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Method for recovering high-content acidic polysaccharide from waste liquid flowing out during column loading treatment in production of Mogroside

A technology for acid polysaccharide and Luo Han Guo, which is applied in the field of preparing acid polysaccharide, can solve the problems of unsuitable industrial production, low content of purification process, residues in finished products, etc., and achieves the effects of mild conditions, high yield and content, and easy removal.

Inactive Publication Date: 2020-02-11
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the preparation of Luo Han Guo polysaccharides, mainly through extraction, filtration, alcohol precipitation, drying and other processes, crude polysaccharides with low purity are obtained, and macroporous resins are also used to prepare Luo Han Guo polysaccharides, but the obtained polysaccharides are heteropolysaccharides, basically all It is a neutral polysaccharide, and the simple purification process leads to low content, and some use organic solvents, which leads to the risk of residue in the finished product, and is not suitable for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] (1) Concentration of waste liquid: 10kg of Luo Han Guo waste liquid on the column was vacuum concentrated at a vacuum degree of 0.1MPa and a temperature of 75°C until the sugar content was 50brix;

[0052] (2) Alcohol precipitation: inject 6 times the volume of 90% ethanol into the concentrated solution, stir evenly, and refrigerate in a refrigerator at 0°C for 4 hours;

[0053] (3) Separation: centrifuge the cryogenically refrigerated alcohol precipitation solution at 4000r / min for 10min, take the insoluble precipitate, and carry out alcohol recovery from the clear liquid;

[0054] (4) Washing: rinse the precipitate with acetone until the eluent is clear and colorless, then vacuum-dry it under the conditions of vacuum degree -0.1MPa and temperature 70°C;

[0055] (5) Redissolution: redissolve the dried precipitate after washing into 200mL hot water at 90°C, keep warm and keep stirring for 0.5h, then centrifuge at 5000r / min for 8min and take the supernatant;

[0056] (...

Embodiment 2

[0061] (1) Concentration of waste liquid: 10kg of Luo Han Guo waste liquid on the column was vacuum concentrated at a vacuum degree of -0.8MPa and a temperature of 80°C until the sugar content was 45brix;

[0062](2) Alcohol precipitation: inject 8 times the volume of hexyl alcohol into the concentrated solution, stir evenly, and refrigerate at 4°C for 10 hours;

[0063] (3) Separation: centrifuge the cryogenically refrigerated alcohol precipitation solution at 3000r / min for 6 minutes, take the insoluble precipitate, and recover the alcohol from the clear liquid;

[0064] (4) Washing: Soxhlet reflux is performed on the precipitate with acetone until the reflux liquid is clear and colorless, and then dried under the condition of normal pressure and temperature of 80°C;

[0065] (5) Redissolution: redissolve the dried precipitate after washing into 200mL hot water at 80°C, keep warm and keep stirring for 0.3h, then centrifuge at 5000r / min for 8min and take the supernatant;

[0...

Embodiment 3

[0071] Others are the same as in Example 1, the difference is that in step (6) column separation, the upper ion exchange resin column D301, the resin volume is 200mL, the diameter to height ratio is 1:4, the upper column flow rate is 0.8bv / h, and the final product is vacuum dried to Constant weight, namely get 21.15g Luo Han Guo polysaccharide product. After testing, the acidic polysaccharides of Monk Fruit in the product obtained in Example 3 accounted for 88.60% of the total polysaccharides, and the yield was 82.19%.

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Abstract

The invention provides a method for recovering high-content acidic polysaccharide from waste liquid flowing out during column loading treatment in production of Mogroside. The method comprises the following steps: (1) waste liquid concentration; (2) alcohol precipitation; (3) separation; (4) washing; (5) redissolution; (6) column separation; (7) filtration treatment; and (8) drying. According to the invention, technological conditions are mild, and polysaccharide degradation caused by violent reaction conditions is avoided as much as possible, so the original structure of the acidic polysaccharide is reserved while high content is realized, which enables the biological activity of the acidic polysaccharide to be maintained; the whole method uses safe solvents which are easy to recycle andremove; the method is suitable for industrial production, effectively extracts the acidic Momordica grosvenori polysaccharide from the waste liquid, is high in the yield and content of the acidic polysaccharide and stable in product quality, and provides convenience for the enrichment of Momordica grosvenori products and research on the activity and structure-activity relationship of the polysaccharide.

Description

technical field [0001] The invention relates to a method for preparing acidic polysaccharides, in particular to a method for recovering high-content acidic polysaccharides from the waste liquid flowing out from the upper column of Luo Han Guo. Background technique [0002] Luo Han Guo is the ripe fruit of the genus Luo Han Guo in the family Cucurbitaceae. It has remarkable curative effects in the treatment of pharyngitis, whooping cough, acute and chronic bronchitis, and gastrointestinal diseases. It is recorded in the Pharmacopoeia of the People's Republic of China and used as a traditional Chinese medicine. Include it in the first batch of "both food and medicine list". [0003] At present, the research on Luo Han Guo mainly focuses on the sweet glycosides in it, but there are few studies on the polysaccharides contained in Luo Han Guo, especially the targeted extraction of acidic polysaccharides. [0004] Polysaccharide is a substance that exists widely in living organis...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 何安乐李伟黄华学
Owner HUNAN HUACHENG BIOTECH
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