The invention discloses a process for producing concentrated and purified heparin, which comprises the following steps of: separating, concentrating and purifying heparin, impure protein, nucleic acid and rest sulfated polysaccharide at one time in solution of a coarse heparin product by using expansion column bed technology. The process changes the traditional process finished by multiple steps of a salt hydrolysis-ion exchange resin method. Fine heparin is separated, concentrated and purified by adopting the expansion column bed and ion exchange resin process, sedimentation is performed by adopting ethanol, dehydration is performed by adopting acetone, and the potency of the heparin is more than 180IU / mg. The expansion column bed and ion exchange resin process reduces the working procedure, shortens the time, lowers the cost, and improves the efficiency and quality.