HEV) and Electric Vehicles (EV). One charge / discharge power estimating method incorporates voltage, state-of-charge (SOC), power, and current design constraints and works for a user-specified prediction time horizon Δt. At least two cell models are used in calculating maximum charge / discharge power based on voltage limits. The first is a simple cell model that uses a Taylor-series expansion to linearize the equation involved. The second is a more complex and accurate model that models cell dynamics in discrete-time state-space form. The cell model can incorporate a inputs such as temperature, resistance, capacity, etc. One advantage of using model-based approach is that the same model may be used in both Kalman-filtering to produce the SOC and the estimation of maximum charge / discharge current based on voltage limits.

The invention discloses a delay and energy consumption-based efficient offloading method for a computing task of a mobile edge computing system. The method comprises the steps of building a macro basestation with multiple users and one deployment mobile edge computing (MEC) server and a single-cell cell model of a small base station without an MEC server; then building a mathematical model basedon a wireless communication theory; and finally, designing a delay and energy consumption-based efficient offloading solution for a computing task of the MEC system through a parallel greedy algorithm, and analyzing and providing the performance of the solution. Compared with existing efficient energy consumption offloading solution, the method well weights the delay sensitive demand and energy consumption demand of a terminal user, and lowers delay and total energy consumption of the system.

The invention relates to a method for preparing a chromosome translocation stem cell model and an animal model. The method mainly comprises the steps of inducing occurrence of specific site chromosome translocation in stem cells by means of CRISPR / Cas9 technology, preparing a cell model and further constructing an animal model carrying the specific site chromosome translocation by utilizing embryonic stem cell technology. The method particularly comprises the steps of simultaneously introducing Cas9 and gRNA aiming at two target chromosome sites into the stem cells to artificially inducing chromosome dislocation of specific sites, and screening to obtain the cell model carrying the chromosome translocation. The animal model carrying chromosome translation of the specific sites can be further prepared from the chromosome translocation animal embryonic stem cells carrying chromosome translation by utilizing a chimera technology. The method has relatively large application prospect in studies on chromosome translocation and functions of fusion genes, researches on chromosome interaction and medicine screening evaluation.

The invention discloses a complex braided structure ceramic matrix composite fatigue retardation behavior prediction method. The method comprises the steps of calculating a fatigue stress-strain curve under circulation with a microscale model to obtain the unit constitutive relation, substituting the unit constitutive relation into a unit cell model to conduct corresponding stiffness reduction on the unit cell model under different boundary conditions, and conducting statistics on the average stress and average strain of the unit cell model to obtain a unit cell scale fatigue retardation loop under the cycle number. By means of the multiscale model, the micro stress-strain field of a complex braided structure can be given, and therefore the fatigue retardation behavior of the material can be predicted precisely.

A system and a method for computing an estimated state of charge and an estimated cell resistance of an electrochemical cell are provided. The method includes predicting a first cell resistance value indicating a present resistance of the electrochemical cell utilizing a first nonlinear cell model. The method further includes predicting a first state of charge value indicating a present state of charge of the electrochemical cell utilizing a second nonlinear cell model. The method further includes measuring a voltage and, a current associated with the electrochemical cell to obtain a voltage value and a current value, respectively. The method further includes estimating a second state of charge value indicating the present state of charge of the electrochemical cell utilizing the second nonlinear cell model based on the first state of charge value, the first cell resistance value, the voltage value, and the current value. The method further includes estimating a second cell resistance value indicating the present resistance of the electrochemical cell utilizing the first nonlinear cell model based on the first cell resistance value, the first state of charge value, the voltage value, and the current value.

The invention discloses an in-vitro evaluation cell model of skin sensitization of a compound and a construction method of the cell model. The construction method of the cell model comprises the following steps of: designing and constructing an sgRNA (small guide ribonucleic acid) expression vector with CRISPR / Cas9 (clustered regularly interspaced short palindromic repeats / CRISPR associated protein 9), designing and constructing a homologous recombinant vector capable of knocking a reporter gene connected with a spontaneous lysis peptide sequence into an expression cassette of an HMOX1 (heme oxygenase (decycling) 1) gene, and cotransfecting a cell with the homologous recombinant vector, hCas9 (humanized Cas9) plasmid and the sgRNA expression vector for monoclone enlarging culture to form the cell model. The HaCaT cell model with a luciferase gene knocked in before a termination codon of the HMOX1 gene by a combined CRISPR / CAS9 cell monoclone technology. The cell model achieves synchronous expression of the luciferase gene and the HMOX1 gene, so that a sensitization compound and a non-sensitization compound are effectively differentiated, and the more specific and sensitive cell model is provided for compound sensitization studies.

The invention provides a construction method of a cell model for detecting pyrogens, the cell model and a pyrogen detection kit. The cell model utilizes specific locations of CRISPR / CAS9 induced genomes to form double-bond fission, TLR4 and CD14-MD2 are knocked into two chromosomes of a cell line respectively by the aid of the homologous recombination repair principle, green fluorescence GFP and red fluorescence RFP are respectively used for tracing finally successfully constructed TLR4 / CD14 / MD2 fixed-point knocked-in fluorescent tracer cell models, and the LPS stimulating cell model can detect release of IL-6 and TNF-a cytokines by means of ELISA, Western Blot, mass spectrum and immunomagnetic beads. The cell model is good in stability and high in sensitivity, and the lowest detectable limit can reach 0.005EU / mL and is far lower than 0.025EU / mL of the Tachypleus Amebocyte Lysate method.

The invention discloses a caco-2 cell model for CRISPR / CAS9-mediated drug transporter targeted knockout and a method thereof. The method is used for non-diagnostic or therapeutic purposes and comprises the following steps: designing sgRNA with target specificity for P-gp, BCRP and MRP2 transporters and constructing a sgRNA expression vector, wherein a sequence of the designed sgRNA is shown as SEQID NO. 1-6 in a sequence table; respectively designing P-gp, BCRP and MRP gene single knockout and pairwise combination double knockout by utilizing CRISPR / CAS9, co-transfecting a caco-2 cell with anhCas9 plasmid and performing monoclonal expansion culture to obtain the caco-2 cell model for transporter gene targeting. The caco-2 cell model obtained by the method disclosed by the invention has the beneficial effects that the mutual interference among different transporters is effectively eliminated, and a more specific and more sensitive cell model is provided for drug transport research.

The invention discloses a CRISPR / Cas9-gRNA targeting sequence pair, a plasmid and an HD model of HTT, and relates to the field of biotechnology. The targeting sequence pair of the invention comprisesan L sequence and an R sequence. The base sequence of the L sequence is shown as SEQ ID NO.1. The base sequence of the R sequence is shown as SEQ ID NO.2. The targeting plasmid of the invention comprises a first vector plasmid and the above-mentioned targeting sequence pair. The targeting sequence pair is constructed into the first vector plasmid. The HD cell model of the invention is obtained byco-transfecting cells with PolyQ Donor plasmids and the above-mentioned targeting plasmids. By using differentiated neuron cells as a carrier to construct the HD cell model, the invention provides a research platform for exploring the influences or changes of mHtt protein on the internal environment of differentiated nerve cells, and studying the changes of various signal pathways, metabolic pathways and intracellular homeostasis caused by mHtt protein in differentiated neuronal cells.

The invention belongs to the technical field of road design, and relates to a method for determining the minimum distance of road intersections in a harbor district. In the method, frequent lane-changing area of harbor district roads connected with the intersections and the intersections are subjected to two-dimensional cell division, and other parts are one-dimensional cells, and a cell model for harbor district roads near the intersections is constructed. The method comprises the following steps of: (1) constructing a driver sensing module; (2) constructing a behavior module; and (3) setting appearing ratios of various vehicles in a main road, speeds of the various vehicles, and departure frequencies of various vehicles, performing simulation experiments under the conditions of different distances of the intersections and different departure frequencies, and analyzing the simulation to obtain a traffic flow space-time map and vehicle delay data so as to obtain the minimum distance of proper intersections under different traffic conditions. By the method, the influence of the distance of the intersections on the road capacity of the harbor district can be accurately reflected, sothat a method for determining the distance of the road intersections in the harbor district under different conditions is provided.

The invention provides an antineoplastic drug screening cell model utilizing STAT3 as target, belonging to the field of biomedicine. The model is created by a reporting system carrier containing an STAT3 specific binding sequence on the basis of active cells with constitutive STAT3, and the model is used for screening treatment medicine of carcinomatous changes and tumors caused by STAT3 constitutive activation. The active cells with the constitutive STAT3 are utilized, so the reporter gene in the model cell is situated at an overactive state, and no additional stimulant is necessary; besides, the obtained stable cell model can be directly used for screening the compounds, thereby not only greatly reducing the screening cost, but also simplifying the whole screening process from cell cultivation, STAT3 activation, compound feed and detection to cell cultivation, compound feed and detection, shortening the screening period, reducing the operation steps, and improving the stability, efficiency and usability of the system, so that the model is more suitable for high throughput medicine screening.

In charge rate estimating apparatus and method for a secondary cell, a current flowing through the secondary cell is measured, a voltage across terminals of the secondary cell is measured, an adaptive digital filtering is carried out using a cell model in a continuous time series shown in an equation (1), all of parameters at one time are estimated, the parameters corresponding to an open-circuit voltage which is an offset term of the equation (1) and coefficients of A(s), B(s), and C(s which are transient terms, and, the charge rate is estimated from a relationship between a previously derived open-circuit voltage V0 and the charge rate SOC using the open-circuit voltage V0, V = B(s) DIVIDED A(s)-I+1 DIVIDED C(s)-V0 wherein s denotes a Laplace transform operator, A(s), B(s), and C(s) denote poly-nominal functions of s.