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Biodegradation method of enteromorpha polysaccharide

A technology of prolifera polysaccharide and prolifera, which is applied in the direction of color/spectral property measurement, fermentation, etc., can solve the problem that there is no very effective method for the degradation of prolifera polysaccharide, and can achieve no by-products, short reaction time and mild action conditions. Effect

Inactive Publication Date: 2018-01-12
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some work has been done on the extraction and purification of Enteromorpha polysaccharides at home and abroad, but there is no very effective method for the degradation of Enteromorpha polysaccharides to completely degrade it into small molecular weight oligosaccharides

Method used

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  • Biodegradation method of enteromorpha polysaccharide
  • Biodegradation method of enteromorpha polysaccharide
  • Biodegradation method of enteromorpha polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-1

[0034] Weigh 0.05g of Enteromorpha crude polysaccharide and add 5mL pH of 5.0 acetic acid-sodium acetate buffer amylase solution, add 5mL100U / mL amylase solution, reaction time 2h, reaction temperature 50 ℃, solid-liquid ratio 1:2000 (enzyme concentration 50U / mL), the enzymolysis solution after enzymolysis was boiled for 5min, and the enzyme was removed by centrifugation. Clearing effect.

[0035] Change the reaction pH in Example 1-1 from 5.0 to 5.5, 6.0, 6.5, 7.0 respectively, and the rest are the same as Example 1-1 to obtain Examples 1-2, 1-3, 1-4, and 1-5.

[0036] The degraded enteromorpha polysaccharide obtained in embodiment 1-1 to 1-5 is plotted to the scavenging effect of OH figure 1 . The optimal reaction pH of amylase was 6.0.

Embodiment 2-1

[0038] Accurately weigh 0.05g of Enteromorpha crude polysaccharide, add 5mL of acetic acid-sodium acetate buffer solution with a pH of 6.0, add 5mL of 100U / mL amylase solution, reaction time 1h, reaction temperature 50°C, ratio of solid to liquid 1:2000 (enzyme concentration 50U / mL), boil the enzymolysis solution after enzymolysis for 5min, centrifuge to remove the enzyme, adjust the pH of the supernatant to neutral with alkali, and set the volume to 20mL, and use the Fenton reaction system to measure the removal of OH by Enteromorpha polysaccharide after degradation effect.

[0039] Change the reaction time in Example 2-1 from 1 h to 2, 3, 4, and 6 h respectively, and the rest are the same as Example 1-1 to obtain Examples 2-2, 2-3, 2-4, and 2-5.

[0040] The degraded Enteromorpha polysaccharide obtained in embodiment 2-1 to 2-5 is plotted to the scavenging effect of OH figure 2 . The optimal reaction time of amylase was 2h.

Embodiment 3-1

[0042]Weigh 0.05g of Enteromorpha crude polysaccharide, add 5mL of acetic acid-sodium acetate buffer solution with pH 6.0, add 5mL of 100U / mL amylase solution, reaction time 2h, reaction temperature 30°C, solid-liquid ratio 1:2000 (enzyme concentration 50U / mL), the enzymolysis solution after enzymolysis was boiled for 5 minutes, centrifuged to remove the enzyme, the supernatant was adjusted to neutral pH with alkali, and the volume was fixed to 20mL, and the Fenton reaction system was used to measure the scavenging of Enteromorpha polysaccharides to OH after degradation effect.

[0043] Change the reaction temperature in Example 3-1 from 30°C to 50, 60, 70, and 90°C respectively, and the rest are the same as in Example 3-1 to obtain Examples 3-2, 3-3, 3-4, and 3- 5.

[0044] The degraded Enteromorpha polysaccharide obtained in embodiment 3-1 to 3-5 is plotted to the scavenging effect of OH image 3 . The optimal reaction temperature of amylase was 60℃.

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Abstract

The invention specifically provides a method for completely degrading enteromorpha polysaccharide by virtue of a bio-enzyme. The method comprises the following steps: preparing crude polysaccharide into a 5mg / mL crude polysaccharide water solution; conducting a reaction at certain pH and temperature for a certain duration; boiling enzymatic hydrolysate, which is obtained after enzymatic hydrolysis, for 5min; conducting centrifuging and removing the enzyme; regulating supernatant liquid until pH value is neutral by virtue of alkali; then, dialyzing an obtained solution by virtue of a dialysis bag which is 500 in molecular weight cutoff for 24h for removing inorganic salt; and freeze-drying dialysate, so that the enzymatically degraded enteromorpha polysaccharide is obtained, wherein the molecular weight of the enteromorpha polysaccharide is 1.9645*10<3> Da and a degrading rate on the enteromorpha polysaccharide is 100%. Obtained oligosaccharide, which is low in molecular weight, is basically identical to the enteromorpha polysaccharide in chemical structure, but antioxidant activity in vitro of the oligosaccharide is obviously improved.

Description

technical field [0001] The invention relates to the technical field of marine organisms, in particular to a method for completely degrading enteromorpha polysaccharides by using enzymes. Background technique [0002] Our country is rich in algae resources and various in variety. Research on algae and development of algae resources have become the frontier of science. Enteromorpha is an important economic seaweed, which is widely distributed in the eastern coastal area and rich in resources. Polysaccharide is one of the important nutrients of Enteromorpha. It is reported that Enteromorpha polysaccharides have various activities, such as enhancing immunity, anti-tumor, lowering blood pressure, lowering blood fat, lowering blood sugar, anti-mutation, immune regulation, anti-aging, etc. These physiological activities show that Enteromorpha has broad application prospects. But Enteromorpha polysaccharide has a large molecular weight and high viscosity, so it is difficult to pas...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12P19/12C12P19/14G01N21/31
Inventor 吕海涛杜玲段科单虎秦志华
Owner QINGDAO AGRI UNIV
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