110 results about "Polysaccharide degradation" patented technology

The invention relates to a freshness-keeping and water-retaining agent for prawns, belonging to the technical field of processing of aquatic products processing. The freshness-keeping and water-retaining agent is prepared from the following ingredients in percentage by weight: 5-10% of water-soluble chitosan, 10-20% of carboxymethyl chitosan, 10-20% of mycose, 5-10% of an antioxidant, 10-20% of konjac polysaccharide degradation product, and the balance of dextrin. For the freshness-keeping and water-retaining agent, konjac polysaccharide with strong hygroscopic property is degraded into oligosaccharide, which serves as a main auxiliary material of the water-retaining agent, the water-soluble chitosan is taken as a main auxiliary material for a bacteriostatic agent, and the carboxymethyl chitosan is taken as a film-forming agent, so as to prepare the freshness-keeping and water-retaining agent for prawns. After the freshness-keeping and water-retaining agent for prawns is applied to prawn processing, the freshness-keeping time of the prawns can be prolonged, blackening of the prawns is prevented, during refrigeration storage process of the prawns, water can be kept, and loss caused by drying loss is prevented. A processing technology of the freshness-keeping and water-retaining agent is simple, and easy to operate, and expected use effect can be achieved.

The invention discloses a method for preparing organic seaweed liquid fertilizer by fermentation and enzymolysis of microorganisms and belongs to the field of fertilizer production. Seaweeds are degraded through a compound enzymolysis technology and a microorganism fermentation technology; a complicated enzyme system produced through microorganism fermentation and a compound enzyme including proteinase, cellulase, pectinase, polysaccharide degradation enzyme and the like are used for degrading the seaweeds step by step; seaweed cell walls are removed so that contents including alginic acid, polysaccharide, plant hormones and the like are sufficiently released; the decomposition of active substances including the alginic acid, plant growth promotion factors and the like is avoided under moderate technological condition; effective active components in the seaweeds are kept to the greatest extent. According to the method disclosed by the invention, energy saving, water saving, low carbonand environment protection are realized; the seaweed biological organic liquid fertilizer contains rich mineral substances and effective components including macro-elements, microelements and the likeneeded by the growth of plants; a soil structure can be improved to keep moisture and soil and the growth of root microorganisms of the plants is promoted; the yield of applied crops is remarkably improved and the fertilizer has a great market potential.

Described herein are polysaccharide gel formulations including at least one inhibitor of polysaccharide degradation and methods of making the same. The methods described herein involve the steps of providing at least one polysaccharide and incorporating at least one inhibitor of degradation into the polysaccharide. In some embodiments, the incorporating step comprises 1 ) mixing the at least one inhibitor with the at least one polysaccharide at a highly hydrated state thereby encapsulating the at least one inhibitor in a polysaccharide network, and 2) dehydrating the polysaccharide network thereby controlling release kinetics or final swell ratio. In another embodiment, the incorporating step comprises 1) encapsulating at least one inhibitor into a biocompatible or biodegradable vessel and 2) combining the polysaccharide and the vessel into a gel formulation. The polysaccharide gel formulations described herein can be used for a variety of cosmetic applications.

Sphingomonas strains have extracellular polysaccharide (e.g., gellan, diutan) that is firmly attached to the cell surface. This attachment may limit polysaccharide production by impairing uptake of nutrients into the cell or due to limited sites for polysaccharide biosynthesis on the cell surface. Two genes for polysaccharide biosynthesis, designated gelM and gelN in gellan-producing strains and dpsM and dpsN in diutan-producing strains, have been inactivated by deletion mutations and shown to produce polysaccharide that is not firmly attached to the cell surface, i.e., slime form. Another gene for polysaccharide biosynthesis, designated gelI in gellan producing strains, was inactivated by insertion mutation and also shown to produce the slime phenotype. The homologous gene dpsi in the diutan producing strain should also be involved in the attachment of the polysaccharide to the cell surface. The slime characteristic was demonstrated by the ability of the cells to be centrifuged and the lack of cell clumping as seen under the microscope or in diluted suspensions. The diutan slime mutants had somewhat increased productivity and the recovered diutan product had significantly improved rheology. Gellan slime mutants had lower broth viscosity which facilitates mixing during fermentation; however, the recovered gellan product had lower gel strength than the gellan produced from a capsular strain. A deletion in a gene gelR, which encodes a protein with homology to surface proteins and outer membrane proteins and weak homology to proteins with polysaccharide degradation activity, was shown to restore higher gel strength to the slime form of gellan, and to produce gellan of higher gel strength than that of the capsular gellan producing strains.

The invention relates to a high-pressure water vapor degradation method for marine sulfated polysaccharides. The method is characterized in that the marine sulfated polysaccharides are prepared into water solution, then the water solution is treated by high-pressure water vapor at the temperature of 101-150 DEG C for 0.01-24hours, and the obtained treatment solution is dried for obtaining degraded products of the polysaccharides. The marine sulfated polysaccharides comprise seaweed fucoidan, carrageenan and agaropectin which are sourced from marine plants, as well as sea cucumber chondroitin sulfate, sea cucumber fucoidan and abalone sulfated polysaccharides, which are sourced from marine animals. The method has the advantages that no inorganic salt is introduced, and the final products can be obtained by drying after degradation without desalinization; the molecular weight of the polysaccharides can be reduced continuously and uniformly, and the degraded products with different molecular weight can be obtained by controlling the treatment conditions and the treatment time; the method is stable, and the reproducibility is good; and the requirement on equipment is low, and the operation is simple.

This invention relates to an extraction process of brown algae polysaccharides in a field of pharmaceutical chemistry. This invention particularly discloses a process of extracting brown algae polysaccharides based on a microwave chemistry method and brown algae polysaccharides obtained by said process. The process of the invention comprises: 1) putting pulverized brown algae powder into a microwave reaction chamber, adding acid solution to conduct reaction; optionally concentrating the mixer, and then washing with organic solvent to remove excess acid; conducting grading alcohol precipitation after water extract to obtain mannuronic acid rich fragment (M rich) algin, fucoidan and/or laminaran respectively; and adding an alkali solution to the brown algae residue to conduct alkaline digestion, filtering the residue off, adjusting pH of the filtrate to neutral, conducting alcohol precipitation to obtain guluronic acid rich fragment (G rich) algin precipitates. The present invention has significant advantages like fast processing rate, high yield of polysaccharides, strong controllable polysaccharide degradation, using less organic acid and efficient recovery, small water consumption, low power consumption, etc., the active polysaccharides has high yield and content, better water-soluble, and good biological activities.

The invention provides a preparation method and application of a low-molecular porphyra haitanensis polyferose compound. The method is characterized in that low-molecular porphyran is used as a raw material and degraded by redox reaction to obtain low-molecular-weight porphyran with the molecular weight being less than 10000Da; and the polyferose compound synthesized by heating the porphyran and ferric chloride together can be dissolved well and exist stably under a physiological pH condition, and also shows a good effect in the iron-deficiency anemia mouse model experiment. The preparation method has the advantages of being simple and convenient in operation, stable in preparation process, low in preparation cost, etc.; and the preparation method also shows wide applicability to raw materials, namely, any polysaccharide with a proper molecular weight range is applicable to the preparation method.

The invention provides a kelp degrading strain having kelp degrading activity. The strain is conserved in China General Microbiological Culture Collection Center, is classified and named as Tamlana alginolytica, and has the conservation number of CGMCC No. 5324. The provided strain is capable of generating an extracellular compound enzyme system having the activity of degrading various polysaccharides and also capable of realizing deep degradation of kelp. The invention provides two kelp juice preparation processes without adding an extracting medium and without heating and heat preservation assistance, namely a bacterial treatment process with kelp pieces as the raw material and an enzymatic treatment process with kelp powder as the raw material; the two processes both are capable of degrading kelp polysaccharides into reducing oligosaccharides or monosaccharides through biodegradation under mild conditions, and simultaneously achieving the effect of deodorization. According to the invention, the comprehensive utilization rate and eating quality of the raw material are improved and technical support is provided for refined and deep processing of kelp; as a result, the kelp degrading strain has enormous application prospect in the development aspect of kelp beverage.

The invention discloses a preparation method for rhizoma bletillae polysaccharide. The preparation method comprises the following steps: first, weighing rhizoma bletillae decoction pieces, performing hot-dipping extracting after water is added for soaking, refrigerating, filtering, and performing vacuum concentrating after the extracting liquid is de-coloured by using AB-8 resin; then performing alcohol precipitation, refrigerating, filtering, removing ethyl alcohol by nitrogen flow, and freezing and drying to obtain white and dry rhizoma bletillae polysaccharide, wherein the content of the rhizoma bletillae polysaccharide is about 85 percent, and the water solubility is good. According to the preparation method, the rhizoma bletillae polysaccharide is extracted by adopting a hot water dipping method; rhizoma bletillae polysaccharide degradation and high impurity content caused by overheating when the rhizoma bletillae polysaccharide is extracted according to a water decocting method are avoided; the problem that the requirements of deep research and development of modern medical treatment fields and novel biological materials cannot be met because of low rhizoma bletillae polysaccharide extracting efficiency, high impurity, poor colour and lustre in the conventional water decocting and alcohol precipitating method is solved; the problem that the rhizoma bletillae polysaccharide is in short supply at present is also solved.

A major object of the present technology is to provide a technology for obtaining an aloe extract which has an excellent processability into foods and contains sufficient amounts of β-sitosterol and aloe-derived dietary fibers as functional components. The present technology provides a method for manufacturing an aloe extract containing β-sitosterol, phospholipids, and dietary fibers which involves carrying out: a homogenization step for homogenizing an aloe mesophyll solution; an enzymatic degradation step for adding a polysaccharide-degrading enzyme to the homogenized aloe mesophyll solution and degrading polysaccharides contained in the aforementioned homogenized aloe mesophyll solution; and a membrane filtration step for membrane-filtering the enzyme-degraded aloe mesophyll solution with a microfiltration membrane or an ultrafiltration membrane and recovering the retentate fraction as an aloe extract.

The invention relates to a method for degrading high-molecular-weight polysaccharide containing alpha-1, 3-glycosidic bonds. The method includes: feeding a high-molecular-weight polysaccharide containing the alpha-1, 3-glycosidic bonds and of 60-200mg/mL in concentration into an electrolytic tank with a cathode and an anode; adjusting the solution to be acidic, feeding oxygen to the cathode according to an amount of 4.0-7.0 L oxygen/L polysaccharide solution, and feeding direct current and controlling current density of the cathode to be 10-12mA/cm2; degrading, and collecting electrolyte to obtain. The method has high selectivity to polysaccharide containing the alpha-1, 3-glycosidic bonds and is simple to operate, time-saving, efficient, mild in reaction condition, little to environment pollution and conducive to large-scale production. Importantly, compared with acid degradation and hydrogen peroxide degradation methods, the method has the advantages that an electric Fenton method is high in polysaccharide degradation rate, and obtained low-molecular-weight polysaccharide is narrower in molecular weight range.

The invention discloses preparation of ganoderma lucidum active polysaccharides by biofermentation degradation and an analytical method of the ganoderma lucidum active polysaccharides. The analyticalmethod comprises the following step that S1, fermentation culture of ganoderma lucidum liquid is conducted, and bacterial strain of ganoderma lucidum is firstly transferred to a plate PDA medium in anincubator with 25-30DEG C for 5-7d for activation, after the plate is covered with mycelium, a fungus block with the size of 3-6mm<2> is picked out and put into a 250mL shaking bottle with the filling capacity of 100mL for liquid fermentation culture for 7-10d to obtain a first grade seed; and 10% inoculation dose is injected into a liquid medium. A yeast fermentation method for biodegradation ofganoderma lucidum macromolecular polysaccharides, combined with an ethanol precipitation method for preparation of active low molecular weight polysaccharide components, yeast added degrades the highmolecular weight polysaccharides in the extracellular fluid into lower molecular weight polysaccharides. By a fact that Dectin-1 receptor is combined to activate NF-[kappa]B, the ganoderma lucidum active polysaccharide components are used to enhance immune activity.

The invention provides a peach gum polysaccharide degradation product PGP-1.The peach gum polysaccharide degradation product PGP-1, with molecular weight between 1X10<5> and 1X10<6>, contains no neutral polysaccharide and consists of arabinose, xylose, mannose, glucose and galactose in a molar ratio of 0.637:0.054:0.073:0.022:0.556.The peach gum polysaccharide degradation product PGP-1 is simple to prepare and capable of promoting bifidobacterium growth remarkably, inhibiting enterococcus faecium growth remarkably, scavenging hydroxyl free radicals and DPPH free radicals effectively and inhibiting Hela cell growth remarkably; Galectin-3-mediated cell agglutination inhibiting experiments show that the peach gum polysaccharide degradation product PGP-1 is capable of remarkably inhibiting agglutination of chicken blood red blood cells, Hela cells and MCF-7 cells mediated by Galectin-3.

The invention provides a method for recovering high-content acidic polysaccharide from waste liquid flowing out during column loading treatment in production of Mogroside. The method comprises the following steps: (1) waste liquid concentration; (2) alcohol precipitation; (3) separation; (4) washing; (5) redissolution; (6) column separation; (7) filtration treatment; and (8) drying. According to the invention, technological conditions are mild, and polysaccharide degradation caused by violent reaction conditions is avoided as much as possible, so the original structure of the acidic polysaccharide is reserved while high content is realized, which enables the biological activity of the acidic polysaccharide to be maintained; the whole method uses safe solvents which are easy to recycle andremove; the method is suitable for industrial production, effectively extracts the acidic Momordica grosvenori polysaccharide from the waste liquid, is high in the yield and content of the acidic polysaccharide and stable in product quality, and provides convenience for the enrichment of Momordica grosvenori products and research on the activity and structure-activity relationship of the polysaccharide.

The invention relates to a degradation method of konjac glucomannan, which comprises the following steps: preparing a water-containing konjac flour mixture of which the water content is 5-60%; and carrying out heterogeneous damp-heat degradation on the water-containing konjaku flour mixture to obtain a low-molecular-weight konjaku polysaccharide degradation product. According to the degradation method, a heterogeneous system is adopted for degradation reaction; time-consuming and energy-consuming dehydration operation after degradation of the konjac glucomannan homogeneous phase (prepared intosol) can be avoided; meanwhile, organic solvents such as ethyl alcohol used for limiting swelling of konjac polysaccharide in other heterogeneous degradation methods can be avoided, operation steps are simple, time is saved, efficiency is high, the molecular weight distribution range of degradation products can be adjusted by controlling reaction conditions, and large-scale production is facilitated.

The invention discloses a series of ulva juice products containing ulva oligosaccharide and a preparation method thereof. According to the preparation method, ulva polysaccharide which cannot be assimilated by human bodies is degraded into specific ulva oligosaccharide by utilizing polysaccharides sulfated degrading enzymes, and algae somatic cells are dispersed, thereby increasing the cell permeability, extracting effective components of ulva efficiently, obtaining the full-nutrition ulva juice, lowering the viscosity and gelling property of ulva polysaccharide and improving the mouthfeel and nutrition of the ulva products. The molecular weight distribution of oligosaccharide in the ulva juice is less than 5000Da, and the content of oligosaccharide is greater than 25mg/g (according to the dry weight of ulva). The ulva juice is then processed again so as to obtain ulva soluble products; the products can be directly developed into health beverages, seasonings and the like, also can act as raw materials to be used for the industries of food, cosmetics, health products, pharmaceuticals and the like. According to the invention, rich ulva resources are utilized fully so as to develop the ulva products which are suitable for people to take every day, so that nutritional substances in ulva can be conveniently taken by people, and the living quality of people is improved.